The hippocampus and cortex of mice in each group were weighed, homogenized, and then subjected to a 15 min centrifugation (3000× g) for the tissue supernatant extraction. Acquisition of cellular supernatant was accomplished from the MCM-induced cells either with or without cornuside. For the malondialdehyde (MDA) and glutathione peroxidase (GSH-Px) level and the superoxide dismutase (SOD) activity assessment, the MDA assay kit (TBA method; Jiancheng Bioengineering Institute, Nanjing, China), the GSH-Px colorimetric assay kit (Elabscience, Wuhan, China), and the T-SOD (total SOD) colorimetric assay kit (WST-1 method) were separately utilized as per the protocols of manufacturers.
Gsh px colorimetric assay kit
Manufactured by Elabscience
Sourced in China
The GSH-Px colorimetric assay kit is a laboratory tool used to measure the activity of the glutathione peroxidase (GSH-Px) enzyme. GSH-Px is an important antioxidant enzyme that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides. The kit provides a colorimetric method for the quantitative determination of GSH-Px activity in various samples, such as tissues, cells, or biological fluids.
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Lab products found in correlation
2 protocols using gsh px colorimetric assay kit
1
Oxidative Stress Biomarker Analysis
2
Antioxidant Biomarkers in Lens Homogenates
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Glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD), and glutathione peroxidase (GPx) were measured in the supernatant of the lens homogenates. GSH and MDA levels were measured with the enzyme-linked immunoassay (ELISA) kit from Elabscience (Bethesda, MD) according to the manufacturer’s instruction. SOD activity was determined with the SOD assay kit (Cayman Chemical Co., Ann Arbor, MI), and GPx activity was estimated with the GSH-PX colorimetric assay kit from Elabscience. SOD activity was presented as units per milligrams of lens tissue, and GPx was presented as unit per minute per gram of lens tissue.
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