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Growth media

Manufactured by Merck Group
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Sourced in Germany, Belgium

Growth media is a type of laboratory equipment used to culture and grow microorganisms, such as bacteria, fungi, and algae. It provides the necessary nutrients and environmental conditions for these organisms to thrive. The core function of growth media is to support the growth and proliferation of the target microorganisms, enabling researchers and scientists to study their characteristics, behaviors, and interactions.

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Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (2 mentions) Phosphate buffered saline (pbs), by Merck Group (1 mentions) Gallic acid, by Merck Group (1 mentions) Hplc grade acetonitrile, by Thermo Fisher Scientific (1 mentions) Qiaquick purification kit, by Qiagen (1 mentions) α d glucose, by Merck Group (1 mentions) Dneasy blood and tissue kit, by Qiagen (1 mentions) Genejet plasmid miniprep kit, by Thermo Fisher Scientific (1 mentions) Restriction endonuclease enzymes, by New England Biolabs (1 mentions) Ampicillin, by GoldBio (1 mentions) Imipenem, by GoldBio (1 mentions) Ethanol, by Merck Group (1 mentions) Methanol, by Merck Group (1 mentions) Miconazole, by Merck Group (1 mentions) Milli q integral water purification system for ultrapure water, by Merck Group (1 mentions) Ciprofloxacin hydrochloride, by Merck Group (1 mentions)

Spelling variants of this product

Endothelial cell growth media (4 citations) RPMI-1640 growth media (4 citations) DMEM growth media (3 citations) Minimal microbial growth medium (M9 media) (2 citations) DMEM complete growth media (2 citations) Bronchial Epithelial Growth Media (2 citations)

4 protocols using growth media

1

Microbial Staining and Analysis

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Gallic acid, crystal violet stain (powdered), phenol, dimethyl sulfoxide (DMSO) and phosphate-buffered saline (PBS) were purchased from Sigma–Aldrich® (Steinheim, Germany) and culture plates, growth media and polystyrene 24-well microplate were purchased from local market.
Albutti A., Gul M.S., Siddiqui M.F., Maqbool F., Adnan F., Ullah I., Rahman Z., Qayyum S., Shah M.A, & Salman M. (2021). Combating Biofilm by Targeting Its Formation and Dispersal Using Gallic Acid against Single and Multispecies Bacteria Causing Dental Plaque. Pathogens, 10(11), 1486.
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2

Alkaloid Precursor Acquisition for Biosynthesis Research

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(S)-Reticuline was a gift from Peter Facchini (University of Calgary). (R,S)-Norlaudanosoline was purchased from Enamine Ltd. (Kiev, Ukraine), (R)-reticuline, salutaridine, thebaine, oripavine, codeine and morphine were from TRC Inc. (North York, Ontario, Canada). Antibiotics, growth media and α-D-glucose were purchased from Sigma-Aldrich. Restriction endonuclease enzymes were from New England Biolabs (NEB). Yeast genomic DNA used as template for PCR was purified using the DNeasy Blood and Tissue Kit (Qiagen). Polymerase chain reactions (PCRs) were performed using Phusion High-Fidelity DNA polymerase (NEB/Thermo Scientific). PCR-amplified products were gel purified using the QIAquick Purification Kit (Qiagen). Plasmid extractions were done using the GeneJET plasmid mini-prep kit (Thermo Scientific). HPLC-grade water was purchased from Fluka. HPLC-grade acetonitrile was purchased from Fischer Scientific.
Fossati E., Narcross L., Ekins A., Falgueyret J.P, & Martin V.J. (2015). Synthesis of Morphinan Alkaloids in Saccharomyces cerevisiae. PLoS ONE, 10(4), e0124459.
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3

Characterization of MRSA Strains and Antibiotics

Cited 1 time
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The bacteria used in this work were obtained from the American Type Culture Collection (MRSA USA300, MRSA 33592, and MRSA 252). MRSA MW2 and MRSA MW2 ΔtarO (referenced in ref 31 (link)) were a generous gift from S. Walker.31 (link) Clinical isolates were obtained from patient swabs at the University of Oklahoma Health Sciences Center and provided by C. McCloskey following institutional review board (IRB) approval. S. aureus strains encoding the fluorescent fusion of PBP2, PBP4, and FtsZ proteins are S. aureus COL BCBPR102, S. aureus COL DpbpD BCBPR1077, S. aureus COL spa::Pspac-Ftsz-CFP-BCBAJ020, S. aureus COL PBP2::sGFP-PBP2-BCBPM073, and S. aureus COL pPBP4-YFP-BCBPM162. Chemicals from Sigma-Aldrich (DMSO, low-molecular weight branched polyethylenimine, and growth media) were used as purchased. Antibiotics (oxacillin, ampicillin, amoxicillin, ceftizoxime, imipenem, and linezolid) were purchased from Gold Biotechnology.
Hill M.A., Lam A.K., Reed P., Harney M.C., Wilson B.A., Moen E.L., Wright S.N., Pinho M.G, & Rice C.V. (2019). BPEI-Induced Delocalization of PBP4 Potentiates β‑Lactams against MRSA. Biochemistry, 58(36), 3813-3822.
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4

Preparation of Antimicrobial Test Solutions

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Ethanol, mEthanol, DMSO, growth media (components), ciprofloxacin hydrochloride, and miconazole were obtained from Sigma-Aldrich (Belgium). Water was obtained from a Milli-Q® Integral Water Purification System for ultrapure water (Millipore).
Joshi B., Panda S.K., Jouneghani R.S., Liu M., Parajuli N., Leyssen P., Neyts J, & Luyten W. (2020). Antibacterial, Antifungal, Antiviral, and Anthelmintic Activities of Medicinal Plants of Nepal Selected Based on Ethnobotanical Evidence. Evidence-based Complementary and Alternative Medicine : eCAM, 2020, 1043471.
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