IHC staining was employed following the manufacturer’s instructions (PV-6001, ZSGB-BIO, Beijing, China) using RUNX1 (1:100, 25315-1-AP, Proteintech), CCL2 (1:300, 25542-1-AP, Proteintech), CD68 (ZM-0060, ZSGB-BIO), CD163 (ZM-0428, ZSGB-BIO), F4/80 (1:800, 29414-1-AP, Proteintech) and CD31 (1:500, 28083-1-AP, Proteintech). Two independent pathologists used software ImageJ to calculate the proportion of positive area. Data were expressed as the average of three randomly selected microscopic fields.
Zm 0428
Manufactured by ZSGB-BIO
Sourced in China
The ZM-0428 is a laboratory centrifuge designed for general-purpose applications. It features a fixed-angle rotor capable of accommodating various sample tubes and containers. The centrifuge provides consistent and reliable performance to separate components in liquid samples through controlled centrifugal force.
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Lab products found in correlation
2 protocols using zm 0428
1
Immunohistochemical Analysis of Inflammatory Markers
2
Immunohistochemical Analysis of Stabilin-1
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Formalin-fixed and paraffin-embedded samples were processed for immunohistochemical analysis as previously described [23 (link), 24 (link)]. After incubation with antibodies against human stabilin-1 (AF3825, R&D system) or control antibody, the sections were incubated with secondary antibodies in an Envision System (Dako).
For triple-color immunofluorescence staining, samples were incubated with sheep anti-human stabilin-1, rabbit anti-human CD14 (10073-R001, Sino Biological) and mouse anti-human CD68 (PG-M1, Dako), or sheep anti-human stabilin-1, rabbit anti-human CD206 (ab64693, Abcam), and mouse anti-human CD163 (ZM-0428, ZSGB-BIO). Images were captured and analyzed on a Zeiss LSM710 system with the ZEN software (Zeiss, Oberkochen, Germany). Single- or double-positive cells of interest in each of two-to-five representative fields at 200 × magnification (0.16 mm2 per field) were counted manually by two independent blinded observers. Data are expressed as mean ± SEM to indicate the number of cells per field.
For triple-color immunofluorescence staining, samples were incubated with sheep anti-human stabilin-1, rabbit anti-human CD14 (10073-R001, Sino Biological) and mouse anti-human CD68 (PG-M1, Dako), or sheep anti-human stabilin-1, rabbit anti-human CD206 (ab64693, Abcam), and mouse anti-human CD163 (ZM-0428, ZSGB-BIO). Images were captured and analyzed on a Zeiss LSM710 system with the ZEN software (Zeiss, Oberkochen, Germany). Single- or double-positive cells of interest in each of two-to-five representative fields at 200 × magnification (0.16 mm2 per field) were counted manually by two independent blinded observers. Data are expressed as mean ± SEM to indicate the number of cells per field.
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