2 7 dichlorofluorescin diacetate h2dcfda 5 5 dithiobis 2 nitrobenzoic acid dtnb

RPMI 1640, fetal bovine serum (FBS), penicillin/streptomycin sulfate, amphotericin B, phosphate-buffered saline (PBS) and L-glutamine were purchased from Media Tech (Herndon, VA). Crystal violet, L glutaraldehyde, trypan blue, methamphetamine (METH) hydrochloride (MW: 185.69), 2′,7′-dichlorofluorescin diacetate (H₂DCFDA), 5,5′-dithiobis(2-nitrobenzoic acid) (DTNB), and EDTA were supplied by Sigma-Aldrich Company (St. Louis, MO). All other routine chemicals were of analytical grade.

RPMI 1640, FBS, penicillin/streptomycin sulfate, amphotericin B, PBS, and l-glutamine, were purchased from Media Tech (Herndon, VA). Crystal violet, rhodamine 123 (Rh 123), L glutaraldehyde, trypan blue, cocaine-HCl (Ecgonine methyl ester benzoate, MW: 339.8), 2′,7′–dichlorofluorescin diacetate (H₂DCFDA), 5,5-dithiobis-2-nitrobenzoic acid (DTNB), nicotinamide adenosine dinucleotide phosphate (NADPH) and ethylene diamine tetraacetic acid (EDTA) were supplied by Sigma Chemical Company (St. Louis, MO). The Nrf-2, survivin (Birc5), and GAPDH oligonucleotide primers were obtained from Eurofins Genomics (Louisville, KY). PIK-75 hydrochloride (inhibitor of Nrf-2) was purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX). Survivin inhibitor YM 155 was obtained from Calbiochem (San Diego, CA). All other routine chemicals were of analytical grade.