All cell lines were purchased from the American Type Culture Collection. WPMY1 cells were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Hyclone, Carlsbad, CA, USA) supplemented with 5% fetal bovine serum (FBS). RWPE1 cells were cultured in Keratinocyte Serum Free Medium (Gibco, Waltham, MA, USA) supplemented with 0.05 mg/mL bovine pituitary extract and 5 ng/mL epidermal growth factor. PC3, DU145, and LNCaP cells were cultured in RPMI-1640 medium (Hyclone) supplemented with 10% FBS and 1% penicillin/streptomycin (100 U/mL). Cisplatin (cis-diamminedichloroplatinum) was purchased from Sigma-Aldrich (St. Louis, MO, USA).
Cisplatin cis diamminedichloroplatinum
Manufactured by Merck Group
Sourced in United States, Denmark
Cisplatin (cis-diamminedichloroplatinum) is a platinum-based compound used in a variety of laboratory applications. It functions as a coordination complex, forming covalent bonds with DNA and inhibiting cell division. The core chemical composition and structure of Cisplatin are well-established, though its specific uses and applications may vary depending on the research or experimentation being conducted.
Automatically generated - may contain errors
Lab products found in correlation
Keratinocyte serum free medium, by Thermo Fisher Scientific (1 mentions)
Mouse igg2a, by Thermo Fisher Scientific (1 mentions)
Micro bca protein assay kit, by Thermo Fisher Scientific (1 mentions)
Ovine cholesterol, by Avanti Polar Lipids (1 mentions)
1 2 distearoyl sn glycero 3 phosphoethanolamine n maleimide polyethylene glycol 2000, by Avanti Polar Lipids (1 mentions)
2 protocols using cisplatin cis diamminedichloroplatinum
1
Cell Culture and Cisplatin Treatment
2
Targeted Lipid Nanoparticle Formulation
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Ovine cholesterol, 1,2-distearoyl-sn-glycero-3phosphocholine (DSPC), 2-distearoyl-sn-glycero-3phosphoethanolamine-N-[methoxy(polyethylene glycol)-2000] (DSPE-PEG2000) and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[maleimide(polyethylene glycol)-2000] (ammonium salt; DSPE-PEG2000-maleimide) were purchased from Avanti Polar Lipids, Inc. (Alabaster, USA); 1,1 0 -dioctadecyl-3,3,3 0 ,3 0 -tetramethylindodicarbocyanine, 4-chlorobenzenesulfonate salt (DiD), 2-iminothiolane (Traut's reagent), the Micro BCA Protein Assay Kit and mouse IgG2a were from Thermo Fisher Scientific (Hvidovre, Denmark); phosphor, platinum, gallium and iridium elemental standards were from Fluka; and cisplatin (cis-diamminedichloroplatinum) and all other chemicals were from Sigma-Aldrich (Brøndby, Denmark). The mouse anti-rat TfR antibody (OX26) was a kind gift from Professor Torben Moos (Aalborg University, Denmark).
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