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F3777

Manufactured by Agilent Technologies
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The F3777 is a high-precision laboratory instrument designed for advanced analytical applications. It features advanced technology and precise performance capabilities, but a detailed unbiased description is not available at this time.

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Lab products found in correlation

Ix71 fluorescent microscope, by Olympus (1 mentions) H 102, by Santa Cruz Biotechnology (1 mentions) Ab5694, by Abcam (1 mentions) Z0622, by Agilent Technologies (1 mentions) A21468, by Thermo Fisher Scientific (1 mentions) Ab52903, by Abcam (1 mentions) Ab290, by Santa Cruz Biotechnology (1 mentions) A1 confocal laser microscope, by Nikon (1 mentions) Sc 6556, by Santa Cruz Biotechnology (1 mentions)

2 protocols using f3777

1

Characterizing Tumor Cell Differentiation

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Three well differentiated and three poorly differentiated tumours from LSL-Kras; p53fl/+; Pdxl-Cre; Rosa-YFP (KPCY) animals were embedded in paraffin following overnight fixation. Sections were stained with antibodies against GFP (Abcam ab6673) and either αSMA (Sigma F3777), Fsp1 (DAKO A5114), Vimentin (Cell Signaling D21H3), or Zeb1 (Santa Cruz H102) using standard techniques. Images were obtained with an Olympus IX71 fluorescent microscope and quantified using Fiji software. Staining for αSMA with Abcam ab5694 (as employed by Zheng et al.) gave similar results.
Aiello N., Brabletz T., Kang Y., Nieto M.A., Weinberg R.A, & Stanger B.Z. (2017). Upholding a role for EMT in pancreatic cancer metastasis. Nature, 547(7661), E7-E8.
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2

Immunohistochemistry of Liver Tissue

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Immunohistochemistry was performed using primary antibodies against desmin (rabbit, Lab Vision Cat.No RB-9014-P, Thermo Fisher Scientific), αSMA (mouse, FITC-conjugated, Sigma-Aldrich F3777), keratin (rabbit, DAKO Z0622), keratin 19 (rat, Troma-IIIc, Developmental Studies Hybridoma Bank, University of Iowa), osteopontin (goat, R&D AF808), F4/80 (rat, AbD serotec MCA497A64), pSMAD2 (rabbit, Cell Signaling Technology, m3108, goat), pSMAD3 (rabbit, Abcam ab52903), GFP (rabbit, Abcam ab290) and HNF4α (goat, Santa Cruz Biotechnology SC-6556), and matching secondary anti-rabbit (donkey, A21207), anti-rat (chicken, A21472), anti-FITC (rabbit, A11090) and anti-goat (chicken, A21468) with various fluorescent conjugates (all Invitrogen), Confocal microscopy was performed on a Nikon A1 confocal laser microscope (Nikon Instruments) using a 20x lens or 40x and 60x oil immersion lenses.
Mu X., Pradere J.P., Affò S., Dapito D.H., Friedman R., Lefkovitch J.H, & Schwabe R.F. (2015). Epithelial Transforming Growth Factor-β Signaling Does Not Contribute to Liver Fibrosis but Protects Mice From Cholangiocarcinoma. Gastroenterology, 150(3), 720-733.
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