A 4% agarose Gel with Agarose LE (Genaxxon) was performed and pre-stained with ethidium bromide (Roth). 5 µL of dsDNA mixed with 1 µL 6x DNA Loading Dye (Thermo Scientific) and 4 µL of GeneRuler Ultra Low Range DNA Ladder (Thermo Scientific) were loaded on the gel and kept running for 15 min at 150 V. Visualization of the DNA was performed with a Genoplex system (VWR).
Genoplex system
Manufactured by Avantor
Sourced in United States
The Genoplex system is a laboratory equipment designed for performing nucleic acid extraction and purification. It provides a standardized and automated process for isolating DNA or RNA from various biological samples. The Genoplex system is intended to streamline and optimize the sample preparation workflow for downstream molecular analysis applications.
Automatically generated - may contain errors
Lab products found in correlation
Dna loading dye, by Thermo Fisher Scientific (1 mentions)
Generuler ultra low range dna ladder, by Thermo Fisher Scientific (1 mentions)
Bis tris sds page gel, by Thermo Fisher Scientific (1 mentions)
Nupage lds loading buffer, by Thermo Fisher Scientific (1 mentions)
Ecl substrate, by Bio-Rad (1 mentions)
2 protocols using genoplex system
1
Agarose Gel Electrophoresis for DNA Analysis
2
Quantification of Recombinant Salmon and Trout SAA
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Recombinant Atlantic salmon SAA protein (10 or 40 ng), recombinant rainbow trout SAA protein (40 ng), and supernatants (20 μL) from IL-1β-stimulated or control RTS-11 cells were used and were mixed with NuPAGE LDS loading buffer (Invitrogen, Waltham, MA, USA) containing 5% β-mercaptoethanol (β-ME, Sigma-Aldrich, St. Louis, MO, USA), incubated at 70 °C for 10 min, size separated in a 4–12% Bis-tris SDS-PAGE gel (Invitrogen, Waltham, MA, USA), and transferred to PVDF membrane (Amersham™, Merck, Rahway, NJ, USA). SAA protein was detected using the newly developed anti-SAA antibody and an anti-mouse-HRP-conjugated antibody (Sigma-Aldrich, St. Louis, MO, USA). The signal was revealed using ECL substrate (Biorad, Hercules, CA, USA) with GenoPlex system (VWR, Radnor, PA, USA) and quantified using Fiji v2.9.0 (NIH, Bethesda, MD, USA).
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