Sheep anti mouse nxa931

Manufactured by GE Healthcare

Sheep anti-mouse (NXA931) is a laboratory reagent designed for use in various immunoassay techniques. It is an antibody produced in sheep that specifically binds to mouse immunoglobulins. This reagent can be used as a detection tool in applications such as Western blotting, ELISA, and immunohistochemistry, where the presence or localization of mouse-derived proteins is of interest.

Automatically generated - may contain errors

Lab products found in correlation

Ab110413, by Abcam (2 mentions) Enhanced chemi luminescence (ecl), by GE Healthcare (2 mentions) Donkey anti rabbit igg na9340v, by GE Healthcare (2 mentions) Ab104274, by Abcam (1 mentions) Ab56889, by Abcam (1 mentions) Pvdf membrane, by GE Healthcare (1 mentions) A5316, by Merck Group (1 mentions) Mabn504, by Merck Group (1 mentions) Ab15246, by Abcam (1 mentions) Polyvinylidene fluoride membrane, by GE Healthcare (1 mentions)

Close spelling variants of this product

Anti-mouse NXA931 NXA931

2 protocols using sheep anti mouse nxa931

Mitochondrial Protein Analysis by Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins from mitochondrial and whole cell lysates were separated by SDS-PAGE and blotted onto PVDF membranes (GE Healthcare). The following primary antibodies were used: mouse anti–β-actin (1:5,000, A5316; Sigma-Aldrich), rabbit anti-Mitofusin1 (1:1,000, ab104274; Abcam), mouse anti-MFN2 (1:1,000, ab56889; Abcam), mouse anti-VDAC (1:5,000, MABN504; EMD Millipore), and mouse anti-OXPHOS antibody cocktail (1:1,000, ab110413; Abcam). The following secondary antibodies were used: donkey anti–rabbit IgG (NA9340V; GE Healthcare) and sheep anti–mouse (NXA931; GE Healthcare). Detection was done by incubation with HRP-conjugated secondary antibodies and conversion to chemiluminescence with ECL (GE Healthcare).

Mourier A., Motori E., Brandt T., Lagouge M., Atanassov I., Galinier A., Rappl G., Brodesser S., Hultenby K., Dieterich C, & Larsson N.G. (2015). Mitofusin 2 is required to maintain mitochondrial coenzyme Q levels. The Journal of Cell Biology, 208(4), 429-442.

+ Open protocol

+ Expand

Mitochondrial Protein Immunoblotting Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Proteins from mitochondrial and whole-cell lysates were separated by SDS-PAGE and blotted onto polyvinylidene fluoride membranes (GE Healthcare). The following primary antibodies were used: rabbit anti-α-actin (1:5,000, ab15246; Abcam), mouse anti-COQ6 (1:100, sc-393932; Santa Cruz Biotechnology), mouse anti-COQ9 (1:1,000, sc-271892; Santa Cruz Biotechnology), mouse anti-COQ5 (1:500, 17453-1-AP; Proteintech), and mouse anti-OXPHOS antibody cocktail (1:1,000, ab110413; Abcam). The following secondary antibodies were used: donkey anti-rabbit IgG (NA9340V; GE Healthcare) and sheep anti-mouse (NXA931; GE Healthcare). Detection was done by incubation with HRP-conjugated secondary antibodies and conversion to chemiluminescence with ECL (GE Healthcare).

Pujol C., Legrand A., Parodi L., Thomas P., Mochel F., Saracino D., Coarelli G., Croon M., Popovic M., Valet M., Villain N., Elshafie S., Issa M., Zuily S., Renaud M., Marelli-Tosi C., Legendre M., Trimouille A., Kemlin I., Mathieu S., Gleeson J.G., Lamari F., Galatolo D., Alkouri R., Tse C., Rodriguez D., Ewenczyk C., Fellmann F., Kuntzer T., Blond E., El Hachimi K.H., Darios F., Seyer A., Gazi A.D., Giavalisco P., Perin S., Boucher J.L., Le Corre L., Santorelli F.M., Goizet C., Zaki M.S., Picaud S., Mourier A., Steculorum S.M., Mignot C., Durr A., Trifunovic A, & Stevanin G. (2021). Implication of folate deficiency in CYP2U1 loss of function. The Journal of Experimental Medicine, 218(11), e20210846.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!