Pralidoxime

Manufactured by Merck Group

Sourced in United States

Pralidoxime is a laboratory chemical compound used as an antidote for organophosphate poisoning. It functions as a cholinesterase reactivator, helping to restore the activity of the acetylcholinesterase enzyme that is inhibited by organophosphate exposure.

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Lab products found in correlation

Heat inactivated fetal bovine serum, by Thermo Fisher Scientific (1 mentions) 2 aminoterephthalic acid, by Thermo Fisher Scientific (1 mentions) N n dimethylformamide dmf, by Chem-Lab (1 mentions) Methanol, by Avantor (1 mentions) Titanium isopropoxide, by Merck Group (1 mentions) 400 mhz nmr spectrometer, by Agilent Technologies (1 mentions)

Close spelling variants of this product

2-pralidoxime (2-PAM),

2 protocols using pralidoxime

Synthesis of 2-Aminoterephthalic Acid Titanium Oxide

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2-Aminoterephthalic acid (99%, Acros Organics, Thermo Fisher Scientific, Waltham, MA, USA), titanium isopropoxide (97%, Sigma Aldrich Co., St. Louis, MO, USA), phosphate buffered saline (PBS) solution (pH = 7.4, 0.01 M, Sigma Aldrich Co., St. Louis, MO, USA), heat-inactivated fetal bovine serum (FBS, Thermo Fisher Scientific, Waltham, MA, USA), pralidoxime (Sigma Aldrich Co., St. Louis, MO, USA), N,N’-Dimethylformamide (DMF, 99.5%, Chem-lab, Zedelgem, Belgium), methanol (analytical grade, J. T. Baker, Whitehouse Station, NJ, USA). All reagents and solvents were used as received from the commercial suppliers without further purification.

Vilela S.M., Salcedo-Abraira P., Colinet I., Salles F., de Koning M.C., Joosen M.J., Serre C, & Horcajada P. (2017). Nanometric MIL-125-NH2 Metal–Organic Framework as a Potential Nerve Agent Antidote Carrier. Nanomaterials, 7(10), 321.

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Synthesis and Characterization of Organophosphates

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Chemicals were purchased from Sigma-Aldrich and used without further purification. Electric eel AChE (EEAChE, Type V-S; EC 3.1.1.7), 5,5′-dithiobis-(2-nitrobenzoic acid) (DTNB), acetylthiocholine iodide (ATChI), diisopropyl fluorophosphate (DFP) and pralidoxime (1) were purchased from Sigma. Ethyl paraoxon and methyl paraoxon were synthesized by reaction of p-nitrophenol with diethyl or dimethyl chlorophosphate (Ghanem et al., 2007 (link)), purified to > 99% by chromatography, and characterized by NMR and high resolution mass spectrometry. ¹H NMR spectra were recorded on a Varian 400 MHz NMR spectrometer with TMS as the internal standard. Mass spectra were obtained on a Waters LCT Premier time-of-flight mass spectrometer and IR spectra on Nexus 670 FTIR spectrometer. Melting points are uncorrected.
Organophosphates are toxic reagents and should be handled in a well-ventilated hood. Methyl paraoxon, ethyl paraoxon and diisopropyl fluorophosphate may be destroyed and rendered safe by stirring with 1 N NaOH overnight at rt.

Bharate S.B., Chao C.K, & Thompson C.M. (2019). Comparison of the reactivation rates of acetylcholinesterase modified by structurally different organophosphates using novel pyridinium oximes. Environmental toxicology and pharmacology, 71, 103218.

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