α orc2

Manufactured by Bio-Rad

The α-Orc2 is a laboratory equipment product designed to facilitate research applications. It serves as a core component in various experimental setups, providing a fundamental function within the research workflow. The detailed specifications and intended use of this product are not available for an unbiased and factual presentation.

Automatically generated - may contain errors

Lab products found in correlation

α clb5, by Santa Cruz Biotechnology (3 mentions) α clb2, by Santa Cruz Biotechnology (3 mentions) α sic1, by Santa Cruz Biotechnology (3 mentions) α tubulin, by Abcam (2 mentions) α orc6, by Bio-Rad (2 mentions) α myc, by Bio-Rad (2 mentions) Clone sv5 pk1, by Abcam (2 mentions) α orc6, by Santa Cruz Biotechnology (1 mentions) Sv5 pk1, by Abcam (1 mentions)

3 protocols using α orc2

Western Blotting Protein Extraction Protocol

Cited 1 time

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Protein extracts for Western blotting were prepared following cell fixation with trichloroacetic acid and bead beating. Extracts were then separated by SDS–polyacrylamide gel electrophoresis (PAGE) and transferred to nitrocellulose membranes. Antibodies used for Western detection were α-Clb5 (Santa Cruz Biotechnology, sc20170), α-Clb2 (Santa Cruz Biotechnology, sc9071), α-Sic1 (Santa Cruz Biotechnology, sc50441), α-Orc6 (clone SB49), α-Orc2 [a gift from S. P. Bell (71 (link))], α-Rga2 and α-Boi1 [a gift from D. McCusker (51 (link))], α-Cdc24 [a gift from M. Peter (72 (link))], α-myc (clone 9E10), α–hemagglutinin (HA) (clone 12CA5), α-Pk (Bio-Rad, clone SV5-Pk1; Abcam, ab15828), and α-tubulin (Crick cell services, clone TAT-1).

Pirincci Ercan D., Chrétien F., Chakravarty P., Flynn H.R., Snijders A.P, & Uhlmann F. (2021). Budding yeast relies on G1 cyclin specificity to couple cell cycle progression with morphogenetic development. Science Advances, 7(23), eabg0007.

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Protein Extraction and Western Blot Analysis

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Protein extracts for western blotting were prepared following fixation using trichloroacetic acid57 (link). Extracts were separated by SDS-PAGE and processed by western transfer. The following antibodies were used for detection: α-Cdc14 (Ref. 45 (link)), α-Clb2 (Santa Cruz, sc-9071), α-Clb5 (Santa Cruz, sc20170), α-Sic1 (Santa Cruz, sc-50441), α-Orc6 (clone SB49), α-Orc2 (a gift from John Diffley)58 (link), α-myc (clone 9E10), α-HA (clone 12CA5), α-Pk (Bio-Rad, clone SV5-Pk1), α-tubulin (Abcam, clone YOL 1/34).

Kataria M., Mouilleron S., Seo M.H., Corbi-Verge C., Kim P.M, & Uhlmann F. (2018). A PxL motif promotes timely cell cycle substrate dephosphorylation by the Cdc14 phosphatase. Nature structural & molecular biology, 25(12), 1093-1102.

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Protein Extraction and Western Blot Analysis

Cited 1 time

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