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Multi spot mmp 3 plex ultra sensitive kit

Manufactured by Mesoscale
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The Multi‐Spot MMP 3‐Plex Ultra‐Sensitive kit is a laboratory product designed to detect and measure multiple matrix metalloproteinases (MMPs) in a sample. The kit utilizes a multiplex assay format to enable the simultaneous quantification of up to three different MMP analytes with high sensitivity.

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Lab products found in correlation

Sector imager 2400, by Mesoscale (2 mentions) Msd discovery workbench software v 4.0, by Mesoscale (2 mentions)

2 protocols using multi spot mmp 3 plex ultra sensitive kit

1

Quantifying MMP Levels in Biological Fluids

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The levels (pg/mL) of MMP‐1, MMP‐3, and MMP‐9 in diluted plasma and CSF samples (1:10) were measured with a Multi‐Spot MMP 3‐Plex Ultra‐Sensitive kit (MesoScale Discovery, USA), following manufacturer's instructions. Electrochemiluminiscent signals were read in a SECTOR Imager 2400 (MesoScale Discovery, USA) and the concentration of each metalloprotease was determined from a standard curve generated by the MSD Discovery Workbench software v. 4.0. (MesoScale Discovery, USA). Samples that fell below the detection threshold were not considered for analysis. Each sample was analyzed in three independent experiments from separate aliquots by independent investigators. The average values of all assays were used for analysis.
Pentz R., Iulita M.F., Ducatenzeiler A., Videla L., Benejam B., Carmona‐Iragui M., Blesa R., Lleó A., Fortea J, & Cuello A.C. (2020). Nerve growth factor (NGF) pathway biomarkers in Down syndrome prior to and after the onset of clinical Alzheimer's disease: A paired CSF and plasma study. Alzheimer's & Dementia, 17(4), 605-617.
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2

Quantifying Metalloprotease Levels in Biofluid Samples

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The levels (pg/ml) of MMP-1, MMP-3 and MMP-9 in diluted plasma and CSF samples (1:10) were measured with a Multi-Spot MMP 3-Plex Ultra-Sensitive kit (MesoScale Discovery, USA), following manufacturer’s instructions. Electrochemiluminiscent signals were read in a SECTOR Imager 2400 (MesoScale Discovery, USA) and the concentration of each metallo-protease was determined from a standard curve generated by the MSD Discovery Workbench software v. 4.0. (MesoScale Discovery, USA). Each sample was analyzed in three independent experiments from separate aliquots by independent investigators. The average values of all assays were used for analysis.
Pentz R., Iulita M.F., Ducatenzeiler A., Videla L., Benejam B., Carmona‐Iragui M., Blesa R., Lleó A., Fortea J, & Cuello A.C. (2020). Nerve growth factor (NGF) pathway biomarkers in Down syndrome prior to and after the onset of clinical Alzheimer's disease: A paired CSF and plasma study. Alzheimer's & Dementia, 17(4), 605-617.
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