C57bl 6 ly45.2 mice

C57Bl/6 Ly45.1 and C57Bl/6 Ly45.2 mice were purchased from Charles River Laboratory, and Cd40lg^−/− (B6.129S2‐Cd40lgtm1Imx/J) and NOD‐SCID‐IL2Rg/ (NSG) mice were purchased from The Jackson Laboratory. C57Bl/6 Ly45.1 or C57Bl/6 Ly45.1/Ly45.2 obtained by crossing C57Bl/6 Ly45.2 and C57Bl/6 Ly45.1 mice at the San Raffaele Scientific Institute animal research facility, were used as donors for adoptive T‐cell transfer and HSPC transplant into Cd40lg^−/− mice. All the mice were maintained in specific pathogen‐free (SPF) conditions, and all animal procedures were designed and performed with the approval of the Animal Care and Use Committee of the San Raffaele Hospital (IACUC #749, #818) and communicated to the Ministry of Health and local authorities according to Italian law.

C57Bl/6 Ly45.1, C57Bl/6 Ly45.2 mice were purchased from Charles River Laboratory. Cd40lg^-/- (B6.129S2-Cd40lgtm1Imx/J), humanized NSG or NSGW41 mice were purchased from The Jackson Laboratory and maintained in specific-pathogen-free (SPF) conditions. The procedures involving animals were designed and performed with the approval of the Animal Care and Use Committee of the San Raffaele Hospital (IACUC #818 for Cd40lg^-/-, #876 for NSGW41 and NSG) and communicated to the Italian Ministry of Health and local authorities according to Italian law.
Eight- to twelve- weeks old female mice were used for the experiments.