Dids 4 4 2 diisothiocyanatostilbene 2 2 2 disulfonic acid disodium salt

Manufactured by Merck Group

Sourced in France, United States

DIDS [4,4 2-Diisothiocyanatostilbene-2,2 2-disulfonic acid disodium salt] is a chemical compound used in laboratory research. It is a disulfonic acid derivative with isothiocyanate functional groups. DIDS is commonly used as an inhibitor for certain ion transport mechanisms.

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Lab products found in correlation

Carbonic anhydrase from bovine erythrocytes, by Merck Group (1 mentions) Phycoerythrin pe, by Beckman Coulter (1 mentions) Bcecf am, by Merck Group (1 mentions) Pyranine, by Merck Group (1 mentions) Protease inhibitor cocktail, by Cytiva (1 mentions) Glutathione sepharose 4b bead, by Cytiva (1 mentions) Nupage novex bis tris gel, by Thermo Fisher Scientific (1 mentions)

2 protocols using dids 4 4 2 diisothiocyanatostilbene 2 2 2 disulfonic acid disodium salt

Antibody sources and fluorescent probes

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Rabbit polyclonal antibody anti-AE1 was kindly provided by Dr C. Wagner (University of Zürich, Switzerland). Mouse monoclonal antibody anti-AE1 (BRIC6) came from the International Blood Group Reference Laboratory (IBGRL, Bristol, United Kingdom). Mouse monoclonal antibodies anti-ankyrin R (clone N388A/10) and anti-AQP1 (clone 1/A5F6) were from Neuromab (UC Davis/NIH, USA) and Bio-Rad (Marnes-la-Coquette, France) respectively, while goat polyclonal anti-stomatin (M-14) and rabbit polyclonal anti-CAII antibodies were purchased from Santa Cruz Biotechnology (Dallas, Texas USA). Secondary antibodies used in flow cytometry analysis were phycoerythrin (PE) or fluorescein isothiocyanate (FITC)-conjugated F(ab’)₂ fragment of goat anti-mouse and donkey anti-goat immunoglobulins from Beckman Coulter (Villepinte, France). BCECF-AM [2′,7′bis-(2-carboxyethyl)-5(6)-carboxyfluorescein acetoxymethyl ester] and pyranine [8-hydroxypyrene-1,3,6-trisulfonic acid] fluorescent probes, as well as carbonic anhydrase from bovine erythrocytes and the AE1 inhibitor DIDS [4,4 2-Diisothiocyanatostilbene-2,2 2-disulfonic acid disodium salt] were purchased from Sigma-Aldrich (Saint Quentin, France). Chloride indicator SPQ (6-methoxy-N-(3-sulfopropy1) Quinolinium) was obtained from Invitrogen (Fisher Scientific, Illkirch, France).

Genetet S., Desrames A., Chouali Y., Ripoche P., Lopez C, & Mouro-Chanteloup I. (2017). Stomatin modulates the activity of the Anion Exchanger 1 (AE1, SLC4A1). Scientific Reports, 7, 46170.

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Recombinant Protein Expression Optimization

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Primers used in polymerase chain reaction (PCR) and mutagenesis experiments were provided from Eurogentec (Seraing, Belgium). The QuikChange Site-Directed Mutagenesis Kit was from Stratagene (La Jolla, CA, USA). The Protease Inhibitor Cocktail, the pGEX-3X-5 vector and the glutathione-sepharose 4B beads were purchased from Amersham Pharmacia Biotech, (Buckinghamshire, UK). NuPAGE® Novex Bis-Tris Gels were purchased from Invitrogen (Carlsbad, CA, USA). The Band 3 inhibitor, DIDS (4,4 2-diisothiocyanatostilbene-2,2 2-disulfonic acid disodium salt), was purchased from Sigma-Aldrich (St Louis, MO, USA).

Azouzi S., Collec E., Mohandas N., An X., Colin Y, & Le Van Kim C. (2015). The human Kell blood group binds the erythroid 4.1R protein: new insights into the 4.1R-dependent red cell membrane complex. British journal of haematology, 171(5), 862-871.

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