Log In Sign up
The largest database of trusted experimental protocols

Rnf20

Manufactured by Cell Signaling Technology
Visit Supplier
Sourced in Sweden

RNF20 is a protein that functions as an E3 ubiquitin-protein ligase. It plays a role in regulating gene expression and DNA repair processes.

Automatically generated - may contain errors

Lab products found in correlation

510 confocal microscope, by Zeiss (2 mentions) Ubiquitin lysine 48, by Merck Group (2 mentions) Zscan4, by OriGene (2 mentions) Brca1, by Merck Group (1 mentions) P chk1, by Cell Signaling Technology (1 mentions) P atr, by Cell Signaling Technology (1 mentions) Rad51, by Cell Signaling Technology (1 mentions) Fibrillarin, by Cell Signaling Technology (1 mentions) Ruvbl2, by Fortis Life Sciences (1 mentions) H2bk120ub, by Cell Signaling Technology (1 mentions) P atm, by Cell Signaling Technology (1 mentions) γh2ax, by Merck Group (1 mentions) Gapdh, by Cell Signaling Technology (1 mentions)

3 protocols using rnf20

1

Immunohistochemical Analysis of Epigenetic Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in ice cold methanol/acetic acid (3:1). The fixed cells were dropped on microscope slides. Antigen retrieval was performed followed by blocking for 10 min at room temperature. Primary antibody was incubated overnight at 4 °C for the following antigens: ZSCAN4 (Origene, 1:1000), RNF20 (Cell Signaling, 1:2000), and Ubiquitin Lysine 48 (Millipore 1: 2000). The slides were washed and incubated with secondary antibodies conjugated with Alexa-488 or 568 at room temperature for 1 h and then treated with DAPI and To-Pro-3 to stain the nuclei. Slides were mounted and visualized by a Zeiss 510-confocal microscope. Co-localization analyses were performed by ImageJ software [16 (link)].
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
+ Open protocol
+ Expand
2

Multimodal Analysis of DNA Damage Signaling

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used: ATR (sc1887 N19); RAD51 (sc8349); RPA (Cell Signaling Technology, Danvers, MA, USA, 4E4); P-ATM (sc47739); H2B (ab52484); H2BK120Ub (Cell Signaling Technology, D11); H3 (sc10809); Actin (ab6276); CDC73 (Cell Signaling Technology, D38E12); P-RPA (Bethyl Laboratories NB100-S44); P-ATR (Cell Signaling Technology, S428); P-Chk1 (Cell Signaling Technology, 133D3); Chk1 (Cell Signaling Technology, 261DS); 53BP1 (ab36823); GFP (sc8334); PARP1 (sc7007 F2); KU70 (sc17789 E5); CtIP (Bethyl Laboratories, Inc., Montgomery, TX, USA,A300-488A); BRCA1 (sc642); γH2AX (Millipore, Merck Chemicals and Life Science AB, Solna, Sweden, JBW301); PAF1 (ab137519); GAPDH (sc365062); KU86 (sc528); Fibrillarin (ab5821); UBA1 (Cell Signaling, 4890); CAND1 (Bethyl Laboratories, Inc. A302-901A-T); RNF20 (Cell Signaling Technology, 9425); RNF40 (Bethyl Laboratories, A300-719A-T); RUVBL2 (Bethyl Laboratories, A302-536A-T); CUL1 (Bethyl Laboratories, A303-373A-T); FBXO21 (ab179818); Flour-555 (A21434, Invitrogen, Life Technologies Europe BV, Stockholm, Sweden); Alexa Flour-488; Phalloidin 594 (Sigma-Aldrich Company Ltd, Dorset, UK, 51927); mouse, rabbit, rat horseradish peroxidase-conjugated ab (Abcam, Cambridge, UK); mouse and rabbit IRDye-conjugated ab (Licor, Lincoln, NE, USA).
Herr P., Lundin C., Evers B., Ebner D., Bauerschmidt C., Kingham G., Palmai-Pallag T., Mortusewicz O., Frings O., Sonnhammer E, & Helleday T. (2015). A genome-wide IR-induced RAD51 foci RNAi screen identifies CDC73 involved in chromatin remodeling for DNA repair. Cell Discovery, 1, 15034-.
+ Open protocol
+ Expand
3

Immunohistochemical Analysis of Epigenetic Regulators

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were fixed in ice cold methanol/acetic acid (3:1). The fixed cells were dropped on microscope slides. Antigen retrieval was performed followed by blocking for 10 min at room temperature. Primary antibody was incubated overnight at 4 °C for the following antigens: ZSCAN4 (Origene, 1:1000), RNF20 (Cell Signaling, 1:2000), and Ubiquitin Lysine 48 (Millipore 1: 2000). The slides were washed and incubated with secondary antibodies conjugated with Alexa-488 or 568 at room temperature for 1 h and then treated with DAPI and To-Pro-3 to stain the nuclei. Slides were mounted and visualized by a Zeiss 510-confocal microscope. Co-localization analyses were performed by ImageJ software [16 (link)].
Portney B.A., Khatri R., Meltzer W.A., Mariano J.M, & Zalzman M. (2018). ZSCAN4 is negatively regulated by the ubiquitin-proteasome system and the E3 ubiquitin ligase RNF20. Biochemical and biophysical research communications, 498(1), 72-78.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!