Antigen retrieval buffer

Manufactured by R&D Systems

Antigen retrieval buffer is a laboratory reagent used to prepare tissue samples for immunohistochemical analysis. It helps expose target antigens in fixed tissue sections, enhancing the ability to detect and visualize these molecules using specific antibodies. The buffer's core function is to facilitate antigen retrieval, a crucial step in the immunohistochemical staining process.

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Lab products found in correlation

Peptide n glycosidase f pngase f, by New England Biolabs (1 mentions) N n dimethylaniline, by Merck Group (1 mentions) 2 5 dihydroxybenzoic acid, by Merck Group (1 mentions) Dithiothreitol (dtt), by New England Biolabs (1 mentions) Peroxidase blocking reagent, by Agilent Technologies (1 mentions) Maltoheptaose, by Merck Group (1 mentions) Abc elite kit, by Vector Laboratories (1 mentions) Intercalator ir, by Standard BioTools (1 mentions)

Spelling variants of this product

Antigen Retrieval Reagent-Acidic buffer (pH 6.0) (2 citations)

2 protocols using antigen retrieval buffer

Glycosylation Analysis of Proteins

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Antigen retrieval buffer was
purchased from R&D Systems. Peptide-N-Glycosidase F (PNGase F)
was from New England Biolabs, dithiothreitol (DTT), maltoheptaose
(DP7), 2,5-dihydroxybenzoic acid (DHB), and N,N-Dimethylaniline
(DMA) were purchased from Sigma-Aldrich. Biotinylated AAL and ConA
lectins and the ABC-Elite kit were from Vector Laboratories. Peroxidase
blocking reagent was from Dako.

Toghi Eshghi S., Yang S., Wang X., Shah P., Li X, & Zhang H. (2014). Imaging of N-Linked Glycans from Formalin-Fixed Paraffin-Embedded Tissue Sections Using MALDI Mass Spectrometry. ACS Chemical Biology, 9(9), 2149-2156.

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Multiplexed Immunohistochemistry of FFPE Samples

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FFPE sections were dewaxed and rehydrated as routine. Heat-induced epitope retrieval was conducted in a water bath at 95 °C in antigen retrieval buffer (R&D Systems Inc., Minneapolis, MN) for 10 min. After immediate cooling, the sections were blocked with 3% BSA in PBS for 45 min. Samples were incubated overnight at 4 °C with a final concentration of 5–10 μg/mL of metal-conjugated antibody cocktails diluted in PBS/0.5% BSA (Table 1). Samples were then washed with 2× PBS/0.1% Triton X-100 and 2× PBS and exposed to 25 μM Ir-Intercalator (Fluidigm) for 30 min at RT for nuclei staining. The samples were rinsed twice in distilled water and air-dried before IMC analysis.

Chang Q., Ornatsky O.I., Siddiqui I., Straus R., Baranov V.I, & Hedley D.W. (2016). Biodistribution of cisplatin revealed by imaging mass cytometry identifies extensive collagen binding in tumor and normal tissues. Scientific Reports, 6, 36641.

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