Anti mouse caspase 1 p20 ag 20b 0042

Manufactured by Adipogen

Anti-mouse caspase-1 p20 (AG-20B-0042) is a laboratory reagent used for research purposes. It is a recombinant protein that corresponds to the p20 subunit of mouse caspase-1.

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Lab products found in correlation

Nigericin, by Merck Group (4 mentions) Anti nlrp3 ag 20b 0014, by Adipogen (4 mentions) Ultrapure lps, by Thermo Fisher Scientific (3 mentions) Anti mouse il 1β p17 af 401 na, by R&D Systems (3 mentions) Anti nek7 sc 50756, by Santa Cruz Biotechnology (3 mentions) Mitosox, by Thermo Fisher Scientific (3 mentions) Adenosine triphosphate (atp), by Merck Group (3 mentions) Anti mouse il 1β af 401 na, by R&D Systems (2 mentions) Poly a t, by Merck Group (2 mentions) Pam3csk4, by Thermo Fisher Scientific (2 mentions) Protein g agarose, by Merck Group (2 mentions) Streptavidin coated beads, by Merck Group (2 mentions) Anti asc sc 22514 r, by Santa Cruz Biotechnology (2 mentions) Anti β actin p30002, by Abmart (2 mentions) Anti human cleaved il 1β a5208206, by Sangon (2 mentions) Anti flag f2555, by Merck Group (2 mentions) Anti human caspase 1, by Cell Signaling Technology (2 mentions) Mitotracker, by Thermo Fisher Scientific (2 mentions) Mouse il 1β elisa kit, by BD (1 mentions) Anti mouse nlrp3 ag 20b 0014, by Adipogen (1 mentions)

Close spelling variants of this product

Anti-mouse caspase-1 (AG-20B-0042) Anti-caspase-1 (AG-20B-0042) Caspase-1(AG-20B-0042) AG-20B-0042 Mouse anti-caspase 1 (p20) Mouse caspase-1 p20 Anti-caspase-1 p20 Caspase-1 p20 Anti-mouse caspase-1 Mouse caspase-1

6 protocols using anti mouse caspase 1 p20 ag 20b 0042

Inflammasome Activation Assay Protocol

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LPS (O111:B4, L2630), ATP (A7699), disuccinimidyl suberate (S1885), nigericin (N7143), and phorbol myristate acetate (PMA, P1585) were from Sigma-Aldrich; alum (77161) and the c-Myc tag (MA1-980) were from Thermo Fisher Scientific; and poly(dA:dT) was from InvivoGen. The following antibodies were used: anti-Flag (F1804) (Sigma); anti-mouse caspase-1 p20 (AG-20B-0042) and anti-mouse NLRP3 (AG-20B-0014) (AdipoGen); anti-human caspase-1 (ab108362) and anti-BRCC36 (ab108411) (Abcam); anti-mouse IL-1β (AF-401-NA) (R&D); anti-HA-tag (3724) (Cell Signaling Technology); and anti-VDR (sc-13133), anti-ASC (sc-514414), and anti-β-actin (sc-47778) (Santa Cruz). The Mouse TNF ELISA Kit (558534), Mouse IL-1β ELISA Kit (559603), and TMB Substrate Reagent Set (555214) were from BD Biosciences. The mouse IL-18 ELISA set (EMC06) was from ExCell Biotech (Table 1).

Rao Z., Chen X., Wu J., Xiao M., Zhang J., Wang B., Fang L., Zhang H., Wang X., Yang S, & Chen Y. (2019). Vitamin D Receptor Inhibits NLRP3 Activation by Impeding Its BRCC3-Mediated Deubiquitination. Frontiers in Immunology, 10, 2783.

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Inflammasome Activation Assay Protocol

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HDM was from Greer Laboratories. Anti-mouse IL-1β (p17) (AF-401-NA) was from R&D. Anti-mouse caspase-1 (p20) (AG-20B-0042) and anti-NLRP3 (AG-20B-0014) antibodies were from Adipogen. Anti-ASC (67824) antibody was from Cell Signaling Technology. Anti-β-actin (66009-1-Ig) was from Proteintech.
Anti-mouse antibodies used for flow cytometry were: CD3-FITC (BD, 553062, 145-2C11), CD19-FITC (eBioscience, 11-0193-82, 1D3), Ly-6G-PE (Biolegend, 127608, 1A8), CD45-PE (eBioscience, 12-0451-81, 30-F11), CD11c-PerCP-Cy5.5 (Biolegend, 117328, N418), CD11b-PerCP-Cy5.5 (BD, 550993, M1/70), CD11b-PE-Cy7 (eBioscience, 25-0112-82, M1/70), CD3e-PE-Cy7 (BD, 552774, 145-2C11), CD19-PE-Cy7 (Biolegend, 115520, 6D5), SiglecF-Alexa Fluor 647 (BD, 562680, E50-2440), MHCII-APC-eFluor 780 (eBioscience, 47-5321-82, M5/114.15.2), Ly-6G-BV421 (Biolegend, 127628, 1A8), CD45-BV510 (Biolegend, 103137, 30-F11), CD11c-BV510 (Biolegend, 117338, N418). Ultrapure LPS was obtained from Invitrogen. Nigericin was obtained from Sigma-Aldrich. RRx-001 (S8405) was from Selleck.

Ma M., Li G., Qi M., Jiang W, & Zhou R. (2021). Inhibition of the Inflammasome Activity of NLRP3 Attenuates HDM-Induced Allergic Asthma. Frontiers in Immunology, 12, 718779.

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Cytokine Activation Pathway Analysis

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Phorbol-12-myristate-13-acetate (PMA), MSU, ATP, nigericin, poly A:T, Pam3CSK4, A9C, DIDS, and IAA94 were purchased from Sigma. MitoSox, MitoTracker Red, DAPI, MQAE, Lipofectamine RNAiMAX, Lipofectamine 2000, and M-MLV were from Invitrogen. Ultrapure LPS was obtained from Invivogen. SYRB Green was from TransGen Biotech. MnTBAP was from Santa Cruz. Salmonella is a gift from R.V. Bruggen. Anti-human cleaved IL-1β (2021, 1:1000 dilution) and anti-human caspase-1(2225, 1:1000 dilution) antibodies were purchased from Cell Signalling. Anti-human pro-IL-1β (60136-1-lg, 1:1000 dilution) anti-CLIC1(14545-1-AP, 1:500 dilution), anti-GAPDH (10494-1-AP, 1:1000 dilution), anti-VDAC1 (66345-1-lg, 1:1000 dilution), and anti-ATP1A1 (55187-1-AP, 1:1000 dilution) antibodies were from Proteintech. Anti-CLIC4 (SC-135739, 1:1000 dilution), anti-NEK7 (SC-50756, 1:500 dilution), and anti-ASC (rabbit, SC-22514, 1:400 dilution) antibodies were from Santa Cruz. Anti-ASC (mouse, 04–147, 1:1000 dilution) antibody was from Merck Millipore. Anti-CLIC5 (ACL-025, 1:250 dilution) antibody was from Alomone labs. Anti-mouse IL-1β (AF-401-NA, 1:1000 dilution) antibody was from R&D. Anti-mouse caspase-1 (p20) (AG-20B-0042, 1:1000 dilution) and anti-NLRP3 (AG-20B-0014, 1:1000 dilution) antibodies were from Adipogen. Anti-β-actin (P30002, 1:1000 dilution) antibody was from Abmart.

Tang T., Lang X., Xu C., Wang X., Gong T., Yang Y., Cui J., Bai L., Wang J., Jiang W, & Zhou R. (2017). CLICs-dependent chloride efflux is an essential and proximal upstream event for NLRP3 inflammasome activation. Nature Communications, 8, 202.

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NLRP3 Inflammasome Activation Pathway

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MSU, nigericin, ATP, PMA, BHB, DL-sulforaphane, poly(dA:dT), and glucose were purchased from Sigma-Aldrich. MCC950 was acquired from Selleck. BAY 11-7082, isoliquiritigenin, parthenolide, flufenamic acid, and mefenamic acid were acquired from Topscience. Human recombinant insulin was bought from Novo Nordisk. The One Touch Ultra Blood glucose Test System kit was bought from Roche. Ultrapure LPS, Pam3CSK4, MitoTracker, and MitoSOX were obtained from Invitrogen. Protein G agarose and streptavidin-coated beads were respectively supplied by Millipore and Pierce Biochemicals. ATP-coupled beads were from BioWorld Company. Anti-mouse caspase-1 (p20; AG-20B-0042) and anti-NLRP3 (AG-20B-0014) were from Adipogen. Anti-ASC (sc-22514-R) and anti-NEK7 (SC-50756) were from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17; AF-401-NA) was from R&D Systems. Anti-Flag (F2555) and anti-Mcherry (V4888) were from Sigma-Aldrich. Recombinant human NLRP3 was from Novus Biologicals. A standard HFD (D12492, 60% kcal fat) was from Research Diet Company.

Jiang H., He H., Chen Y., Huang W., Cheng J., Ye J., Wang A., Tao J., Wang C., Liu Q., Jin T., Jiang W., Deng X, & Zhou R. (2017). Identification of a selective and direct NLRP3 inhibitor to treat inflammatory disorders. The Journal of Experimental Medicine, 214(11), 3219-3238.

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Antibody Reagents for IL-1β and Clec12a

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Anti-human IL-1β functional grade purified antibody (16-7018-85), anti-IL-1beta monoclonal antibody (13-7016-85), anti-mouse IL-1beta capture antibody were obtained from eBioscience; anti-Clec12a (C-terminal) (ab193941) was obtained from Abcam; HRP-conjugated anti-mouse IgG (7076), β-actin (13 × 10⁵) rabbit mAb (4970) and anti-human Caspase-1 were obtained from CST; anti-mouse Caspase-1 p20 (AG-20B-0042) was obtained from Adipogen; human MICL/Clec12a antibody (MAB29461-100) and mouse MICL/Clec12a antibody (AF2950) were obtained from R&D systems; PE-conjugated rat anti-mouse CD371 (Clec12a) antibody (562773) was obtained from BD biosciences; PE conjugated anti-human CD371 (Clec12a) monoclonal antibody (HB3) (12-9878-42), allophycocyanin-conjugated p-Syk (Tyr348) monoclonal antibody (mono1ct) (17-9014-42), fluorescein isothiocyanate-conjugated goat anti-rabbit IgG F(ab′)2 secondary antibody (31573), and fluorescein isothiocyanate-conjugated rabbit anti-goat IgG F(ab′)2 secondary antibody (31553) were obtained from Invitrogen; HA-tag (26D11) mouse monoclonal antibody (M20003L) was obtained from Abmart; mouse IgG1 isotype control antibody (130-113-762) was obtained from MACS.

Xu Y., Song D., Wang W., Li S., Yue T., Xia T, & Shi Y. (2023). Clec12a inhibits MSU-induced immune activation through lipid raft expulsion. Life Science Alliance, 6(9), e202301938.

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NLRP3 Inflammasome Activation and IL-1β Regulation

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ATP, MSU, poly A:T, nigericin, and glucose were from Sigma. The human recombinant insulin was from Nova Nordisk. One Touch^® Ultra^® Blood glucose Test System was from Roche. Oridonin (s2335) was bought from Selleck. MitoSOX, ultrapure LPS, Pam3CSK4 and MitoTracker were from Invitrogen. Protein G agarose and streptavidin-coated beads were, respectively, supplied by Millipore and Pierce Biochemicals. Anti-Flag (F2555) and anti-VSV (V4888) were bought from Sigma. Anti-NLRP3 (AG-20B-0014) and anti-mouse caspase-1 (p20) (AG-20B-0042) antibodies were from Adipogen. Anti-NEK7 (SC-50756) and anti-ASC (sc-22514-R) antibodies were obtained from Santa Cruz. Anti-β-actin (P30002) was bought from Abmart. Anti-human caspase-1 was from Cell Signaling Technology. Anti-human cleaved IL-1β (A5208206) was from Sangon Biotech. Anti-mouse IL-1β (p17) (AF-401-NA) was from R&D Systems. A standard high-fat diet (D12492, 60% kcal fat) was from Research Diet Company.

He H., Jiang H., Chen Y., Ye J., Wang A., Wang C., Liu Q., Liang G., Deng X., Jiang W, & Zhou R. (2018). Oridonin is a covalent NLRP3 inhibitor with strong anti-inflammasome activity. Nature Communications, 9, 2550.

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