Inbred male Lewis rats weighing 250–300 g (Charles River Laboratories, Boston, MA, USA) were used as both graft donors and recipients for syngeneic orthotopic liver transplantation. The animals were housed in accordance with National Research Council guidelines, and the experimental protocols were approved by the institutional animal care and use committee at Duke University (Durham, NC, USA). The animals were divided into three groups: the DCD30 + 1 h acRTMP group (n = 8, 30 min WIT, 1 h acRTMP, and subsequent transplantation), the Control + 1 h acRTMP group (n = 8, 0 min WIT, 1 h acRTMP, and transplantation), the DCD30 + 1 h SCS group (n = 6, 30 min WIT, 1 h of SCS in HTK solution, and transplantation). Experimental protocol is indicated in Figure 1 .
Inbred male lewis rats
Manufactured by Charles River Laboratories
Sourced in United States, Germany, Italy
Inbred male Lewis rats are a laboratory animal model used in biomedical research. They are a well-characterized strain that provides consistent and reliable results for studies. The core function of these rats is to serve as a standardized experimental subject to support scientific investigations.
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13 protocols using inbred male lewis rats
1
Syngeneic Orthotopic Liver Transplantation in Rats
2
Rat Liver Subnormothermic Machine Perfusion
3
Rat Liver Subnormothermic Machine Perfusion
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Inbred male Lewis rats weighing 250 to 300 g (Charles River Laboratories, Boston, MA, USA) were used for transplantation. The animals were maintained in accordance with National Research Council guidelines, and the experimental protocols were approved by the IACUC at Massachusetts General Hospital (Boston, MA, USA). After dissection of the ligaments surrounding the liver, the intrahepatic inferior vena cava (IHIVC) and portal vein (PV) were elongated by dissecting their distributive veins (right renal and adrenal veins, lumbar venous plexus, gastroduodenal and splenic veins). The left diaphragmatic vein was ligated. The hepatic artery was ligated and cut. The common bile duct was cannulated for bile collection and dissected. The portal vein was cannulated and the liver was perfused with 10 mL, 21 °C oxygenated Williams medium E, excised from its recess and transferred to the subnormothermic machine perfusion (SNMP) system. The portal vein was cuffed using a modified 22G intravenous catheter.
4
Streptozotocin-induced Diabetic Rat Model
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Inbred male Lewis rats, weighing 275–300 g, (Charles River, Calco, Italy) were used as donors and recipient of the grafts. The animals were fed on standard rodent chow (Rieper, Bolzano, Italy) and water ad lib, and were kept under a 12 h light/dark cycle. Rats were made diabetic by streptozotocin (STZ, Sigma-Aldrich, Milano, Italy, 65 mg/Kg i.p. injection). Blood glucose concentration was determined by means of a commercially available human glucose meter (Stat Strip Xpress, Nova Biomedical UK). Animals with fasting blood glucose higher than 16.7 mmol/l (>300 mg/dL) on at least two consecutive measures were considered diabetic while rats that remained lower than 16.7 mmol/l after 1 week were withdrawn from the study. All the experimental procedures were carried out with the approval of the ethical committee for animal experimentation of the University of Pisa.
5
Inbred Lewis Rat Experiment
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Inbred male Lewis rats (n=6 per group) weighing 200–250 g were obtained from Charles River (Sulzfeld, Germany). All procedures were performed according to the Guide for the Care and Use of Laboratory Animals published by the National Academy of Sciences and were approved by the local authorities (Regional Council Karlsruhe, Germany (reference number 35-9185.81/G -64/09)).
6
Inbred Male Lewis Rat Study
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Inbred male Lewis rats, weighing 220–300 g, were purchased from Charles River Laboratories International, Inc. (Kanagawa, Japan) and SLC Japan (Hamamatsu, Japan). All rats were maintained in a specific-pathogen-free environment and housed under a 12-h dark–light cycle (light from 07:00 to 19:00) at 22 ± 1 °C. They were given free access to standard diet and water and were not fasted before the experiments.
7
Male Lewis Rat Experiments
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Forty-three inbred, male Lewis rats (250 ± 50 grams) were used for all experiments (Charles River Laboratories, Wilmington, MA). The animals received humane care in accordance with the National Research Council guidelines and the experimental protocols were approved by the IACUC of Massachusetts General Hospital (Boston, MA) and the Animal Care and Use Review Office. Authors complied with the ARRIVE guidelines.
8
Inbred Male Lewis Rat Care
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Inbred male Lewis rats (8–12 weeks old; Charles River Laboratories, Beijing, China) received care in compliance with the Guide for the Care and Use of Laboratory Animals (National Institutes of Health Publication No. 85-23, revised 1996). All procedures and handling of animals during the investigations were reviewed and approved by the Ethical Committee of Laboratory Animal Research Center of Southern Medical University Nanfang Hospital. The animals were housed at constant ambient temperature (22 ± 2 °C) in light-controlled rooms (12–12-h light-dark cycles), were given food and water access ad libitum, and acclimatized for 1 week.
9
Male Rat Anesthesia and Analgesia
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Male inbred Lewis rats (300 ± 50 g; Charles River, Sulzfeld, Germany) were used in this study. All animals were housed under standard animal care conditions and had access to water and rat chow ad libitum. Male rats were used to avoid hormonal fluctuations. The permission for animal experiments was given by the “Thüringer Landesamt für Verbraucherschutz” (AZ: 2226840402026/13).
Animals were allowed to adapt to laboratory conditions for at least seven days. All procedures were carried out according to German Animal Welfare Legislation and were performed under inhalation anesthesia with 3% isoflurane (Sigma Delta, London, UK) complemented by the injection of the analgesic drug Torbugesic Vet (5 μg/kg).
Animals were allowed to adapt to laboratory conditions for at least seven days. All procedures were carried out according to German Animal Welfare Legislation and were performed under inhalation anesthesia with 3% isoflurane (Sigma Delta, London, UK) complemented by the injection of the analgesic drug Torbugesic Vet (5 μg/kg).
10
Inbred Lewis Rat Model for Evaluation
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The study was approved by the local Ethics Committee and by the Italian Ministry of Health according to Italian Law (D.lgs 116/92, article 7), and all procedures were carried out according to European legislation following the guidelines for care and use of laboratory animals.
Male inbred Lewis rats (weight range 250–300 g) from Charles River Laboratories (Lecco, Italy) and male green fluorescent protein (GFP) transgenic Lewis rats from RRRC (Columbia, MO, USA) were used. All animals were housed in single cages with a natural night and day cycle, free access to water, and a commercial pellet diet (Harlan, Udine, Italy) ad libitum. Preoperative and postoperative clinical evaluation was carried out and the feeding and defecation behavior was observed daily to verify fecal continence and detect possible complications. Rats were euthanized using an anesthetic overdose followed by exsanguination 30 days after treatment.
Male inbred Lewis rats (weight range 250–300 g) from Charles River Laboratories (Lecco, Italy) and male green fluorescent protein (GFP) transgenic Lewis rats from RRRC (Columbia, MO, USA) were used. All animals were housed in single cages with a natural night and day cycle, free access to water, and a commercial pellet diet (Harlan, Udine, Italy) ad libitum. Preoperative and postoperative clinical evaluation was carried out and the feeding and defecation behavior was observed daily to verify fecal continence and detect possible complications. Rats were euthanized using an anesthetic overdose followed by exsanguination 30 days after treatment.
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