Lipopolysaccharide lps from salmonella enterica serotype enteritidis

Chitosan was obtained from Sigma-Aldrich (St. Louis, MO, USA), Acros Organics (Bridgewater, NJ, USA), AK Scientific (Union City, CA, USA), MP Biomedicals (Solon, Ohio, OH, USA), Spectrum Chemical Mfg Corp. (New Brunswick, NJ, USA) and Primex (Siglufjordor, Iceland). Purified, low endotoxin Chitosan (<0.01 EU/mg) was obtained from the University of Arkansas Biologics Center (UABC) (Fayetteville, AR, USA). Sodium hydroxide was purchased from Thermo Fisher Scientific Inc. (Waltham, MA, USA). Hydrochloric acid and acetic acid were purchased from Sigma-Aldrich (St. Louis, MO, USA). Unless otherwise specified, Chitosan solutions were prepared by dissolving Chitosan in 0.1 M HCl and adjusted to pH 6.0 using NaOH.
Cell culture media components including Dulbecco’s modified Eagle’s medium (DMEM), Roswell Park Memorial Institute 1640 (RPMI-1640), fetal bovine serum (FBS), l-glutamine and penicillin-streptomycin solution were purchased from Hyclone Laboratories (Logan, UT, USA). Ammonium-chloride-potassium (ACK) buffer used in the process of isolating bone marrow derived dendritic cells from mouse was purchased from Lonza (Allendale, NJ, USA). Recombinant murine granulocyte-macrophage colony stimulating factor (rmGM-CSF) was purchased from Peprotech (Rocky Hill, NJ, USA). Lipopolysaccharide (LPS) from Salmonella enterica serotype enteritidis was purchased from Sigma-Aldrich.