Hsp27 sirna

Manufactured by Thermo Fisher Scientific

HSP27 siRNA is a laboratory reagent designed for gene silencing experiments. It is a small interfering RNA (siRNA) that targets the HSP27 (heat shock protein 27) gene, which is involved in the cellular stress response. HSP27 siRNA can be used to temporarily reduce the expression of the HSP27 gene in cell culture experiments.

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Lab products found in correlation

Streptomycin, by Thermo Fisher Scientific (2 mentions) Potassium penicillin g, by Thermo Fisher Scientific (2 mentions) Fungizone, by Thermo Fisher Scientific (2 mentions) Glutamine, by Thermo Fisher Scientific (2 mentions) Tnf α, by R&D Systems (2 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (2 mentions) Sb203580, by Santa Cruz Biotechnology (1 mentions) Cid 755673, by Merck Group (1 mentions)

4 protocols using hsp27 sirna

Cell Culture and Stimulation Protocol

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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin G potassium, streptomycin, fungizone, and glutamine were purchased from Invitrogen (Carlsbad, CA). TNF-α was purchased from R&D Systems (Minneapolis, MN). HSP27-siRNA was purchased from Ambion (Carlsbad, CA). NS398 was purchased from Calbiochem (San Diego, CA). SB203580 was purchased from Santa Cruz (Dallas, TX).

Saini S., Liu T, & Yoo J. (2016). TNF-α Stimulates Colonic Myofibroblast Migration via COX-2 and Hsp27. The Journal of surgical research, 204(1), 145-152.

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Silencing of Hsp27 in CCD-18Co cells

Cited 1 time

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HSP27 siRNA (Cat # 4392420 Ambion, CA) or negative control siRNA (Cat # 4611 Ambion, CA) was used to transfect 80–90% confluent CCD-18Co cells with Lipofectamine 2000 (Invitrogen, CA) at 100 nM according to the manufacturer’s instructions. Western blotting confirmed the silencing effect of siRNA on the expression levels of Hsp27 [12 (link)]. Transfected cells were then used for migration experiments.

Chu E., Saini S., Liu T, & Yoo J. (2016). Bradykinin Stimulates Protein Kinase D–mediated Colonic Myofibroblast Migration via COX-2 and Hsp27. The Journal of surgical research, 209, 191-198.

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HSP27 Silencing via siRNA Transfection

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80%–90% confluent cells were transfected with HSP27 siRNA (Cat # 4392420 Ambion, CA) or negative control siRNA (Cat # 4611 Ambion, CA) with Lipofectamine 2000 (Invitrogen, CA) at 100 nM according to the manufacturer’s instructions. Western blotting was then performed to test the silencing effect of siRNA on the expression levels of target proteins.

Saini S., Liu T, & Yoo J. (2016). TNF-α Stimulates Colonic Myofibroblast Migration via COX-2 and Hsp27. The Journal of surgical research, 204(1), 145-152.

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Cell Culture Reagents and Inhibitors

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Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), penicillin G potassium, streptomycin, fungizone, and glutamine were purchased from Invitrogen (Carlsbad, CA). TNF-α was purchased from R&D Systems (Minneapolis, MN). Hsp27 siRNA was purchased from Ambion (Carlsbad, CA). NS398 was purchased from Calbiochem (San Diego, CA). CID755673 was purchased from Sigma-Aldrich (St. Louis, MO).

Chu E., Saini S., Liu T, & Yoo J. (2016). Bradykinin Stimulates Protein Kinase D–mediated Colonic Myofibroblast Migration via COX-2 and Hsp27. The Journal of surgical research, 209, 191-198.

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