Ascorbic acid

Manufactured by Tokyo Chemical Industry

Sourced in Belgium

Ascorbic acid is a white crystalline compound that is commonly used in laboratory settings. It is a water-soluble vitamin, also known as vitamin C. Ascorbic acid is a reducing agent and an antioxidant, and it plays a vital role in various chemical reactions and analyses.

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5 protocols using ascorbic acid

Synthesis of Isoprene-Acrylonitrile Copolymer

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Example 7

In a glovebox in which the internal atmosphere had been replaced by nitrogen, a 30 mL glass reaction vessel was charged with 0.14 parts of copper(I) bromide, 5.4 parts of 1,10-phenanthroline, 1.76 parts of ascorbic acid (manufactured by Tokyo Chemical Industry Co., Ltd.) (reducing agent), 238.8 parts of acrylonitrile, 306.5 parts of isoprene, 0.27 parts of methyl 2-bromoisobutyrate, and 23.5 parts of mesitylene, and the mixture was stirred at 80° C. for 18 hours to effect a polymerization reaction.

After evaporating a volatile component from the reaction product under reduced pressure, the residue was dried to obtain an isoprene-acrylonitrile random copolymer.

The resulting isoprene-acrylonitrile random copolymer was analyzed, and it was found that the conversion rate of isoprene was 57%, the conversion rate of acrylonitrile was 55%, and the isoprene-acrylonitrile random copolymer had a weight average molecular weight (Mw) of 128,800, a number average molecular weight (Mn) of 76,200, and a molecular weight distribution (Mw/Mn) of 1.69. It was confirmed that a bromine atom was present at the terminal of the polymer chain.

US10351650B2. Method for producing conjugated diene polymer (2019-07-16). ZEON CORPORATION [JP]. Inventors: Fumiaki Bando [JP].

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Synthesis of Ruthenium-Copper Complexes

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Complex
Ru₂Ni(dpa)₄Cl₂ and Ru₂Cu(dpa)₄Cl₂ were synthesized following the
methods described before.^{28 (link)} Silver nitrate
(AgNO₃), CTAB, TBAP,
and gold(III) chloride trihydrate (HAuCl₄·3H₂O) were from Alfa Aesar, ACN, sodium bromide (NaBr), and sodium borohydride
(NaBH₄) from Sigma-Aldrich, CTAC from Tokyo Chemical Industry,
and sodium hydroxide (NaOH), potassium iodide (KI), ascorbic acid,
sodium citrate, and acetone from J.T.Baker. All chemicals were used
as received. Pure deionized water (Milli-Q Millipore, 18.2 MΩ/cm)
was used in all the preparations.

Wu B.H., Chung J.Y., Hung L.Y., Cheng M.C., Peng S.M, & Chen I.C. (2019). Facet-Dependent Reduction Reaction of Diruthenium Metal–String Complexes by Face-to-Face Linked Gold Nanocrystals. ACS Omega, 4(3), 5327-5334.

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Electrochemical Biosensor for Glucose and Interferents

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Pyrrole above 99% purity was obtained from Daejung-Korea. MWCNTs (Ca. ~10 nm in external diameter) synthesized via chemical vapor deposition (CVD) were purchased from Nanosolutions Co. Ltd., Korea. Glucose oxidase (GOx, EC 1.1.3.4, Type X-S 127 unit/mg) lyophilized powder, from Aspergillus niger, human blood serum (H4522), Nafion (Nf, 5 wt. % in lower aliphatic alcohol), L-cysteine (L-cys), and cholesterol were purchased from Sigma-Aldrich, Korea. β-D-Glucose and ascorbic acid (AA) were purchased from Tokyo Chemical Industry Co., Ltd. Dopamine (DA) and uric acid (UA) were obtained from Bioshop Canada Inc. Disodium hydrogen phosphate (Na₂HPO₄), monobasic potassium phosphate (KH₂PO₄), sodium chloride (NaCl), potassium chloride (KCl), sulphuric acid (H₂SO₄), nitric acid (HNO₃), and acetonitrile (CH₃CN) were obtained from Samchun Pure Co. Ltd., Korea. Phosphate buffer solution (PBS, 0.1 M, pH 7.4) was prepared in ultra-pure water purified by Millipore-Q system (18 MΩ cm). All chemicals and reagents were of analytical grade and were used as received without further purification.

Shrestha B.K., Ahmad R., Shrestha S., Park C.H, & Kim C.S. (2017). Globular Shaped Polypyrrole Doped Well-Dispersed Functionalized Multiwall Carbon Nanotubes/Nafion Composite for Enzymatic Glucose Biosensor Application. Scientific Reports, 7, 16191.

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Fibroblast Collagen Production with Ascorbic Acid

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100% confluent fibroblast layers on fluorodish were treated for 10 days with Ascorbic Acid (2-O-α-D-Glucopyranosyl-L-Ascorbic-Acid; Final concentration: 0.5 mM, TCI Europe, Zwijndrecht, Belgium). The presence of collagen was monitored using multiphoton analysis as described in the following.

Mazio C., Scognamiglio L.S., De Cegli R., Galietta L.J., Di Bernardo D., Casale C., Urciuolo F., Imparato G, & Netti P.A. (2020). Intrinsic Abnormalities of Cystic Fibrosis Airway Connective Tissue Revealed by an In Vitro 3D Stromal Model. Cells, 9(6), 1371.

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Antioxidant Potential Evaluation of Novel Compounds

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The novel compounds 1–9 were purchased from Vitas-M, 10 and 11 from Key Organics, 12–14 from ChemDiv, 15 from MolMall, 16 from MolPort, 17 from ChemBridge, and 18–21 from Sigma Aldrich, while the standard antioxidants aesculin, ascorbic acid, curcumin, scopoletin, Trolox and gallic acid were purchased from TCI Europe, resveratrol from ChromaDex, and quercetin from Sigma Aldrich. The structures of the novel compounds and standard antioxidants are presented in Fig 4.
2,2-diphenyl-1-picrylhydrazyl (DPPH), 2-deoxyribose, β-carotene, linoleic acid, lipoxidase from Glycine max, trypan blue solution, gentamicin, phorbol 12-myristate 13-acetate (PMA) and 2',7'-dichlorofluorescin diacetate (DCFH-DA) were all purchased from Sigma-Aldrich. Sterile dimethyl sulfoxide (DMSO) was obtained from WAK-Chemie Medical GmbH, fetal bovine serum (FBS), glutamine (GlutaMAX) and Dulbecco’s Phosphate-Buffered Saline (DPBS) from Gibco, Ficoll from Cedarlane, H₂O₂ from Merck, and RPMI 1640 medium from PAA Laboratories GmbH. All other chemicals (EDTA, FeCl₃, methanol, tween 20, chloroform, thiobarbituric acid, trichloroacetic acid, NaOH, KH₂PO₄, and K₂HPO₄) were of reagent grades and obtained from the local chemical vendors in Slovenia.

Martinčič R., Mravljak J., Švajger U., Perdih A., Anderluh M, & Novič M. (2015). In Silico Discovery of Novel Potent Antioxidants on the Basis of Pulvinic Acid and Coumarine Derivatives and Their Experimental Evaluation. PLoS ONE, 10(10), e0140602.

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