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One touch accu chek glucometer

Manufactured by Roche

The One‐Touch Accu‐Chek Glucometer is a portable device used to measure and monitor blood glucose levels. It is designed to provide accurate and reliable readings for individuals managing diabetes or other conditions requiring blood glucose monitoring.

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5 protocols using one touch accu chek glucometer

1

Metabolic Profiling of Rodent Model

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Bodyweight, food intake and fasting glucose level were recorded every month. Blood was collected from tail vein, and plasma was isolated by centrifugation. Serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), triglyceride (TG), total cholesterol (TC), low‐density lipoprotein cholesterol (LDL‐C) and HDL‐cholesterol (HDL‐C) levels were measured with an auto‐analyser (Cobas 6000 c501; Roche Diagnostics). Fasting blood glucose was measured with a One‐Touch Accu‐Chek Glucometer (Roche Diagnostics), and fasting serum insulin was determined using a rat‐specific insulin ELISA kit (Ultrasensitive Rat Insulin ELISA; Mercodia) following the kit instruction.
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2

Glucose and Insulin Tolerance in Mice

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For the glucose tolerance test (GTT), mice were fasted overnight followed by an intraperitoneal glucose injection (1 g/kg body weight). Blood glucose was measured by tail bleeding using the One-Touch AccuChek Glucometer (Roche) at indicated times. For the insulin tolerance test (ITT), mice without fast were injected intraperitoneally with insulin (Sigma-Aldrich) at 1.5 IU/kg body weight, and blood glucose was measured at indicated times.
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3

Rat Glucose and Insulin Tolerance Tests

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Intraperitoneal glucose tolerance tests (IPGTTs) were carried out on 16‐h fasted rats, and blood glucose levels were determined with a One‐Touch Accu‐Chek Glucometer (Roche Diagnostics) at 0, 5, 10, 30, 60 and 120 min by venous tail bleeding after a 2 g/kg body weight injection of glucose solution (Kelun). Intraperitoneal insulin tolerance tests (IPITTs) were performed after 6‐h fasting, blood glucose levels were measured as described above at 0, 5, 15, 30, 60 and 90 min after a 1.0 U/kg intraperitoneal injection of insulin solution (Wanbang).
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4

Glucose and Insulin Tolerance Tests

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Glucose tolerance tests were performed 4 weeks after HFD feeding by glucose gavage (1 g of glucose/kg body weight) after an overnight fast. For insulin tolerance tests, mice were anesthetized with pentobarbital 5 h after food removal and insulin (0.75 unit insulin/kg body weight) was injected intraperitoneally. Blood samples were taken from the tail vein at the indicated time points. Blood glucose was determined using the One-Touch Accu-Chek Glucometer (Roche).
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5

Glucose, Insulin, and Pyruvate Tolerance Tests

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Glucose tolerance tests were performed by oral administration of glucose (1 g D-glucose/kg body weight) after overnight fasting. Insulin tolerance tests were performed by intraperitoneal (ip) injection of human insulin (0.75 unit insulin/kg body wt, Actrapid Penfill, NovoNordisk) 5 h after food removal. Pyruvate tolerance tests were performed by ip injection of sodium salt pyruvate (2 g/kg body weight) after an overnight fast. Blood was collected from the tail vein in a heparinized capillary tube and glucose concentration was determined using the One-Touch AccuChek Glucometer (Roche).
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