Advanced dmem f 12 culture medium

Manufactured by Thermo Fisher Scientific

Sourced in Germany, Denmark

Advanced® DMEM/F-12 culture medium is a cell culture medium formulation designed to support the growth and maintenance of a wide range of cell types. It is a defined, serum-free medium that provides the necessary nutrients and growth factors for cell culture applications.

Automatically generated - may contain errors

Lab products found in correlation

Spelling variants of this product

DMEM medium (4294 citations) Advanced DMEM (195 citations) DMEM culture medium (193 citations) Advanced DMEM medium (24 citations) F-12 culture medium (5 citations) Culture mediums (3 citations) Advanced DMEM/F-12 (DMEM, (2 citations) DMEM/F 12 culture (2 citations)

7 protocols using advanced dmem f 12 culture medium

Host Cell-free Culture of Porcine Malaria Parasites

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

To start the host cell-free culture, free merozoites were obtained from monolayer culture supernatant of intestinal porcine epithelial cells 6 days after infection with sporozoites (Feix et al., 2020 (link)). Merozoites were washed with phosphate-buffered saline (PBS; Gibco, Thermo Fisher Science, Waltham, USA) and purified by density gradient centrifugation on a Percoll^® (GE Healthcare, Uppsala, Sweden) gradient (60, 40 and 20%, topped up with the merozoite suspension in PBS) at 600 × g for 10 min at 20°C in a MegaStar 3.0R swing bucket centrifuge (VWR International, Leuven, Belgium). Both acceleration and deceleration were at the lowest possible setting. Purified merozoites were transferred to fresh Advanced^® DMEM/F-12 culture medium (Gibco) supplemented with 5% fetal calf serum (Gibco) and penicillin/streptomycin plus l-glutamine 100x (Gibco) onto a new uncoated ibidi 8-well ibiTreat^®μ-slide (ibidi, Gräfelfing, Germany) at a concentration of 1.2 × 10⁵ merozoites per mL medium and were incubated at 40°C under 5% CO₂. The development of parasite stages was monitored daily, and stages were harvested at 3 and 4 dpt (days post transfer), pelleted and stored at −20°C.

Feix A.S., Cruz-Bustos T., Ruttkowski B., Mötz M., Rümenapf T, & Joachim A. (2021). Progression of asexual to sexual stages of Cystoisospora suis in a host cell-free environment as a model for Coccidia. Parasitology, 148(12), 1475-1481.

+ Open protocol

+ Expand

Mandibular Molar Placode Culture

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

All experimental procedures were performed following the requirements and approval of Pontificia Universidad Católica de Chile and the King’s College London Ethics Committees. Mouse embryos were collected at E14.5 to perform cultures. Experiments were performed in wild type CD1 and/or C57BL/6J according to the availability of the strains. Figures show CD1 data. Supplementary Figure S1 show C57BL/6J data. For cultures, we used the air-liquid interface method: dissected mandibular molar placodes were placed on top of transparent nucleopore filters (VWR) supported by metal grids at the surface of Advanced DMEM/F12 culture medium (Gibco®), supplemented with 1% penicillin/streptomycin (Gibco®) and 1% GlutaMAX®. These cultures were incubated at 37°C/5% CO₂, and the medium was changed three times per week (Alfaqeeh et al., 2013 (link); Gaete and Tucker, 2013 (link); Gaete et al., 2015 (link)). The cultures were treated with 200 μM 4-methylumbelliferone (4-Mu; a chemical inhibitor for HA synthesis, Sigma-Aldrich M1381) from a of 200 mM stock solution in DMSO, while DMSO was added to control cultures. This concentration of 4-Mu has previously been shown to cause inhibition of tail regeneration in Xenopus laevis tadpoles (Contreras et al., 2009 (link)) and was chosen after a titration in our system. Experimental and control pairs were obtained using contralateral molar regions.

Sánchez N., González-Ramírez M.C., Contreras E.G., Ubilla A., Li J., Valencia A., Wilson A., Green J.B., Tucker A.S, & Gaete M. (2020). Balance Between Tooth Size and Tooth Number Is Controlled by Hyaluronan. Frontiers in Physiology, 11, 996.

+ Open protocol

+ Expand

APC Mutant Organoid Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Organoids were produced from WT and APC^min/+ mice as previously described [13 , 36 ]. For adenoma and APC^min/+ organoids, adenomas and normal adjacent tissue were separated and processed accordingly. All organoids were grown in Advanced DMEM/F12 culture medium (Gibco) supplemented with Noggin, EGF, bFGF (Peprotech, 1:1000), R-spondin1 (Peprotech, 1:500) and B27 (Gibco, 1:50). For staining, organoids were fixed in 4 % paraformaldehyde and incubated overnight with the primary antibody (IFITM2/3 - 1:200, Abcam). Secondary antibody was Alexa fluor-647 donkey anti-rabbit (Molecular Probes, 1:1000). Hoechst (1 μg/ml, Molecular Probes) was used to stain nuclei.

Aran D., Lasry A., Zinger A., Biton M., Pikarsky E., Hellman A., Butte A.J, & Ben-Neriah Y. (2016). Widespread parainflammation in human cancer. Genome Biology, 17, 145.

+ Open protocol

+ Expand

Exploring the Independent Life Cycle of C. suis

Check if the same lab product or an alternative is used in the 5 most similar protocols

To check whether the C. suis life cycle can exclusively occur outside of a host cell, 5 × 10³ sporozoites were released into fresh Advanced^® DMEM/F-12 culture medium (Gibco) supplemented with 5% fetal calf serum (Gibco) and penicillin/streptomycin plus l-glutamine 100x (Gibco) onto a new uncoated ibidi 8-well ibiTreat^®μ-slide (ibidi, Gräfelfing, Germany) and were incubated at 40°C under 5% CO₂. The development of parasite stages was monitored daily.

+ Open protocol

+ Expand

Preparation and Characterization of DCVC

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cell and tissue culture reagents including RPMI 1640 culture medium, advanced DMEM/F-12 culture medium, F12-K Nutrient Mixture Kaighn’s Modification culture medium, 10,000 U/mL penicillin and 10,000 μg/mL streptomycin (P/S) mixed solution, Antibiotic-Antimycotic (Anti-Anti) mixed solution, and fetal bovine serum (FBS) were purchased from Gibco, a division of Thermo Fisher Scientific (Waltham, MA, USA). Phosphate buffered saline (PBS) and 0.25% trypsin were purchased from Invitrogen Life Technologies (Carlsbad, CA, USA). TCA and DCA were purchased from MilliporeSigma (St. Louis, MO, USA). TCVC was purchased from Toronto Research Chemicals (North York, ON, Canada). DCVC was synthesized as a powder by the University of Michigan Medicinal Chemistry Core as previously described[40 ]. High-performance liquid chromatography analysis was used to determine purity (98.7%). A stock solution of 1 mM DCVC was prepared by dissolving DCVC in phosphate buffered saline and stored in small aliquots at −20°C to minimize freeze/thaw cycles. The chemical purity of the DCVC stock solution was confirmed nuclear magnetic resonance (NMR).

Elkin E.R., Su A.L., Kilburn B.A., Bakulski K.M., Armant D.R, & Loch-Caruso R. (2022). Toxicity assessments of selected trichloroethylene and perchloroethylene metabolites in three in vitro human placental models. Reproductive toxicology (Elmsford, N.Y.), 109, 109-120.

+ Open protocol

+ Expand

Breast Cancer Tissue Organoid Isolation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Finely chopped excised breast cancer tissue and matched normal breast tissue incubated for 4 hours on a shaking table (set at 60 rpm) at 37 °C in advanced DMEM/F12 culture medium (Life Technologies, Nærum, Denmark) aerated with 5% CO₂/balance air and supplemented with 10% fetal bovine serum (Biochrom, Cambridge, UK), 1% Glutamax (Gibco, Invitrogen, Roskilde, Denmark), and 450 IU/mL collagenase type 3 (Worthington Biochemical Corporation, Lakewood, NJ, USA). Organoids are 100–150 µm in diameter and consist primarily of cytokeratin-19-positive cancer cells with smaller numbers of other cell types (e.g., myofibroblasts and tumor-associated macrophages) [8 (link),9 (link)]. From the partially digested breast tissue, organoids were isolated by sedimentation for 20 min and used directly for experiments without culture in order to avoid changes in expression profile or phenotype.

Voss N.C., Dreyer T., Henningsen M.B., Vahl P., Honoré B, & Boedtkjer E. (2020). Targeting the Acidic Tumor Microenvironment: Unexpected Pro-Neoplastic Effects of Oral NaHCO3 Therapy in Murine Breast Tissue. Cancers, 12(4), 891.

+ Open protocol

+ Expand

Isolation of Breast Cancer Organoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Biopsies of breast cancer tissue and matched normal breast tissue were finely chopped in phosphate-buffered saline (PBS) before transfer to advanced DMEM/F12 culture medium (Life Technologies, Denmark) supplemented with 10% fetal bovine serum (Biochrom, Germany), 1% Glutamax (Gibco, Invitrogen, Denmark), and 450 IU/mL collagenase type 3 (Worthington Biochemicals, USA). The tissue was incubated in this solution for 4 h (mouse) or overnight (human) on a shaking table at 60 rpm in a 37 °C atmosphere of 5% CO₂/balance air. At the end of tissue digestion, organoids around 150 µm in diameter were sedimented by gravitational forces for 20 min [7 (link), 25 (link)]. We immediately investigated the freshly isolated organoids experimentally in order to avoid culture-induced phenotypical changes.

Lee S., Toft N.J., Axelsen T.V., Espejo M.S., Pedersen T.M., Mele M., Pedersen H.L., Balling E., Johansen T., Burton M., Thomassen M., Vahl P., Christiansen P, & Boedtkjer E. (2023). Carbonic anhydrases reduce the acidity of the tumor microenvironment, promote immune infiltration, decelerate tumor growth, and improve survival in ErbB2/HER2-enriched breast cancer. Breast Cancer Research : BCR, 25, 46.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!