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Anti glp

Manufactured by R&D Systems
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Sourced in United States, Japan

Anti-GLP is a laboratory reagent used in research applications. It functions as an antibody that specifically binds to the glucagon-like peptide (GLP) protein. The primary role of Anti-GLP is to facilitate the detection and quantification of GLP in biological samples during experimental procedures.

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Lab products found in correlation

Anti g9a, by R&D Systems (3 mentions) Anti h3k9me2, by Abcam (2 mentions) Anti plzf, by Santa Cruz Biotechnology (1 mentions) Anti γh2ax, by Abcam (1 mentions) Anti nanog, by Cosmo Bio (1 mentions) Anti h3k9me2, by Merck Group (1 mentions) Anti gfp, by Nacalai Tesque (1 mentions) Anti ap2γ, by Santa Cruz Biotechnology (1 mentions) Anti ki67, by BD (1 mentions) Anti dnmt3b, by Abcam (1 mentions)

Close spelling variants of this product

Anti-ETMT1/GLP mouse monoclonal

3 protocols using anti glp

1

Immunofluorescence Characterization of L1 and IAP

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Rabbit polyclonal L1 ORF1 antisera were made through immunization of rabbits with recombinant ORF1 protein. The following antibodies were used at the indicated dilutions for IF: anti-G9a (1:50) (A. Tarakhovsky, The Rockefeller University, New York, NY, USA), anti-ORF1 L1 (1:500), mouse monoclonal anti-GLP (R&D systems, Minneapolis, MN, USA, PP-B0422-00) (1:100), anti-IAP Gag (1:500) (B. Cullen, Duke University, Durham, NC, USA), mouse monoclonal anti-γH2AX (Abcam, Cambridge, UK ab26350) (1:500), mouse monoclonal anti-H3K9me2 (Abcam, Cambridge, UK ab1220) (1:100) and rabbit polyclonal anti-Plzf (Santa Cruz, Dallas, TX, USA sc-22839) (1:100). Immunofluorescence and histology were performed as described [3 (link)]. Quantification of L1 Orf1 and IAP signal from at least 20 to 40 cells was performed using Fiji software (http://fiji.sc/Fiji).
Di Giacomo M., Comazzetto S., Sampath S.C., Sampath S.C, & O’Carroll D. (2014). G9a co-suppresses LINE1 elements in spermatogonia. Epigenetics & Chromatin, 7, 24.
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2

Embryonic Chromatin Modification Profiling

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Embryos were dissected from the decidua and Reichert’s membrane and treated as previously described (Nichols et al., 2009 (link)). Primary antibodies used are as follows: anti-H3K9me2 (Millipore, CA, 17–648), anti-H3K9me2 (Abcam, UK, ab1220), anti-GFP (Nacalai tesque, Japan, GF090R), anti-G9a (R&D Systems, MN, A8620A), anti-GLP (R&D Systems, MN, PP-B0422-00), anti-cleaved Caspase 3 (Abcam, UK, ab32042), anti-Ki67 (BD Bioscience, NJ, 550609), anti-NANOG (Cosmobio, Japan, REC-RCAB002P-F), anti-AP2γ (Santa Cruz, CA, sc-9877). All imaging was performed using SP5 or SP7 confocal microscope (Leica, Germany).
Zylicz J.J., Dietmann S., Günesdogan U., Hackett J.A., Cougot D., Lee C, & Surani M.A. (2015). Chromatin dynamics and the role of G9a in gene regulation and enhancer silencing during early mouse development. eLife, 4, e09571.
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3

Epigenetic Regulation in Cardiac Fibrosis

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The following primary antibodies were used for immunostaining: anti-G9a (Cat# PP-A8620A-00, R&D Systems), anti-GLP (Cat# PP-B0422–00, R&D Systems), anti-DNMT3a (H-295, Cat# sc20703, Santa Cruz Biotechnology), anti-DNMT3b (Cat# ab16049, Abcam). The immunofluorescence staining of G9a or Dnmt3a by measuring the intensity of nuclear fluorescence signals and background using Image J. The corrected intensity (intensity — background) of staining was used. At least four measurements were made for each heart. N represents the number of animals used within each group. To quantitate cardiac interstitial fibrosis, the perivascular fibrotic areas were excluded. The blue channel (fibrotic) and red channel (non-fibrotic) were separated by Photoshop and quantitated by Image J. The ratios of (area of blue)/(area of blue and red) were used to evaluate the fibrosis. At least four measurements were made for each heart. N represents the animals used within a group.
Han P., Li W., Yang J., Shang C., Lin C.H., Cheng W., Hang C.T., Cheng H.L., Chen C.H., Wong J., Xiong Y., Zhao M., Drakos S.G., Ghetti A., Li D.Y., Bernstein D., Chen H.S., Quertermous T, & Chang C.P. (2016). Epigenetic response to environmental stress: Assembly of BRG1–G9a/GLP–DNMT3 repressive chromatin complex on Myh6 promoter in pathologically stressed hearts,. Biochimica et biophysica acta, 1863(7 Pt B), 1772-1781.
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