Anti mouse cd40 clone hm40 3

Manufactured by BD

Anti-mouse CD40 (clone HM40-3) is a lab equipment product used for the detection and analysis of CD40 expression on mouse cells. It is a monoclonal antibody that specifically binds to the mouse CD40 antigen.

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Lab products found in correlation

Lipopolysaccharides (lps), by Merck Group (1 mentions) Interleukin 4 (il 4), by R&D Systems (1 mentions) Trizol reagent, by Thermo Fisher Scientific (1 mentions) Macs b cell isolation kit, by Miltenyi Biotec (1 mentions)

Close spelling variants of this product

Anti-CD40 (48 citations) Anti-mouse CD40 (9 citations) Anti-CD40 (hm40-3 clone; (6 citations) HM40–3 (6 citations) Anti-CD40 (HM40–3; (5 citations) Clone HM40-3 (3 citations) Mouse anti (2 citations)

2 protocols using anti mouse cd40 clone hm40 3

B Cell Stimulation Protocol

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Purified B cells from the indicated genotypes were cultured in the presence of: 1 µg/ml anti–mouse CD40 (clone HM40-3; BD), 20 µg/ml LPS (Sigma-Aldrich), 20 ng/ml IL-4 (R&D Systems), and 16.25 µg/ml anti–mouse IgM (Jackson ImmunoResearch Laboratories, Inc.). Cell density in CD40+IgM stimulation experiments was 1.5 × 10⁶ cells/ml. In all other experiments, cells were cultured at a density of 10⁶ cells/ml.

Heise N., De Silva N.S., Silva K., Carette A., Simonetti G., Pasparakis M, & Klein U. (2014). Germinal center B cell maintenance and differentiation are controlled by distinct NF-κB transcription factor subunits. The Journal of Experimental Medicine, 211(10), 2103-2118.

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Isolation and Stimulation of Mouse Splenic B Cells

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Single cell suspensions of mouse spleen were subjected to hypotonic lysis and B cells were purified by depletion of magnetically labeled non-B cells using the MACS B-cell isolation kit (Miltenyi Biotec). Purified B cells from the indicated genotypes were cultured in the presence of: 1 μg/ml anti-mouse CD40 (clone HM40-3; BD Pharmingen) or 25 ng/ml BAFF (R&D Systems). Cell density in CD40 and BAFF stimulation experiments was 1.5 × 10⁶ cells/ml. For the RNA-seq analysis, cell pellets were lysed with TRIzol reagent (Life Technologies) for RNA isolation. For Western blot analysis, purified B cells were washed once with PBS and subjected to NP40-based lysis.

De Silva N.S., Silva K., Anderson M.M., Bhagat G, & Klein U. (2016). Impairment of mature B-cell maintenance upon combined deletion of the alternative NF-κB transcription factors RELB and NF-κB2 in B cells. Journal of immunology (Baltimore, Md. : 1950), 196(6), 2591-2601.

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