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Ab reagent

Manufactured by Vector Laboratories
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AB reagent is a laboratory product designed for use in various applications. It serves as a core component in specific experimental procedures, facilitating the identification or detection of target analytes. The product's function is to provide a reliable and consistent means of achieving the desired results within the intended experimental protocols.

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Lab products found in correlation

Biotinylated goat anti rabbit antibody, by Agilent Technologies (2 mentions) Fluorescein tyramide signal amplification systems, by PerkinElmer (2 mentions) Goat anti mouse 1 200 alexa 568 antibody, by Thermo Fisher Scientific (2 mentions) Dmi 6000 time lapse microscope, by Leica (2 mentions) Dab reagent, by Vector Laboratories (1 mentions) Eclipse 80i microscope, by Nikon (1 mentions) Triton x 100, by Merck Group (1 mentions) Pannoramic 250 flash 3, by 3DHISTECH (1 mentions) Optical light microscope, by Olympus (1 mentions) Sc 805, by Santa Cruz Biotechnology (1 mentions) Axioplan 2 microscope, by Zeiss (1 mentions) Triton 100, by Merck Group (1 mentions) Adi spa 896 f, by Enzo Life Sciences (1 mentions) Donkey anti rabbit hrpconjugate secondary antibody, by Santa Cruz Biotechnology (1 mentions) Cyanine 3 tyramide, by PerkinElmer (1 mentions) Superblock blocking buffer, by Thermo Fisher Scientific (1 mentions) Tcs sp5 confocal microscope, by Leica (1 mentions)

5 protocols using ab reagent

1

Immunohistochemical Analysis of Ubc13 in Breast Tissue

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Breast tumors and organs from different groups were excised and fixed in 10% PFA and in 70% ethanol. Tissue paraffin embedding, sectioning and H & E staining were performed by the Histology Core facility, Department of Pathology, UMMC. The stained slides were evaluated by pathologist (KVA) who was blinded to the treatment. For immunohistochemistry, human benign and breast biopsy specimens were obtained from pathology repository. Paraffin-embedded specimens were deparaffinized in xylene, subjected to heat-mediated antigen-retrieval in 10 mM sodium citrate (pH 6.0), permeabilized in 0.2% Triton X-100 (Sigma) and blocked in 5% donkey sera. Ubc13 was detected using anti-Ubc13 antibody (Thermofischer) (1:100) and an HRP-conjugated donkey anti-rabbit secondary (1:250, Abcam), amplified with AB reagent (Vectastain) and detected using DAB reagent (Vector Laboratories). Images were acquired using a Nikon Eclipse 80i microscope.
Vallabhaneni K.C., Penfornis P., Xing F., Hassler Y., Adams K.V., Mo Y.Y., Watabe K, & Pochampally R. (2017). Stromal cell extracellular vesicular cargo mediated regulation of breast cancer cell metastasis via ubiquitin conjugating enzyme E2 N pathway. Oncotarget, 8(66), 109861-109876.
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2

Histological Evaluation of Bone Samples

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All samples were decalcified in 15% ethylenediaminetetraacetic acid (EDTA, pH 7.2) and refreshed every 3 days for 4 weeks. After dehydrated using a tissue processor, samples were embedded in paraffin, and cut into serial 5-μm sections (Leica, Germany) for staining. The pathological sections of each group were evaluated by Hematoxylin and eosin staining (H&E, Beyotime, China), Masson's trichrome staining, and images were captured with a light optical microscope (Olympus, Japan).
To perform immunohistochemistry (IHC) staining, deparaffinized samples were stained with ALP, Runx2, OCN, VEGF and CD31. The primary antibodies used in the present study were listed in Table S2. The secondary antibodies were treated for 1 h at room temperature. After secondary antibody treatment, sections were washed three times with PBS, and then labeled using AB reagent (Vector, USA) for 30 min to couple with DAB. Tissue sections were counterstained with hematoxylin and then mounted using mounting medium. Stained tissue images were obtained by digital pathology scanner (Pannoramic 250 FLASH III, 3D Histech, Hungary). The expression of IHC stained sections were measured using Image J program (IHC profiler).
Kang Y., Xu C., Meng L., Dong X., Qi M, & Jiang D. (2022). Exosome-functionalized magnesium-organic framework-based scaffolds with osteogenic, angiogenic and anti-inflammatory properties for accelerated bone regeneration. Bioactive Materials, 18, 26-41.
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3

Immunohistochemical Analysis of Lung Tissue

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Sections from paraffin-embedded mouse and human lung were deparaffinized
in xylene, subjected to heat-mediated antigen-retrieval in 1 mM EDTA with
0.05% Tween 20 (pH 8.0), endogenous peroxide activity was quenched in
2.5% hydrogen peroxide solution in methanol, slides were permeabilized
in 0.1% Triton-100 (Sigma) and blocked in SuperBlock Blocking Buffer
(Thermo Scientific). For immunohistochemistry, sections were stained with rabbit
anti-HO1 (1:100, ADI-SPA-896-F, Enzo Life Sciences), rabbit anti-HA (1:50,
sc-805 Santa Cruz), or mouse anti-CD68 (1:100, ab955 Abcam). HRP-conjugated
secondary antibodies were obtained from Jackson Immunochemicals and used at
1:250. Staining was amplified with AB reagent (Vectastain) and detected using
DAB reagent (Thermo Scientific). Images were acquired using a Zeiss Axioplan 2
microscope. For immunofluorescence, HO1 was identified using rabbit anti-HO1
(1:100) and a donkey anti-rabbit-HRP conjugate secondary antibody (1:500, Santa
Cruz) followed by amplification with Cyanine 3 tyramide (1:100, Perkin Elmer).
Mtb was identified using guinea pig anti-Mtb (1:25, NR-13818, NR-13823 BEI) and
an Alexa 488 conjugated donkey anti-guinea pig secondary antibody (1:100,
706-545-148 Jackson Immunochemicals). All commercial antibodies were prepared
without Freund’s adjuvant. Images were acquired using a Leica TCS SP5
confocal microscope.
Scharn C.R., Collins A.C., Nair V.R., Stamm C.E., Marciano D.K., Graviss E.A, & Shiloh M.U. (2016). Heme oxygenase-1 regulates inflammation and mycobacterial survival in human macrophages during M. tuberculosis infection. Journal of immunology (Baltimore, Md. : 1950), 196(11), 4641-4649.
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4

Multiparametric Analysis of Proliferation

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Liver tissue was fixed in ice-cold 10% formalin and paraffin embedded tissue sections (5μm) were used for IHC. Antigen retrieval was performed in 1×citrate buffer in microwave pressure cooker. We used 1/200 dilution of BrdU plus Phospho-histone H3 antibodies (phospho-histone H3:Cell signalling #9701; BrdU BD). Phospho-histone H3 (P-histone H3) was detected using a biotinylated goat Anti-Rabbit antibody (Dako) and the AB reagents from Vectastain (Vector labs). The fluorescent signal was provided using the Fluorescein Tyramide Signal Amplification (TSA) Systems (Perkin elmer). BrdU antibody was detected using a goat anti mouse 1/200 Alexa 568 antibody (Molecular Probes life technologies). Cells were counterstained with Dapi (500ng/ml) and slides were mounted with mowiol. Pictures were taken using a Leica DMI6000 time lapse microscope.
Méniel V., Megges M., Young M.A., Cole A., Sansom O.J, & Clarke A.R. (2014). Apc and p53 interaction in DNA damage and genomic instability in hepatocytes. Oncogene, 34(31), 4118-4129.
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5

Multiparametric Analysis of Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols
Liver tissue was fixed in ice-cold 10% formalin and paraffin embedded tissue sections (5μm) were used for IHC. Antigen retrieval was performed in 1×citrate buffer in microwave pressure cooker. We used 1/200 dilution of BrdU plus Phospho-histone H3 antibodies (phospho-histone H3:Cell signalling #9701; BrdU BD). Phospho-histone H3 (P-histone H3) was detected using a biotinylated goat Anti-Rabbit antibody (Dako) and the AB reagents from Vectastain (Vector labs). The fluorescent signal was provided using the Fluorescein Tyramide Signal Amplification (TSA) Systems (Perkin elmer). BrdU antibody was detected using a goat anti mouse 1/200 Alexa 568 antibody (Molecular Probes life technologies). Cells were counterstained with Dapi (500ng/ml) and slides were mounted with mowiol. Pictures were taken using a Leica DMI6000 time lapse microscope.
Méniel V., Megges M., Young M.A., Cole A., Sansom O.J, & Clarke A.R. (2014). Apc and p53 interaction in DNA damage and genomic instability in hepatocytes. Oncogene, 34(31), 4118-4129.
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