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Aiib2

Manufactured by Developmental Studies Hybridoma Bank
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Sourced in United States

AIIB2 is a laboratory instrument designed for the detection and quantification of specific proteins or other biomolecules. It utilizes a technique called Enzyme-Linked Immunosorbent Assay (ELISA) to perform these analyses. The core function of AIIB2 is to provide accurate and reliable measurement of target analytes in a variety of sample types.

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Lab products found in correlation

Biig2, by Developmental Studies Hybridoma Bank (2 mentions) Ab52971, by Abcam (1 mentions) Ab33186, by Abcam (1 mentions) Ab109185, by Abcam (1 mentions) Collagenase type 1, by Solarbio (1 mentions) Ab1416, by Abcam (1 mentions) Culture media, by Thermo Fisher Scientific (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Trizol, by Thermo Fisher Scientific (1 mentions) Sirna, by GenePharma (1 mentions) Collagen gel, by Nitta Gelatin (1 mentions) Collagen 4 coated coverslips, by Merck Group (1 mentions)

4 protocols using aiib2

1

Integrin and Rac1 Regulation in Cell-ECM Interactions

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Antibodies used include anti-β1 integrin (ab52971, Abcam), anti-Rac1 (ab33186, Abcam), anti-E-cad (ab1416, Abcam), and anti-MUC-1 (ab109185, Abcam). Other reagents included β1 integrin inhibitor AIIB2 (Developmental Studies Hybridoma Bank, United States), type I collagenase (Solarbio, China), collagen gel (Nitta Gelatin, Japan), culture media (Gibco), and Lipofectamine 2000 and TRIzol (Invitrogen). siRNAs were synthesized by GenePharma, China, and the sequences were as follows: siRNA-β1 integrin: forward, 5′-GUU UAA UGU CUG GUG CUU TT-3′; reverse, 5′-AAG CAC CAG ACA UUA AAC TT-3′; siRNA-Rac1: forward, 5′-UAA AGA CAC GAU CGA GAA AUU-3′; reverse, 5′-UUU CUC GAU CGU GUC UUU AUU-3′; and siRNA-ctrl: forward, 5′-UUC UCC GAA CGU GUC ACG UTT-3′; reverse, 5′-ACGUGACACGUUCGGAGAATT-3′.
Liu B., Zheng X., Meng F., Han Y., Song Y., Liu F., Li S., Zhang L., Gu F., Zhang X, & Fu L. (2017). Overexpression of β1 integrin contributes to polarity reversal and a poor prognosis of breast invasive micropapillary carcinoma. Oncotarget, 9(4), 4338-4353.
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2

Fn-mediated Sprouting Assays with Integrin Blocking

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Sprouting assays were performed as previously described. For Fn9*10 gels, HUVEC beads were suspended at a concentration of 500 beads/ml in 2 mg/ml fibrinogen (Fib1 or Fib3), 1 U/ml factor XIII, 0.04 U/ml aprotinin and 2 μM (high dosage) or 0.267 μM (low dosage) Fn9*10 at a pH of 7.4 with or without 5μg/ml of β1 integrin blocking antibody (AIIB2, Developmental Studies Hybridoma Bank) or α5 integrin blocking antibody (BIIG2, Developmental Studies Hybridoma Bank). The blocking antibody (5μg/ml) in fresh fibronectin-free EGM-2 medium was replenished every day.
For Fn9(4G)10 gels, HUVEC beads were suspended at a concentration of 500 beads/ml in 2 mg/ml fibrinogen (Fib1 or Fib3), 1 U/ml factor XIII, 0.04 U/ml aprotinin and 2 μM (high dosage) or 0.239 μM (low dosage) Fn9(4G)10 at a pH of 7.4 with or without 5μg/ml of β3 integrin blocking antibody (9H5, Developmental Studies Hybridoma Bank) or αv integrin blocking antibody (P3G8, Developmental Studies Hybridoma Bank). The blocking antibody (5μg/ml) in fresh fibronectin-free EGM-2 medium was replenished every day.
Li S., Nih L.R., Bachman H., Fei P., Li Y., Nam E., Dimatteo R., Carmichael S.T., Barker T.H, & Segura T. (2017). Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability. Nature materials, 16(9), 953-961.
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3

Integrin Antibody Modulation of Cell Adhesion

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Cells were incubated in media containing 10%FBS and the respective β1-integrin antibody: inhibiting (AIIB2:20μg/ml (Developmental Studies Hybridoma Bank), (P4C10: 10μg/ml (Millipore)) and activating (TS2/16 and 12G10: 10μg/ml) (Santacruz), for 1 hour at 4°C. Cells were then plated on collagen IV-coated coverslips (Sigma) and incubated in the antibody at 37°C, for 24 hours.
Dey-Guha I., Alves C.P., Yeh A.C., S.a.l.o.n.y., Sole X., Darp R, & Ramaswamy S. (2015). A MECHANISM FOR ASYMMETRIC CELL DIVISION RESULTING IN PROLIFERATIVE ASYNCHRONICITY. Molecular cancer research : MCR, 13(2), 223-230.
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4

Fn-mediated Sprouting Assays with Integrin Blocking

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Sprouting assays were performed as previously described. For Fn9*10 gels, HUVEC beads were suspended at a concentration of 500 beads/ml in 2 mg/ml fibrinogen (Fib1 or Fib3), 1 U/ml factor XIII, 0.04 U/ml aprotinin and 2 μM (high dosage) or 0.267 μM (low dosage) Fn9*10 at a pH of 7.4 with or without 5μg/ml of β1 integrin blocking antibody (AIIB2, Developmental Studies Hybridoma Bank) or α5 integrin blocking antibody (BIIG2, Developmental Studies Hybridoma Bank). The blocking antibody (5μg/ml) in fresh fibronectin-free EGM-2 medium was replenished every day.
For Fn9(4G)10 gels, HUVEC beads were suspended at a concentration of 500 beads/ml in 2 mg/ml fibrinogen (Fib1 or Fib3), 1 U/ml factor XIII, 0.04 U/ml aprotinin and 2 μM (high dosage) or 0.239 μM (low dosage) Fn9(4G)10 at a pH of 7.4 with or without 5μg/ml of β3 integrin blocking antibody (9H5, Developmental Studies Hybridoma Bank) or αv integrin blocking antibody (P3G8, Developmental Studies Hybridoma Bank). The blocking antibody (5μg/ml) in fresh fibronectin-free EGM-2 medium was replenished every day.
Li S., Nih L.R., Bachman H., Fei P., Li Y., Nam E., Dimatteo R., Carmichael S.T., Barker T.H, & Segura T. (2017). Hydrogels with precisely controlled integrin activation dictate vascular patterning and permeability. Nature materials, 16(9), 953-961.
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