Nanodrop nd 1000 uv

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Nanodrop ND-1000 UV is a spectrophotometer used to measure the concentration and purity of nucleic acid and protein samples. It requires only 1-2 microliters of sample to perform the analysis.

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Lab products found in correlation

Abi 7900ht sds, by Thermo Fisher Scientific (1 mentions) Geneamp pcr system 9700, by Thermo Fisher Scientific (1 mentions) 2100 bioanalyzer instrument, by Agilent Technologies (1 mentions) Ferrous sulfate heptahydrate feso4 7h2o, by Merck Group (1 mentions) Nanodrop, by Merck Group (1 mentions) Pda medium, by BD (1 mentions) Bc0090 50 t 48s, by Solarbio (1 mentions) Rnaprep pure plant kit, by Tiangen Biotech (1 mentions)

3 protocols using nanodrop nd 1000 uv

RNA Yield and Integrity Analysis

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Total RNA yield was determined
by absorbance
measurement (A260) on the Nanodrop ND-1000 UV spectrometer (Thermo
Scientific, Wilmington, DE). All blood samples produced RNA concentrations
above the lower detection limit of 2 ng/μL of the NanoDrop instrument.
RNA integrity was determined by the Agilent Bioanalyzer utilizing
RNA 6000 chips (Agilent, Santa Clara, CA). All PCR runs were performed
using the ABI 7900HT SDS (Applied Biosystems, Foster City, CA), and
RT reactions were performed using the GeneAmp PCR System 9700 (Applied
Biosystems). Capillary gel electrophoresis was done using the Bioanalyzer
2100 instrument (Agilent).

Kelnar K., Peltier H.J., Leatherbury N., Stoudemire J, & Bader A.G. (2014). Quantification of Therapeutic miRNA Mimics in Whole Blood from Nonhuman Primates. Analytical Chemistry, 86(3), 1534-1542.

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Aspergillus flavus Cultivation and Antioxidant Assays

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A. flavus NRRL3357 was obtained from Sun Yat-sen University (Guangdong, China) and cultivated on potato dextrose agar (PDA) medium (Becton, Dickinson and Company, USA) at 30 °C. The spore suspension was harvested with 0.05 % Triton X-100, and the spore number was counted using a hemocytometer under a microscope. DNA was extracted using a Biospin fungus genomic DNA extraction kit from cultured A. flavus hyphae, and a Nanodrop (Nanodrop ND-1000 UV, Thermo Scientific) was used to measure the purity of DNA using an absorption ratio of 260 to A280, the DNA concentration was measured by Nanodrop and adjusted to a final concentration of 2 × 10³ ng/μL. Ferrous sulfate heptahydrate (FeSO₄·7H₂O) was purchased from Sigma. Chemical kits used for testing activity of CAT (BC0200-50 T/48S), POD (BC0090-50 T/48S) and SOD (BC0170-50 T/24S) were purchased from Solarbio (Beijing Solarbio Science & Technology Co., ltd.). All other chemical agents were analytical grade.

Yao L., Zhang T., Peng S., Xu D., Liu Z., Li H., Hu L, & Mo H. (2022). Fe2+ protects postharvest pitaya (Hylocereus undulatus britt) from Aspergillus. flavus infection by directly binding its genomic DNA. Food Chemistry: Molecular Sciences, 5, 100135.

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Extracting RNA from Dimorphic Saltwort Seeds

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Total RNA was isolated from 0.1 g cotyledons of S. aralocaspica according to the manufacturer's instructions in the RNAprep Pure Plant Kit (DP432; Tiangen, Beijing, China). Cotyledons were sampled from seedlings derived from dimorphic seeds (brown and black seeds) at different developmental stages (3, 18, and 33 days after emergence) or at different NaCl concentrations (0, 100, 300, and 500 mM). The quantity and quality of total RNA were determined with a Nanodrop ND-1000 UV spectrophotometer (Thermo Fisher Scientific, Waltham, MA, United States).

Cao J., Li X., Chen L., He M, & Lan H. (2022). The Developmental Delay of Seedlings With Cotyledons Only Confers Stress Tolerance to Suaeda aralocaspica (Chenopodiaceae) by Unique Performance on Morphology, Physiology, and Gene Expression. Frontiers in Plant Science, 13, 844430.

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