Casein, α-cornstarch, cellulose, vitamin mixture and mineral mixture were purchased from Oriental Yeast Co., Ltd. (Tokyo, Japan). MIN6 cells [28 (link)] were provided by Dr. J Miyazaki, Graduate School of Medicine, Osaka University (MTA13–148).
Mineral mixture
Manufactured by Oriental Yeast
Sourced in Japan
The Mineral mixture is a laboratory product that provides a balanced combination of essential minerals. It is designed to support various experimental and analytical applications in the laboratory setting. The specific composition and concentration of the minerals may vary depending on the formulation, catering to the diverse needs of researchers and scientists.
Automatically generated - may contain errors
Lab products found in correlation
Vitamin mixture, by Oriental Yeast (5 mentions)
Fetal bovine serum (fbs), by Merck Group (3 mentions)
Earle s buffered salt solution ebss, by Merck Group (3 mentions)
Mem vitamin solution, by Merck Group (3 mentions)
Cellulose powder, by Oriental Yeast (3 mentions)
Corn starch, by Oriental Yeast (3 mentions)
Soybean oil, by Nacalai Tesque (3 mentions)
Casein, by Oriental Yeast (2 mentions)
Dulbecco s modified eagle s medium dmem, by Nissui Pharmaceutical (2 mentions)
α cornstarch, by Oriental Yeast (1 mentions)
Williams e medium, by Merck Group (1 mentions)
Nutrient mixture f 12 ham ham s f12, by Merck Group (1 mentions)
Corn oil, by Fujifilm (1 mentions)
Recombinant human igf 1, by Astellas Pharma (1 mentions)
Anti phospho akt ser473 antibody, by Cell Signaling Technology (1 mentions)
Bovine insulin, by Merck Group (1 mentions)
Anti erk antibody, by Cell Signaling Technology (1 mentions)
Anti phospho erk antibody, by Cell Signaling Technology (1 mentions)
Anti akt antibody, by Cell Signaling Technology (1 mentions)
Hank s buffered salt solution, by Nissui Pharmaceutical (1 mentions)
5 protocols using mineral mixture
1
Cultivating MIN6 Cells for Diabetes Research
2
Detailed Animal Diet Composition
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For animal experimental diets, the vitamin mixture, mineral mixture, cellulose powder, and corn starch were purchased from Oriental Yeast Co. (Tokyo, Japan), while soybean oil was acquired from Nacalai Tesque (Tokyo, Japan). Solutions such as 10 × Earle’s buffered salt solution (EBSS) and 100 × MEM vitamin solution were purchased from Sigma-Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from Nissui Pharmaceutical Co. (Tokyo, Japan). Fetal bovine serum (FBS) was obtained from Sigma-Aldrich. Penicillin and streptomycin were obtained from Banyu Pharmaceutical Co., (Ibaraki, Japan). Other chemicals were of commercially available reagent grade.
3
Investigating IGF-I and Growth Hormone Effects
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For the experimental animal diets, the vitamin mixture, mineral mixture, cellulose powder, and corn starch were purchased from the Oriental Yeast Co. (Tokyo, Japan). At the same time, the soybean oil was acquired from the Nacalai Tesque (Tokyo, Japan), and the corn oil and sucrose were acquired from the Fujifilm Wako Pure Chemical Corporation (Osaka, Japan). Recombinant human IGF-I was obtained from Astellas Pharma Inc. (Tokyo, Japan). Nutrient Mixture F-12 Ham (Ham’s F12, #F6636, Sigma, St. Louis, MO, USA), William’s E medium (#W4125), 10× Earle’s buffered salt solution (EBSS), 100× MEM vitamin solution, and fetal bovine serum (FBS) were purchased from the Sigma Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM) was purchased from the Nissui Pharmaceutical Co. (Tokyo, Japan). Human recombinant growth hormone (Growject®) was obtained from the JCR Pharmaceuticals (Hyogo, Japan). Other reagent-grade chemicals used in this study were commercially available.
4
Optimized Cell Culture Reagents
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For animal experimental diets, vitamin mixture, mineral mixture, cellulose powder and corn starch were purchased from Oriental Yeast (Tokyo, Japan), and soybean oil was from Nacalai Tesque (Tokyo, Japan). 10× Earle’s buffered salt solution (EBSS) and 100× MEM vitamin solution were purchased from Sigma Aldrich (St. Louis, MO, USA). Dulbecco’s modified Eagle’s medium (DMEM), phosphate-buffered saline (PBS), and Hanks’ buffered salt solution were purchased from Nissui Pharmaceutical CO., (Tokyo, Japan). Fetal bovine serum (FBS) and bovine insulin were obtained from Sigma Aldrich (St. Louis, MO, USA). Penicillin and streptomycin were obtained from Banyu Pharmaceutical CO., (Ibaraki, Japan). Anti-β-actin antibody was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA USA). Anti-phospho-Akt (Ser-473) antibody, anti-Akt antibody, anti-phospho-Erk antibody and anti-Erk antibody were obtained from Cell Signaling Technology, Inc. (Danvers, MA, USA). Horseradish peroxidase (HRP)-conjugated secondary anti-rabbit and anti-mouse IgG antibody were obtained from GE Healthcare (Pittsburgh, PA, USA). Radiolabeled substances were purchased from PerkinElmer Life Science (Boston, MA, USA). Other chemicals were of the reagent grade available commercially.
5
Propolis-Enriched Diet for C57BL/6J Mice
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Mice and Diet Male C57BL/6J mice (Japan SLC, Shizuoka, Japan) were maintained under specific pathogen-free conditions with a 12-h light : dark cycle at 25±2°C and 55±10% relative humidity. The composition of food was 27.5% casein, 17.6% α-starch (Oriental Yeast Co., Ltd., Chiba, Japan), 8.3% sucrose (Mitsui Sugar Co., Ltd., Osaka, Japan), 36.0% lard (Oriental Yeast Co.), 2% cellulose, 5% mineral mixture, 1% vitamin mixture (Oriental Yeast Co., Ltd.), 0.3% DL-methio- nine (Wako Pure Chemical Industries, Ltd., Osaka, Japan), 0.2% choline bitartrate (Wako) and 2% dextrin (Wako). Brazilian propolis was supplied as a powder by Yamada Bee Farm (Okayama, Japan). Brazilian propolis is produced in Southeast Brazil, and Baccharis dracunculifolia is the main botanical source. It contains mainly prenylated derivatives of cinnamic acid including artepillin C as a major component (6.1%). In the experimental diet, propolis was added to the control diet at a dose of 2.0% (w/w) instead of dextrin. All experimental procedures were approved by the Animal Research Committee of the University of Tokushima.
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