Anhydrous sodium acetate, iron (III) chloridehexahydrat (FeCl3 · 6H2O), potassium dichromate, ethanol, 1,6-hexanediamine, ethylene glycol, nickel (II) chloride hexahydrat (NiCl2.6H2O), sodium hydroxide, hydrogen chloride, which were of analytical grade, were purchased from Merck, Germany and were used without further purification. potassium dichromate (99 %) and nickel (II) chloride hexahydrat (99 %) were used for preparation of Cr (VI) and Ni (II) solution. Additionally, doubly distilled deionized water was used throughout the work.
1 6 hexanediamine
Manufactured by Merck Group
Sourced in United States, Germany
1,6-hexanediamine is a chemical compound commonly used as a precursor in the synthesis of various polymers and pharmaceutical products. This aliphatic diamine has the chemical formula H2N(CH2)6NH2. It serves as a key building block in the production of nylon, polyurethanes, and other specialty chemicals. The core function of 1,6-hexanediamine is to provide a reactive amine group that can participate in polymerization and other chemical reactions.
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Lab products found in correlation
Ethanol, by Merck Group (2 mentions)
Chloroform, by Merck Group (2 mentions)
Anhydrous sodium acetate, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Merck Group (1 mentions)
Ethylene glycol, by Merck Group (1 mentions)
Potassium dichromate, by Merck Group (1 mentions)
Chitosan, by Merck Group (1 mentions)
Phosphate buffered saline (pbs), by Merck Group (1 mentions)
Polyvinylpyrrolidone, by Merck Group (1 mentions)
Glutaraldehyde, by Merck Group (1 mentions)
Isopropyl alcohol, by Merck Group (1 mentions)
Phosphate buffer saline (pbs), by Merck Group (1 mentions)
Sodium acetate, by Merck Group (1 mentions)
Absolute methanol, by Merck Group (1 mentions)
N n dimethylformamide (dmf), by Merck Group (1 mentions)
Sodium carbonate, by Merck Group (1 mentions)
Tetrabutyl titanate, by Merck Group (1 mentions)
Dimethyl carbonate, by Merck Group (1 mentions)
Poly ε caprolactone, by Merck Group (1 mentions)
Milli q integral system, by Merck Group (1 mentions)
12 protocols using 1 6 hexanediamine
1
Synthesis of Cr(VI) and Ni(II) Solutions
2
Chitosan-Collagen Composite Scaffold
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Chitosan (high molecular weight; degree of deacetylation = 85%) and phosphate-buffered saline (PBS) were obtained from Sigma-Aldrich. Bovine collagen type I (AteloCollagen) with molecular weight of 300 KDa was purchased from Wuxi BIOT Biology Technology Co. LTD (china). Glacial acetic acid and 2-propanol (99.5%) were purchased from Dr. Mojallali Co. (Iran). PCL (Mn= 80000), Polyvinylpyrrolidone (PVP) (Mw= 360000), glutaradialdehyde (GA) 25%, and 1,6- Hexanediamine (HDA) were provided by Merck. Ethanol (99.8%) was purchased from Simin Taak. Co (Iran).
3
Biomimetic Bone Graft Fabrication
4
Polycarbonate Diol Synthesis and Characterization
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1,6-Hexanediamine (1,6-HDA,
99.5%), dimethyl carbonate (DMC, 99%), sodium acetate, absolute methanol,
chloroform, tetra butyl titanate (TBT), N,N-dimethylformamide (DMF), and sodium carbonate were obtained
from Sigma Aldrich. Tetrahydrofuran (THF, 99.9%) was obtained from
Carl Roth. Eternacoll UH50 (PCDL500, OH#: 224, 500 g/mol),
Eternacoll UH100 (PCDL1000, OH#: 110, 1000 g/mol), and
Eternacoll UH200 (PCDL2000, OH#: 56, 2000 g/mol) as aliphatic
polycarbonate diols (PCDLs) were kindly supplied from UBE Corporation
Europe. 1,1,1,3,3,3-Hexafluoroisopropanol (HFIP) was obtained from
Iris Biotech GmbH.
99.5%), dimethyl carbonate (DMC, 99%), sodium acetate, absolute methanol,
chloroform, tetra butyl titanate (TBT), N,N-dimethylformamide (DMF), and sodium carbonate were obtained
from Sigma Aldrich. Tetrahydrofuran (THF, 99.9%) was obtained from
Carl Roth. Eternacoll UH50 (PCDL500, OH#: 224, 500 g/mol),
Eternacoll UH100 (PCDL1000, OH#: 110, 1000 g/mol), and
Eternacoll UH200 (PCDL2000, OH#: 56, 2000 g/mol) as aliphatic
polycarbonate diols (PCDLs) were kindly supplied from UBE Corporation
Europe. 1,1,1,3,3,3-Hexafluoroisopropanol (HFIP) was obtained from
Iris Biotech GmbH.
5
Poly(ε-caprolactone) and Polyelectrolyte Composites
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Poly(ε-caprolactone) (PCL; Mw = 82 kDa), poly(sodium4-styrenesulfonate) (PSS; Mw = 70 kDa), 1,6-hexanediamine (ED), chloroform (≥99.9%), formic acid (≥95%), and sodium acetate buffer solution were purchased from Sigma-Aldrich, UK. Poly(allylamine hydrochloride) (PAH) was supplied from Alfa Aesar, UK, while medical-grade MH (400 mg/kg of methylglyoxal) was purchased from ManukaGuard®, USA. Ultrapure water was obtained by a Milli-Q® Integral system (Merck, Italy). All materials and chemicals were used without further purification.
6
Polyamine Analysis in Goose Granulosa Cells
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The polyamine content in the goose GCs was determined by high-performance liquid chromatography according to the methods of Kang et al. (2017b) (link). The general steps were as follows. First, polyamine standard curves were prepared. Goose GCs were ultrasonically lysed, and 1 mL of 5% perchloric acid and 10 μL of 1,6-hexanediamine (Sigma, Shanghai, China) standard working solution were added. Then, the samples were sonicated for 10 min and centrifuged, and the supernatant was collected. Then, the samples were extracted again with 1 mL of 5% perchloric acid, the supernatant was removed, an equal volume of 2.5 mol/L NaOH and 7 μL of benzoyl chloride was added, and the samples were derivatized at 40°C for 1 h. The samples were adjusted to a neutral pH with 6 mol/L HCl, and a HyperSep C18 extraction column (Thermo Fisher Scientific) was used to extract and separate the derivative products. The extraction column was washed with 15 mL of ultrapure water and 15 mL of 15% (v/v) chromatographic grade aqueous methanol solution to purify the derivative products. Methanol was added to the extraction column for elution. The methanol: ultrapure water ratio was 62: 38 (v/v), and the column temperature of the Hypurity C18 chromatographic separation column was 40°C. The results were compared with the standard curve of polyamine.
7
Trimethylene Carbonate Polymerization Initiators
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Trimethylene carbonate (TMC) comes from Foryou Medical (Huizhou, China). The initiators (1,6-hexanediol, (99%), propylamine, benzylamine, 1,6-hexanediamine, p-xylenediamine, tris(2-aminoethyl)amine, bis(3-aminopropyl)amine, glycine methyl ester, L-phenylalanine methyl ester, the catalysts methanesulfonic acid (MSA), 1,5,7-triazabicyclo[4.4.0]dec-5-ene (TBD), tin(II) 2-ethylhexanoate (Sn(Oct)2), were purchased from Sigma Aldrich (Saint-Quentin Fallavier, France) and used as received. Anhydrous dichloromethane was retrieved from solvent purificator Inert PureSolv™ (Castelnau-le-Lez, France) and used as the polymerization solvent.
8
RGD Peptide-Based Cell Viability Assay
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RGD peptides (Purity 98.68%) were purchased from GL Biochem Ltd. (Shanghai, China). Ninhydrin assay and 1,6-hexanediamine were purchased from Sigma-Aldrich (United States). Acetic acid and 2-propanol were purchased from JT Baker (United States). Glutaraldehyde was purchased from R&M (Malaysia). H9c2 myoblast cell lines [H9c2(2-1)] (ATCC® CRL-1446TM), Dulbecco’s Modified Eagle’s Medium (DMEM) (ATCC® 30-2002TM), fetal bovine serum (FBS) (ATCC® 30-2021TM) and trypsin/EDTA (ATCC® 30-2101TM) were purchased from ATCC. CellTiter 96® AQueous One Solution Reagent was purchased from Promega (United States). DMSO was purchased from Invitrogen (United States). Phosphate buffer solution (PBS) and trypan blue stain were purchased from Gibco (United States).
9
Polyamine Quantification Protocol
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Putrescine standard, spermidine standard, spermine standard and 1,6-hexanediamine were purchased from Sigma (St. Louis, MO, USA); Protein Maker, ECL chromogenic solution and PVDF membrane were purchased from BIO-RAD Biological Company; PBS, PMSF, RIPA, primary antibody diluent and secondary antibody diluent were purchased from Beyotime Co., Ltd. (Haimen, China); chromatographic methanol was purchased from Bio-Rad Yu (Fisher Scientific, Waltham, MA, USA); SYBR Green Supermix was purchased from TaKaRa (Kusatsu, Japan); PMSG was purchased from Ningbo Sunshine Biological (Wuxi, China); perchloric acid, sodium hydroxide, concentrated hydrochloric acid, benzoyl chloride, anhydrous ethanol and all other reagents were analytically pure and purchased from Ruijinte (Chengdu, China).
10
Nitrogen-doped Carbon Dots Synthesis
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Carbon dots (CDs) were prepared by a hydrothermal approach from lactose, as previously addressed in detail [36] (link). Briefly, lactose was treated in a Teflon lined steel reactor with HCl (1 M) at 100 °C for 3 h in an oven. Afterwards, the mixture was dialyzed against deionized water for 24 h. N-doped CDs (N-CDs) were prepared by mixing CDs with 1,6-hexanediamine (1 g, 98%, Sigma-Aldrich, Germany) in a Teflon lined steel reactor, and heated in an oven for 3 h at 100 °C (Scheme 1). It was assumed that a high solubility of 1,6hexanediamine in water and its nitrogen chemistry will promote the incorporation of N-containing groups to CDs' .
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