Mda mb 134 6 mm134

MCF-7, MCF-10A, MDA-MB-134-VI (MM134), MDA-MB-330 (MM330), and HEK293T cells were obtained from ATCC. Sum44PE cells were purchased from Asterand. BCK4 cells were a generous gift from Britta Jacobsen, PhD (University of Colorado Anschutz Medical Campus) and recently described^{24 (link), 25 (link)}. Media formulations for cell culture are outlined in the supplemental text. Cells were periodically confirmed to be mycoplasma negative, authenticated at the University of Arizona Genetics Core by Short Tandem Repeat (STR) profiling and kept in continuous culture for less than 6 months. To assay TP53 status in cell lines, PCR was performed on cDNA (primers listed in Table S4) covering the full length TP53 transcript using Gotaq DNA Polymerase (Promega# M3001) as per manufacturer’s protocol. Sequenced PCR products were blasted against the human reference TP53 sequences (NM_000546).

MCF7 (RRID: CVCL_0031), T47D (RRID: CVCL_0553), MDA-MB-134-VI (MM134, RRID: CVCL_0617), MCF10A (ATCC Cat# CRL-10317, RRID:CVCL_0598) and HEK293T (RRID: CVCL_0063) were all purchased from American Type Culture Collection (ATCC). SUM44-PE cells (SUM44, CVCL_3424) were purchased from Asterand and BCK4 cells were kindly provided by Britta Jacobsen, University of Colorado Anschutz, CO. MCF7 with ESR1-Y537S were generated previously(24 (link)). Cell identity was authenticated by by Short Tandem Repeat DNA profiling (University of Arizona), and cells were routinely tested to be mycoplasma free. Cells were maintained in continuous culture for less than 6 months with media indicated in supplementary materials.