The proliferative capacity of the transfected cell was assessed using the bromodeoxyuridine (Brdu) incorporation assay kits (Millipore, Billerica, MA, USA). Briefly, cells (1×104 (link)) were seeded in each well of a 96-well plate after 48 hours of transfection. 10 µL of Brdu was subsequently added to each well. After 5 hours incubation, cells were fixed by FixDenat for 30 minutes. Cells were then incubated with anti-Brdu antibody in incubation buffer from Roche for 1 hour at 37°C. Cell nuclei were stained with 1 µM SYTOX Green (S7020, Life Technologies) at room temperature for 15 minutes. In each treatment, cells were washed with PBS at least three times.
Brdu incorporation assay kits
Manufactured by Merck Group
Sourced in United States
The BrdU (Bromodeoxyuridine) Incorporation Assay Kits are laboratory tools used to detect and quantify cell proliferation. They measure the incorporation of the synthetic thymidine analog BrdU into the DNA of actively dividing cells, providing an indirect measure of DNA synthesis and cell division.
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Lab products found in correlation
2 protocols using brdu incorporation assay kits
1
Cell Proliferation Assay with Brdu
2
Neural Stem Cell Proliferation Assay
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Neural stem cell proliferation was measured using the bromodeoxyuridine (BrdU) incorporation assay kits (Millipore, Billerica, MA, USA). Briefly, 104 cells were seeded in each well of a 96‐well plate with morin with indicated concentrations followed by 48 hrs of incubation. Bromodexyuridine was added at hour 46 of incubation. The following steps of the assay were carried out according to the manufacturer's instructions.
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