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Human timp2 cdna

Manufactured by Thermo Fisher Scientific
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The Human TIMP2-cDNA is a complementary DNA (cDNA) that encodes the tissue inhibitor of metalloproteinase 2 (TIMP2) protein. TIMP2 is a member of the TIMP family of proteins, which are known to inhibit the activity of matrix metalloproteinases (MMPs). The cDNA is used as a tool in research applications to study the expression, regulation, and function of the TIMP2 gene and protein.

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Lab products found in correlation

Mrfp rab5, by Addgene (2 mentions) Mda mb 231ln, by PerkinElmer (2 mentions) Hs578t, by American Type Culture Collection (2 mentions) Hek293t, by American Type Culture Collection (2 mentions)

2 protocols using human timp2 cdna

1

Molecular Tools for Cell Line Analysis

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines HEK293T (ATCC), Hs578T (ATCC), and MDA-MB-231LN (Caliper LifeScience) were grown based on the manufacturer's recommendations. shRNAs and siRNAs against NEDD9, non-targeting control (ThermoFisher Scientific), and NEDD9-cDNA were prepared as previously described (7 (link)). mRFP-Rab5, and -Rab7 were purchased from Addgene (plasmid #14437, #14436) (60 (link)). Plasmids mCherry-MMP14 and PAmCherry-MMP14 were a gift from Dr.Chavrier (Institute Curie, France) and Dr.Machesky (The Beatson Institute for Cancer Research, Scotland) (24 (link)). Human TIMP2-cDNA (ThermoFisher Scientific) was sub-cloned into pcDNA3.1-3x-Flag plasmid (gift from Dr.Frisch, West Virginia University, USA).
Loskutov Y.V., Kozyulina P.Y., Kozyreva V.K., Ice R.J., Jones B.C., Roston T.J., Smolkin M.B., Ivanov A.V., Wysolmerski R.B, & Pugacheva E.N. (2014). NEDD9/Arf6-Dependent Endocytic Trafficking of Matrix Metalloproteinase 14: A Novel Mechanism for Blocking Mesenchymal Cell Invasion and Metastasis of Breast Cancer. Oncogene, 34(28), 3662-3675.
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2

Molecular Tools for Cell Line Analysis

Cited 3 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lines HEK293T (ATCC), Hs578T (ATCC), and MDA-MB-231LN (Caliper LifeScience) were grown based on the manufacturer's recommendations. shRNAs and siRNAs against NEDD9, non-targeting control (ThermoFisher Scientific), and NEDD9-cDNA were prepared as previously described (7 (link)). mRFP-Rab5, and -Rab7 were purchased from Addgene (plasmid #14437, #14436) (60 (link)). Plasmids mCherry-MMP14 and PAmCherry-MMP14 were a gift from Dr.Chavrier (Institute Curie, France) and Dr.Machesky (The Beatson Institute for Cancer Research, Scotland) (24 (link)). Human TIMP2-cDNA (ThermoFisher Scientific) was sub-cloned into pcDNA3.1-3x-Flag plasmid (gift from Dr.Frisch, West Virginia University, USA).
Loskutov Y.V., Kozyulina P.Y., Kozyreva V.K., Ice R.J., Jones B.C., Roston T.J., Smolkin M.B., Ivanov A.V., Wysolmerski R.B, & Pugacheva E.N. (2014). NEDD9/Arf6-Dependent Endocytic Trafficking of Matrix Metalloproteinase 14: A Novel Mechanism for Blocking Mesenchymal Cell Invasion and Metastasis of Breast Cancer. Oncogene, 34(28), 3662-3675.
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