Log In Sign up
The largest database of trusted experimental protocols

Capto core 700 instrument

Manufactured by GE Healthcare
Visit Supplier

The Capto Core 700 is a laboratory instrument designed for versatile chromatographic separations. It features a modular design and accommodates a wide range of chromatography resins, enabling efficient purification of various biomolecules. The instrument provides consistent, reliable performance to support research and development activities in the life sciences and biopharmaceutical industries.

Automatically generated - may contain errors

Lab products found in correlation

Pyrophosphatase enzymes, by Aldevron (2 mentions) Bspqi restriction enzyme, by New England Biolabs (2 mentions) T7 polymerase, by Aldevron (2 mentions) Rnase inhibitor, by Aldevron (2 mentions) S adenosyl methionine, by Aldevron (2 mentions)

Close spelling variants of this product

Capto Core 700

2 protocols using capto core 700 instrument

1

Scalable RNA Production for Vaccine Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNAs encoding the 20 lead mAb candidates were amplified from the mammalian expression pTwist vector using universal primer sets and cloned into plasmids encoding Venezuelan equine encephalitis virus replicon (strain TC-83) under the control of a T7 promoter (pT7-VEE-Rep)32 (link). To generate linear templates for RNA transcription for RNA in vitro transcription and capping at midi scale (500 μg), plasmid DNA was cleaved using NotI or BspQI restriction enzymes (New England Biolabs), respectively, and purified using phenol-chloroform extraction and sodium acetate precipitation. RNA was transcribed using T7 Polymerase, RNAse inhibitor, Pyrophosphatase enzymes (Aldevron) and reaction buffer. RNA transcripts were capped with vaccinia virus capping enzyme using GTP and S-adenosyl-methionine (Aldevron) as substrates to create cap-0 structures. RNA was purified using lithium chloride precipitation. Large-scale capability demonstration RNA production was performed using a 200 mg scaled-up process and purified via column on a Capto Core 700 instrument (GE Healthcare Life Sciences) and tangential flow filtration.
Gilchuk P., Bombardi R.G., Erasmus J.H., Tan Q., Nargi R., Soto C., Abbink P., Suscovich T.J., Durnell L.A., Khandhar A., Archer J., Liang J., Fouch M.E., Davidson E., Doranz B.J., Jones T., Larson E., Ertel S., Granger B., Fuerte-Stone J., Roy V., Broge T., Linnekin T.C., Linde C.H., Gorman M.J., Nkolola J., Alter G., Reed S.G., Barouch D.H., Diamond M.S., Crowe JE J.r., Van Hoeven N., Thackray L, & Carnahan R. (2020). Integrated pipeline for the accelerated discovery of antiviral antibody therapeutics. Nature biomedical engineering, 4(11), 1030-1043.
+ Open protocol
+ Expand
2

Scalable RNA Production for Vaccine Research

Check if the same lab product or an alternative is used in the 5 most similar protocols
cDNAs encoding the 20 lead mAb candidates were amplified from the mammalian expression pTwist vector using universal primer sets and cloned into plasmids encoding Venezuelan equine encephalitis virus replicon (strain TC-83) under the control of a T7 promoter (pT7-VEE-Rep)32 (link). To generate linear templates for RNA transcription for RNA in vitro transcription and capping at midi scale (500 μg), plasmid DNA was cleaved using NotI or BspQI restriction enzymes (New England Biolabs), respectively, and purified using phenol-chloroform extraction and sodium acetate precipitation. RNA was transcribed using T7 Polymerase, RNAse inhibitor, Pyrophosphatase enzymes (Aldevron) and reaction buffer. RNA transcripts were capped with vaccinia virus capping enzyme using GTP and S-adenosyl-methionine (Aldevron) as substrates to create cap-0 structures. RNA was purified using lithium chloride precipitation. Large-scale capability demonstration RNA production was performed using a 200 mg scaled-up process and purified via column on a Capto Core 700 instrument (GE Healthcare Life Sciences) and tangential flow filtration.
Gilchuk P., Bombardi R.G., Erasmus J.H., Tan Q., Nargi R., Soto C., Abbink P., Suscovich T.J., Durnell L.A., Khandhar A., Archer J., Liang J., Fouch M.E., Davidson E., Doranz B.J., Jones T., Larson E., Ertel S., Granger B., Fuerte-Stone J., Roy V., Broge T., Linnekin T.C., Linde C.H., Gorman M.J., Nkolola J., Alter G., Reed S.G., Barouch D.H., Diamond M.S., Crowe JE J.r., Van Hoeven N., Thackray L, & Carnahan R. (2020). Integrated pipeline for the accelerated discovery of antiviral antibody therapeutics. Nature biomedical engineering, 4(11), 1030-1043.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!