Dooku1

Yoda1, ruthenium red, and Dooku1 were purchased from Tocris (Avonmouth, Bristol, UK). Capsaicin was purchased from Sigma (St. Louis, MO, USA) and the stock solutions were made with 99.5% ethanol. All stock solutions were stored at −20 °C.

Fluorescein isothiocyanate-conjugated lactadherin (LA-FITC) and phalloidin (FITC-phalloidin) came from Haematologic Technologies Inc. (Essex Junction, VT, USA), supplied by Cambridge Bioscience (Cambridge, UK). Anti-glycophorin A-PE from Becton Dickinson Biosciences (CA, USA) came from Enzyme Research Laboratories (Swansea, UK). Bromo-A23187, 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) and Fluo-4-AM came from Calbiochem (Merck, Darmstadt, Germany). Yoda1, Dooku1 and GsMTx4 were supplied by Tocris Bioscience (Abingdon, UK), Glixx Laboratories Inc (Hopkinton, MA, USA) and AbCam (Cambridge, UK); and AbCam also supplied the PKC inhibitors chelerytherine chloride and calphostin C. All other chemicals were supplied by Sigma-Aldrich Co. (Poole, Dorset, UK).