O 2 mercaptoethyl o methyl hexa ethylene glycol

Manufactured by Merck Group

O-(2-mercaptoethyl)-O′-methyl-hexa(ethylene glycol) is a polyethylene glycol derivative used in various biochemical and analytical applications. It serves as a linker or spacer molecule, allowing the attachment of other compounds or entities to surfaces or substrates. The compound possesses a reactive thiol (sulfhydryl) group and a methyl-terminated polyethylene glycol chain, making it useful for conjugation and immobilization procedures.

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Lab products found in correlation

P toluenesulfonic acid, by Merck Group (1 mentions) Hexa ethylene glycol dithiol, by Merck Group (1 mentions) Poly ethylene glycol methyl ether thiol, by Merck Group (1 mentions) Milli q system, by Merck Group (1 mentions) Ethyl vinyl ether, by Merck Group (1 mentions) Bstui, by New England Biolabs (1 mentions) Gold enhancement kit, by Nanoprobes (1 mentions) 11 mercaptoundecyl tetra ethylene glycol, by Merck Group (1 mentions) Oligonucleotides, by Biomers (1 mentions)

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Ethylene glycol (580 citations)

2 protocols using o 2 mercaptoethyl o methyl hexa ethylene glycol

Purification and Characterization of Glycol-Thiols

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All solvents (methanol, dichloromethane, and tetrahydrofuran) as well as ethyl vinyl ether (99%, Aldrich) were purified by distillation. Deionized water was obtained from a Millipore MilliQ system and additionally filtered through a 220 nm PTFE filter. Allyl glycidyl ether (99% Fluka) was distilled under reduced pressure. KOH (Merck), 1,2-propandiol (Aldrich), benzene (Merck), p-toluenesulfonic acid (Merck), and 1,6-diphenyl-1,3,5-hexatriene (Aldrich) were used without purification. Hexa(ethylene glycol)dithiol (Sigma-Aldrich, > 97%, M_n = 314.46 g mol⁻¹) (HEGDT), O-(2-mercaptoethyl)-O′-methyl-hexa(ethylene glycol) (Sigma-Aldrich, ≥ 95%, M_n = 356.48 g mol⁻¹) (HEGMT) and poly(ethylene glycol)methyl ether thiol (Sigma-Aldrich, M_n = 2000 g mol⁻¹) (PEGMET) were used as received.

Toncheva-Moncheva N., Dangalov M., Vassilev N.G, & Novakov C.P. (2020). Thiol–ene coupling reaction achievement and monitoring by “in situ” UV irradiation NMR spectroscopy. RSC Advances, 10(42), 25214-25222.

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Nanoparticle-based Molecular Assay

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All oligonucleotides are purchased from Biomers or IDT. Gold nanoparticles are purchased from BB International, silver enhancement kit from Invitrogen and gold enhancement kit from Nanoprobes. All enzymes are purchased from Fermentas except for BstUI which is purchased from New England Biolabs and Tth ligase that is purchased from Genecraft. O-(2-Mercaptoethyl)-O′-methylhexa(ethylene glycol) and (11-mercaptoundecyl)tetra(ethylene glycol) are purchased from Sigma-Aldrich.

Russell C., Welch K., Jarvius J., Cai Y., Brucas R., Nikolajeff F., Svedlindh P, & Nilsson M. (2014). Gold Nanowire Based Electrical DNA Detection Using Rolling Circle Amplification. ACS Nano, 8(2), 1147-1153.

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