Plasma lipids were measured on Days 0, 15 and 30. The plasma triglycerides (TG), total cholesterol (TC), Low-density lipoprotein (LDL), High-density lipoprotein (HDL), and Very-low density lipoprotein (VLDL) were measured using the Vitros DT60 II Chemistry System (Johnson & Johnson, Minnesota, MN). The liver samples were homogenized using a Stir-Pak®, (Barrington, Il). T-Chol and TG were extracted in a chloroform-methanol mixture (2:1) and measured with the same Vitros DT60 II Chemistry System.
Vitros dt60 2 chemistry system
Manufactured by Johnson & Johnson
Sourced in United States
The Vitros DT60 II Chemistry System is a diagnostic lab equipment product manufactured by Johnson & Johnson. It is designed to perform automated clinical chemistry analyses. The system utilizes a dry slide technology to analyze a variety of analytes from patient samples.
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Lab products found in correlation
4 protocols using vitros dt60 2 chemistry system
1
Plasma Lipid Profiling in Research
2
Plasma Lipid Analysis Protocol
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Plasma lipids were measured on days 0 and 35. Plasma low-density lipoprotein (LDL), triglycerides (TG), total cholesterol (CHO), high-density lipoprotein (HDL), and very LDL were measured using the Vitros DT60 II Chemistry System (Johnson & Johnson, Minneapolis, MN). The liver samples were homogenized using a Stir-Pak® (Barrington, IL). Total CHO and TG were extracted in a chloroform-methanol mixture (2:1) and measured with the same Vitros DT60 II Chemistry System.
3
Serum Lipid Profile Measurement
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Venous blood was collected from brachial veins after fasting for 10-12 h. Collected blood was immediately centrifuged, and the serum was then stored at -80 °C until use. The serum samples were examined for total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), and TGs using a Vitros DT60II Chemistry System (Johnson & Johnson, NY, USA). Blood HDL-C levels were analyzed following the precipitation of cholesterol with apolipoprotein B using magnesium chloride and dextran sulfate. Low-density lipoprotein cholesterol (LDL-C) levels were calculated using the following formula: LDL-C = TC -(HDL-C+(TG/5)) (31) .
4
Plasma Lipid Profiling Assay
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Plasma lipids were measured on Days 0, 15, and 30. Plasma triglycerides (TG), total cholesterol (TC) levels, low-density lipoproteins (LDL), high-density lipoproteins (HDL), and very-low density lipoproteins (VLDL) were measured using the Vitros DT60 II Chemistry System (Johnson & Johnson, Minnesota, MN). The liver samples were homogenized using a Stir-Pak®, (Barrington, Il). TC and TG were extracted in a chloroform-methanol mixture (2:1) and measured with the same Vitros DT60 II Chemistry System.
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