Chromatographic analysis was performed using an Agilent 1260 liquid chromatography system (Agilent Technology, Inc., Santa Clara, CA, USA), which was equipped with a G1311C quat pump, a G1329B autosampler, a G1316A column compartment, and a G1315D diode array detection (DAD) system. The obtained data were analyzed on an Agilent open LAB CDS ChemStation C.01.04. The analysis of fingerprint was carried out at 25 °C on an Agilent ZORBAX 300SB-C18 column (4.6 × 250 mm, 5 μm). The elution gradient of eluents A (acetonitrile with 0.1% TFA) and B (water with 0.1% TFA) was used for the separation of the target analytes. The gradient program was as follows: 0–40 min, 5–77% A; 40–58 min, 77–95% A. Each run was followed by an equilibration time of 5 min. The UV absorbance was monitored at 215 nm, and the solvent flow rate was kept at 1.0 mL/min. All injection volumes of both the samples were 5 μL.
G1329b autosampler
Manufactured by Agilent Technologies
Sourced in United States
The G1329B autosampler is a lab equipment product from Agilent Technologies. It is designed to automatically inject samples into a chromatographic system for analysis. The core function of the G1329B autosampler is to transport and inject liquid samples into the system precisely and consistently.
Automatically generated - may contain errors
Lab products found in correlation
G1311c quaternary pump, by Agilent Technologies (3 mentions)
G1316a column compartment, by Agilent Technologies (2 mentions)
G1315d diode array detector, by Agilent Technologies (2 mentions)
Agilent 1260 hplc system, by Agilent Technologies (2 mentions)
Optilab t rex detector, by Wyatt Technology (2 mentions)
G1310b iso pump, by Agilent Technologies (2 mentions)
G1314f variable wavelength detector, by Agilent Technologies (2 mentions)
Zorbax 300sb c18 column, by Agilent Technologies (1 mentions)
Agilent 1260, by Agilent Technologies (1 mentions)
Agilent 1200 infinity, by Agilent Technologies (1 mentions)
Auw220d, by Shimadzu (1 mentions)
Barnstead smart2pure water purification system, by Thermo Fisher Scientific (1 mentions)
Agilent 1260 infinity thermostatted column compartment, by Agilent Technologies (1 mentions)
Agilent 1260 infinity quaternary pump, by Agilent Technologies (1 mentions)
Pack ods column, by YMC (1 mentions)
Agilent chemstation software, by Agilent Technologies (1 mentions)
G1315d dad, by Agilent Technologies (1 mentions)
G1311b quaternary pump, by Agilent Technologies (1 mentions)
Lc 20ad pump, by Shimadzu (1 mentions)
Lc 20a hplc system, by Shimadzu (1 mentions)
12 protocols using g1329b autosampler
1
Fingerprint Analysis of Compounds by HPLC-DAD
2
Analytical Method Development for Chromatographic Analysis
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The liquid chromatographic system consisted of an Agilent 1200 Infinity series high-performance liquid chromatography (LC) system (Agilent Technologies, Deutschland, Germany) supported by OpenLAB software version A.01.03 and equipped with a G1314B Agilent 1260 Infinity Variable Wavelength UV Detector. The temperature was controlled using a G1316A column oven with a G1330B Agilent 1260 Infinity Thermostatted Column Compartment. The LC system also had a G1311C Agilent 1260 Infinity Quaternary Pump and a G1329B Autosampler. A dissolution tester (DS 800, Lab India, Pvt. Ltd., Mumbai, India) was used in the study. A double-beam T90+ UV/Vis spectrophotometer supported by the UVWIN software version 5.2.0 (PG Instruments Ltd., Leicestershire, United Kingdom) and quartz cuvettes with a path length of 1 cm were used.
A Barnstead Smart2Pure™ water purification system (Thermo Fisher Scientific, Massachusetts, United States) was used to obtain ultrapure water. A Shimadzu AUW220D semi-micro-analytical electronic weighing balance (Shimadzu Corporation, Kyoto, Japan) with a sensitivity of ±0.1 mg was used for weighing. All the mobile phase preparations were degassed using a MRC DC-200H Ultrasonic Cleaner (MRC Lab Ltd., Holon, Israel).
A Barnstead Smart2Pure™ water purification system (Thermo Fisher Scientific, Massachusetts, United States) was used to obtain ultrapure water. A Shimadzu AUW220D semi-micro-analytical electronic weighing balance (Shimadzu Corporation, Kyoto, Japan) with a sensitivity of ±0.1 mg was used for weighing. All the mobile phase preparations were degassed using a MRC DC-200H Ultrasonic Cleaner (MRC Lab Ltd., Holon, Israel).
3
HPLC Analysis of Herbal Extract
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The high-performance liquid chromatography (HPLC) system consisted of an Agilent infinity series 1260 liquid chromatography system with a G1311B quaternary pump, G1329B autosampler, G1316A column oven and G1315D DAD detector, connected to Agilent ChemStation software (Agilent, Waldbronn, Germany). The separation was conducted with a YMC-PACK ODS column (4.6 mm × 250 mm, 5 μm). The gradient profile was as follows: 0–5 min, initial mobile phase of 0.1% formic acid prepared in acetonitrile and 0.1% formic acid prepared in water (10:90); 5–45 min, linear gradient of 70:30; and 45–50 min, isocratic 70:30. The flow rate was 1.0 ml/min, and detection was at 254 nm. The sample concentration was 10 mg/min in MeOH, and the injection volume was 10 μl. The HPLC chromatogram of SCE is shown in Fig. 1B .
4
Chiral HPLC Analysis of Benoxacor Enantiomers
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In the normal-phase chiral HPLC analysis, benoxacor enantiomers were separated with a Shimadzu LC-20A HPLC system (Tokyo, Japan), which included two LC-20AD pumps, a DGU-20A degasser, a CTO-20A column oven, a SIL-20AD sample injector, and an SPD-20A photodiode array detector. Signal collection and data analysis were processed by Shimadzu Labsolution (Tokyo, Japan). In the reversed-phase analysis, an Agilent 1260 series HPLC was used to separate benoxacor enantiomers, which included a G1311B pump, a G1315D diode array detector, a G1329B autosampler, a G1316A column compartment, and a G1322A degasser (Santa Clara, CA, USA). Signals were collected and analyzed using an Agilent Chemstation.
5
Quantification of BZH in Spray-Dried Powders
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BZH in the spray-dried powders was quantified by high pressure liquid chromatography (HPLC). Approximately 5 mg of spray-dried powders was dissolved into 1 mL of deionized water, vortexed for 10 min and further sonicated for 30 min to ensure the drug was fully dissolved. Each sample was passed through a cellulose ester syringe filter with pore size of 0.45 µm before HPLC analysis to determine the drug content.
The HPLC instrument of Agilent 1260 infinity consisted of G1312B pump, G1329B autosampler, and G1314F UV detector. For the analysis of BZH, the drug samples (50 µL) were injected into the HPLC column (5 µm C18, 250 × 4.6 mm, 80 Å, ZORBAX Eclipse XDB, Agilent, Santa Clara, CA, USA) and eluted using a mobile phase (flow rate = 0.5 mL/min) of acetate buffer solution (pH3 adjusted by acetic acid): acetonitrile (60:40 v/v), and detected at 308 nm [37 (link)]. The retention time for BZH under these conditions was 6.5 min.
The HPLC instrument of Agilent 1260 infinity consisted of G1312B pump, G1329B autosampler, and G1314F UV detector. For the analysis of BZH, the drug samples (50 µL) were injected into the HPLC column (5 µm C18, 250 × 4.6 mm, 80 Å, ZORBAX Eclipse XDB, Agilent, Santa Clara, CA, USA) and eluted using a mobile phase (flow rate = 0.5 mL/min) of acetate buffer solution (pH3 adjusted by acetic acid): acetonitrile (60:40 v/v), and detected at 308 nm [37 (link)]. The retention time for BZH under these conditions was 6.5 min.
6
HPLC Method for Quantifying Furosemide
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The concentrations of furosemide were quantified using a modified HPLC method (Jankowski, Skorek-Jankowska & Lamparczyk, 1997 (link); Ma et al., 2014 (link)). Chromatographic analysis was performed on an Agilent 1260 HPLC system (Agilent Technologies, Santa Clara, CA, USA) consisting of a G1311C Quaternary pump, G1329B autosampler (0.1–100 μL), G1316A column oven (273–333 K), and G1315D Diode Array Detector (190–950 nm). The analytical column was a ZORBAX SB-C18 columm (4.6 × 150 mm, 5 µm; Agilent, Santa Clara, CA, USA) with a precolumn. The mobile phase was a mixture of acetonitrile and distilled water (69:31, v/v) with 0.3 M sodium acetate buffer (pH 5.0). The column temperature is 30 °C with a flow rate of 1 ml/min. The injection volume was 10 μl, and the detection wavelength was set at 280 nm.
7
Quantitative HPLC Analysis of EGCG-β-CD Complex
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10 mg of the EGCG–β-CD complex was dissolved in 1 mL ultrapure water to generate a 10 mg mL−1 stock solution. The stock solution of the EGCG–β-CD complex was diluted 10-fold, filtered through a 0.45 μm membrane and subjected to HPLC to determine the content of EGCG in the sample. A 10 μL sample was analyzed using an Agilent 1260 series HPLC system equipped with a G1311B Quat pump (1.0 mL min−1), a G1329B autosampler, a G1316A TCC column oven (40 °C), and a G1314F ultraviolet detector (280 nm), controlled using 1260LC Agilent ChemStation software (Agilent Technologies). The separation was completed using a C18 ODS column (ZORBAX SB-C18 4.6 mm × 250 mm, 5 microns, Agilent, USA). The mobile phases used in HPLC analysis were solvents A (100% acetonitrile) and B (0.03% trifluoroacetic acid), which were filtered through a 0.45 μm filter. The mobile phase A was increased from 10 to 60% over 36 min.30 (link)
8
Automated CPC-HPLC Metabolite Profiling
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CPC was performed on an Armen fully integrated SCPC-100+1000 CPC spot instrument (Armen Instruments, St-Ave, France). This instrument is a fully automated system consisting of a CPC column compartment (1000 mL rotor made of 21 stacked disks with a total of 1512 twin cells), a pump, an injector, a UV/vis detector, a fraction collector, a digital screen flat PC, and Armen Glider CPC software. The HPLC analysis was performed by an Agilent 1260 HPLC system (Agilent Technologies, Palo Alto, CA, USA): G1312C binary pump, a G1329B autosampler, a G1315D DAD detector, a G1316A column oven and ChemStation software. HPLC-grade solvents were purchased from Fisher Scientific (Pittsburgh, PA, USA). All other organic solvents used for extraction and CPC operation were analytical grade and obtained from Daejung (Gyonggi-do, Korea).
9
Analytical Techniques for Natural Product Characterization
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Mass spectra were obtained using a Bruker micro-TOFQ mass spectrometer (Bruker Daltonics, Bremen, Germany). Nuclear magnetic resonance spectra were acquired with a Bruker AV-500 FT-NMR spectrometer operating at 500.1 MHz for 1H and at 125.8 MHz for 13C at 25 °C; chemical shifts are expressed in δ referring to the residual solvent signals δH 2.50 and δC 39.5 for DMSO-d6, coupling constants, J., are in hertz. All chemical shifts are given in ppm. Column chromatography was performed over a RP-18 reversed-phase silica gel (S-50 μm; YMC, Kyoto, Japan). Analytical HPLC (Agilent technologies, Santa Clara, CA, USA) was performed on an Agilent 1260 system equipped with a G1311C quaternary pump, a G1329B autosampler, a G1316A thermostated column compartment, and a G1314F variable wavelength detector coupled with an analytical workstation. Semipreparative HPLC was performed on an Agilent ProStar SD-1 pump connected with an Agilent ProStar 320 UV–Vis detector (at 360 nm), utilizing a Shim-Pack PREP-ODS column (250 mm × 21.2 mm, i.d., 10 μm, Shimadzu, Kyoto, Japan). HPLC-grade water was purified using a Milli-Q system (Millipore, Boston, MA, USA). All solvents used for the chromatographic separations were distilled before use.
10
Characterizing Polymer Molar Mass and Size
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The weight-average molar mass (Mw), the number-average molar mass (Mn), the intrinsic viscosity ([η]), the hydrodynamic radius (Rh), and the polydispersity index (PDI = Mw/Mn) were determined in the CMSP. A size-exclusion chromatography (SEC) system and a DAWN HELOS-II 8 multi-angle laser-light scattering (MALS) detector coupled with a ViscoStar-II viscometer and a refractive index (RI) Optilab T-rex detector (Wyatt Technology Corp., Santa Barbara, CA, USA) were used. The CMSP was dispersed in 100 mM NaNO3/0.02% NaN3 at 5 mg/mL and filtered (0.20 µm, Millipore Sigma, Saint Louis, MO, USA). An Agilent HPLC System (G1310B ISO pump, G1329B autosampler, and G1314F variable wavelength detector, Agilent Technologies, Inc., Santa Clara, CA, USA) and the columns Shodex OH-pak SBH-Q-804 and 805 (Shodex Showa Denco K.K., Tokyo, Japan) at a flow rate of 0.7 mL/min (100 mM NaNO3/0.02% NaN3) at 25 °C were employed. The ASTRA 6.1 software (Wyatt Technology Corp., Santa Barbara, CA, USA) was used.
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