Naïve CD4 T cells were purified from splenocytes of WT mice using Miltenyi naïve CD4 isolation kit. For Th1 induction in Figure 1A , naïve CD4 T cells were cultured on 24-well plates coated with 1 μg/ml of αCD3/CD28 (Biolegend) plus IL-12 (0.5 ng/ml) and AS1842856 (or DMSO) for 72h. For iTreg induction in Figure 5 , αCD3/CD28 (1 μg/ml) and recombinant mouse PD-L1-Ig chimera or human IgG1-Ig (10 μg/ml) (Biolegend) were used to coat plates. Naïve CD4 T cells were cultured on coated plates plus TGFβ (4 μg/ml) and AS1842856 (or DMSO) for 72h. For the expansion of human Th1 cells in Figure 4 C –D , PBMCs from treatment-naïve MS patients were plated in flasks for 2–4 hrs to remove adherent cells. The suspension cells were then collected and activated with plate-bound αCD3/CD28 plus IL-12 (0.5 ng/ml) and AS1842856 (0.1 μM) or DMSO for 3 days.
Recombinant mouse pd l1 ig chimera or human igg1 ig
Manufactured by BioLegend
Recombinant mouse PD-L1-Ig chimera or human IgG1-Ig is a laboratory reagent that consists of the extracellular domain of the mouse programmed death-ligand 1 (PD-L1) or the Fc region of human IgG1 fused to an Ig domain. This fusion protein can be used in various immunological research applications.
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Lab products found in correlation
2 protocols using recombinant mouse pd l1 ig chimera or human igg1 ig
1
Induction of Th1 and iTreg Cells from Murine Naive CD4+ T Cells
2
Induction and Expansion of Th1 and iTreg Cells
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Naïve CD4 T cells were purified from splenocytes of WT mice using Miltenyi naïve CD4 isolation kit. For Th1 induction in Figure 1A, naïve CD4 T cells were cultured on 24-well plates coated with 1 g/ml of αCD3/CD28 (Biolegend) plus IL-12 (0.5 ng/ml) and AS1842856 (or DMSO) for 72h.
For iTreg induction in Figure 5, αCD3/CD28 (1 g/ml) and recombinant mouse PD-L1-Ig chimera or human IgG1-Ig (10 μg/ml) (Biolegend) were used to coat plates. Naïve CD4 T cells were cultured on coated plates plus TGFβ (4 µg/ml) and AS1842856 (or DMSO) for 72h. For the expansion of human Th1 cells in Figure 4 C-D, PBMCs from treatment-naïve MS patients were plated in flasks for 2-4 hrs to remove adherent cells. The suspension cells were then collected and activated with plate-bound αCD3/CD28 plus IL-12 (0.5 ng/ml) and AS1842856 (0.1 µM) or DMSO for 3 days.
For iTreg induction in Figure 5, αCD3/CD28 (1 g/ml) and recombinant mouse PD-L1-Ig chimera or human IgG1-Ig (10 μg/ml) (Biolegend) were used to coat plates. Naïve CD4 T cells were cultured on coated plates plus TGFβ (4 µg/ml) and AS1842856 (or DMSO) for 72h. For the expansion of human Th1 cells in Figure 4 C-D, PBMCs from treatment-naïve MS patients were plated in flasks for 2-4 hrs to remove adherent cells. The suspension cells were then collected and activated with plate-bound αCD3/CD28 plus IL-12 (0.5 ng/ml) and AS1842856 (0.1 µM) or DMSO for 3 days.
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