Stock culture preparation of L. rhamnosus GG was carried out according to the procedure as previously described by Soukoulis et al. (2014a) . Six frozen culture beads were placed in MRS broth (Oxoid Ltd., Basingstoke, UK) and incubated at 37 °C (48 h) under anaerobic conditions in plastic jars containing AnaeroGen® (Oxoid Ltd., Basingstoke, UK). The final broth was transferred under aseptic conditions into 50 mL sterile centrifuge tubes (Sarstedt Ltd., Leicester, UK) and centrifuged at 3000 g for 5 min. Pellets were washed twice with phosphate buffer saline (PBS), Oxoid Ltd. Basingstoke, UK.
Sterile centrifuge tubes
Manufactured by Sarstedt
Sourced in United Kingdom
Sterile centrifuge tubes are laboratory equipment used for the collection, storage, and processing of biological samples. They are designed to withstand high-speed centrifugation while maintaining sample integrity. These tubes are made of high-quality materials and are sterilized to prevent contamination.
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Lab products found in correlation
2 protocols using sterile centrifuge tubes
1
Preparation of L. rhamnosus GG Stock Culture
2
Blood Staining of Drill Bits
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Forty-two of the drill bits were subjected to blood staining before bacterial contamination to test whether either surface would retain residual organic material, potentially enhancing bacterial adhesion. Leftovers from
blood samples of healthy horses collected in EDTA vacutainer tubes (Vacuette®, Greiner Bio-One GmbH) for pre-surgical routine laboratory testing were obtained from the Clinic for Horses. The fluted cutter part of each drill bit (50 mm) was immersed in 5 mL of equine blood for 1 minute in 15 mL sterile centrifuge tubes (Sarstedt) before fixation with 75% ethanol (Merck) for 10 seconds, and drill bits were allowed to dry at room temperature. This was repeated three times before these drill bits were returned to the remaining 48 drill bits for washing, sterilization and experiments with bacterial strains.
blood samples of healthy horses collected in EDTA vacutainer tubes (Vacuette®, Greiner Bio-One GmbH) for pre-surgical routine laboratory testing were obtained from the Clinic for Horses. The fluted cutter part of each drill bit (50 mm) was immersed in 5 mL of equine blood for 1 minute in 15 mL sterile centrifuge tubes (Sarstedt) before fixation with 75% ethanol (Merck) for 10 seconds, and drill bits were allowed to dry at room temperature. This was repeated three times before these drill bits were returned to the remaining 48 drill bits for washing, sterilization and experiments with bacterial strains.
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