Solvents used for this work include high performance liquid chromatography (HPLC) grades of hexane, methanol, acetone, ultrapure water and dichloromethane (Waters, Milford, MA, USA). Phthalate standards included di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethyl hexyl phthalate (DEHP), di-n-octyl Phthalate (DnOP), di-isononyl phthalate (DiNP), diisodecyl phthalate (DIDP) (AccuStandard Inc, (New Haven, CT, USA). Internal standard solutions, including phenanthrene-d10 and chrysene-d12 and standard surrogate solutions, 2-fluorobiphenyl and 4-terphenyl-d14, were obtained SUPELCO Inc. (Bellefonte, PA, USA).
Di 2 ethyl hexyl phthalate
Manufactured by AccuStandard
Sourced in United States
Di-2-ethyl hexyl phthalate is a chemical compound used as a plasticizer. It is a colorless, odorless liquid that is commonly used to increase the flexibility and durability of various materials, such as PVC plastics.
Automatically generated - may contain errors
Lab products found in correlation
Di n butyl phthalate, by AccuStandard (5 mentions)
Benzyl butyl phthalate, by AccuStandard (4 mentions)
Di n octyl phthalate, by AccuStandard (4 mentions)
Diisodecyl phthalate, by AccuStandard (3 mentions)
Di isononyl phthalate, by AccuStandard (3 mentions)
Dimethyl phthalate, by AccuStandard (2 mentions)
Diethyl phthalate, by AccuStandard (2 mentions)
4 terphenyl d 14, by Merck Group (1 mentions)
Phenanthrene d10, by Merck Group (1 mentions)
Chrysene d12, by Merck Group (1 mentions)
Dichloromethane, by Waters Corporation (1 mentions)
Milli q system, by Merck Group (1 mentions)
Hexane, by Thermo Fisher Scientific (1 mentions)
Methanol, by Thermo Fisher Scientific (1 mentions)
Ethyl acetate, by Thermo Fisher Scientific (1 mentions)
Acetone, by Thermo Fisher Scientific (1 mentions)
Methylene chloride, by Thermo Fisher Scientific (1 mentions)
6 protocols using di 2 ethyl hexyl phthalate
1
Phthalate Standards Quantification Protocol
2
Phthalate Compound Analysis Protocol
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PAE standards used in this present study include di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethyl hexyl phthalate (DEHP), di-n-octyl Phthalate (DnOP), di-isononyl phthalate (DiNP), and diisodecyl phthalate (DIDP) and were purchased from AccuStandard Inc. (New Haven, CT, USA). All solvents, including hexane, methanol, acetone, and dichloromethane, were high-performance liquid chromatography (HPLC) grades purchased from Waters Cooperation, USA. Additionally, solid-phase extraction cartridge Florisil (1 g 6cc, Chrom, and Sep) and an internal standard of 100 mg/L benzyl benzoate (BBZ) in n-hexane were purchased from Dr. Ehrenstorfer Gmbh (Augsburg, Germany). Anhydrous sodium sulfate (Tianjin Chengguang Chemical Reagent Co., Tianjin, China) was cleaned at 600 °C for 6 h and then kept in a desiccator before use.
3
Phthalate Extraction and Quantification
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Di-n-butyl phthalate (DnBP, 99.1%) and di-(2-ethylhexyl) phthalate (DEHP, 99.6%) were obtained from AccuStandard, Inc., New Haven, CT. Certified reference material (CRM) 119–100 (BNAs—Sandy Loam 6) and 136–100 (BNAs—Clay 1) were purchased from RT Corporation (Laramie, WY, one of the original Proficiency Test providers recognized by USEPA). Assay kits (Catalog numbers A045-3 for BCA/total protein content, A009 for vitamin C and A052 for inhibition and production of superoxide radical) were purchased from Jiancheng Bioengineering Institute, Nanjing, east China. Sodium chloride, monopotassium phosphate, disodium hydrogen phosphate, sodium hydroxide, potassium chloride, potassium hydroxide, elhylene diamine tetraacetic acid, acetic acid, absolute ethyl alcohol and acetone were analytical grade reagents purchased from the National Pharmaceutical Group Chemical Reagent Co., Ltd., Shanghai, China.
4
Phthalate Preparation and Analyses
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Reference PAEs including dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DnBP), butyl benzyl phthalate (BBP), di-n-octyl phthalate (DnOP), di (2-ethylhexyl) phthalate (DEHP) were purchased from AccuStandard Inc., USA. All solvents used for sample processing and analyses were chromatographic grade from Dikma Technologies Inc., USA.
5
Phthalate Esters Quantification Protocol
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All solvents, including hexane, methylene chloride, acetone, methanol, and ethyl acetate, which had purity levels of at least 99%, were of HPLC grade and obtained from Fisher Chemical Co. (Fair Lawn, OH, USA). Six PAE standard solutions used in this study including dimethyl phthalate (DMP), diethyl phthalate (DEP), di-n-butyl phthalate (DBP), butyl benzyl phthalate (BBP), di-2-Ethylhexyl phthalate (DEHP), and di-n-octyl phthalate (DNOP) were supplied by AccuStandard Inc. (New Haven, CT, USA, 99%). Solid-phase extraction membranes (ENVI-18 DSK) were obtained from Sigma-Aldrich Chemistry Co. (St. Louis, MO, USA). Anhydrous sodium sulfate (analytical grade, Beijing Chemical Reagent Co., Shanghai, China) was baked in a furnace oven (FP-40, China) at 550 °C for 5 h to remove any organics or water, then kept in a sealed desiccator prior to use. Water was prepared from a Milli-Q system (Millipore; Bedford, MA, USA). Glassware was cleaned with acetone first and then n-hexane.
6
Phthalate Quantification in Environmental Samples
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Solvents used in this work include hexane, methanol, acetone, and dichloromethane, which were high-performance liquid chromatography (HPLC) grade, purchased from Waters, U.S.A. Phthalate standards included, di-n-butyl phthalate (DBP), benzyl butyl phthalate (BBP), di-2-ethyl hexyl phthalate (DEHP), di-n-octyl Phthalate (DnOP), di-isononyl phthalate (DiNP), and diisodecyl phthalate (DIDP) were purchased from AccuStandard, U.S. Solid-phase extraction cartridge Florisil (1 g 6 cc, Chrom and Sep), an internal standard of 100 mg/L of benzyl benzoate (BBZ) in n-hexane were all purchased from Dr. Ehrenstorfer Gmbh (Augsburg, Germany).
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