Novocyte

Manufactured by BD

Sourced in United States

The NovoCyte is a flow cytometer designed for cell analysis. It provides accurate and reliable data on various cell properties, including size, granularity, and fluorescence intensity. The NovoCyte is capable of analyzing a wide range of sample types, making it a versatile tool for researchers and clinicians.

Automatically generated - may contain errors

Lab products found in correlation

Facscanto, by BD (1 mentions) Rnaase, by Thermo Fisher Scientific (1 mentions) Mitochondrial membrane potential detection kit, by BD (1 mentions) Annexin 5 fitc pi apoptosis detection kit 1, by BD (1 mentions) Golgiplug, by BD (1 mentions) Live dead fixable yellow stain, by Thermo Fisher Scientific (1 mentions) Il 13, by Thermo Fisher Scientific (1 mentions) Il 17a, by Thermo Fisher Scientific (1 mentions) Golgistop, by BD (1 mentions) Gata3, by Thermo Fisher Scientific (1 mentions) Foxp3 fjk 16s, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

ACEC NovoCyte flow cytometer (7 citations) NovoCyte 3000 flow cytometer (3 citations) NovoCyte flow cytometer (2 citations) NovoCyte 3000 (2 citations)

2 protocols using novocyte

Multiparametric Cell Analysis Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were collected and washed using PBS three times after compound or DMSO treatment on different days according to the requirements. For cell cycle detection, cells were fixed by 70% EtOH overnight at 4 ^oC. PI solution (200 μL) containing RNAase (Thermo) was added, the mixture was incubated for 15 min, then samples were filtered (40–50 μm nylon net) before detection. FITC Annexin V-PI Apoptosis Detection Kit I (BD Biosciences, 556547) was used for apoptosis detection. The procedures were as follows: 50 μL binding buffer, 2.5 μL PI, and 2.5 μL Annexin V were added to resuspended samples for 15 min. Then 150 μL binding buffer were added before centrifugation. Finally, the cells were resuspended in 200 μL binding buffer for FCM analysis. For CD11b (BD Biosciences, 555388), CD14 (BD Biosciences, 555397), CD41b (BD Biosciences, 555469), and CD61 (BD Biosciences, 555754) detection, antibodies were added to cells prior to incubation for 30 min, then they were washed by PBS three times before FCM detection. A mitochondrial membrane potential detection kit (BD Biosciences, USA) was selected for mitochondrial membrane potential detection and used according to the manufacturer’s instructions. FCM assays were conducted on FCM (ACEABIO | NovoCyte or BD FACSCanto, USA). Repeat three times for each sample.

Hou W., Wang Z.Y., Lin J, & Chen W.M. (2020). Induction of differentiation of the acute myeloid leukemia cell line (HL-60) by a securinine dimer. Cell Death Discovery, 6, 123.

+ Open protocol

+ Expand

Multiparameter flow cytometry profiling

Check if the same lab product or an alternative is used in the 5 most similar protocols

Single-cell suspension of lung and lymph node were stimulated with 100 ng/mL Phorbol-12-myristate 13-acetate (PMA), 750 ng/mL Ionomycin, 0.5 μL/mL GolgiStop (BD), 0.5 μL/mL GolgiPlug (BD) for 5 hours if mentioned. After excluding dead cells with LIVE/DEAD Fixable Yellow stain (Invitrogen), cells were pre-incubated with anti-FcγR III/II (Biolegend) for 15 minutes and labeled with the following antibody from Biolegend, unless otherwise specified: anti-B220 (RA3-6B2), CD3 (145-2C11), CD4 (GK1.5), CD8α (53-6.7), CD11b (M1/70), CD11c (N418), CD25 (PC61), CD44 (IM7), CD45 (30-F11), CD62L (MEL-14), CD69 (H1.2F3), CD103 (2E7), CD127 (SB/199), CCR7 (4B12), Dll1 (HMD1-3), Dll4 (HMD4-1), Gr-1 (RB6-8C5), I-A/I-E (M5/114.15.2), ST2 (DIH9). For Innate lymphoid cells staining, Lineage markers were anti-CD3, CD11b, B220, Gr-1, TER119. After 30 minutes of incubation at 4°C, cells were washed and proceed to intracellular staining.
For intracellular staining, cells were fixed and permeabilized with Transcription factors staining buffer set (eBioscience). Cells were labeled with antibody from eBioscience: Foxp3 (FJK-16s), IL-17A (eBio17B7), IL-13 (eBio13A), GATA3 (TWAJ), RORγt (AFKJS-9), GzmB (NGZB) for 30 minutes at room temperature. Flow cytometry data were acquired from LSR II (BD) or Novocyte (ACEA) flow cytometer and were analyzed with FlowJo software (TreeStar).

Ting H.A., Schaller M.A., de Almeida Nagata D.E., Rasky A.J., Maillard I.P, & Lukacs N.W. (2017). Notch ligand Delta-like 4 promotes regulatory T cell identity in pulmonary viral infection. Journal of immunology (Baltimore, Md. : 1950), 198(4), 1492-1502.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!