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Cd45 proteins

Manufactured by BioLegend
Sourced in United States

CD45 proteins are cell surface glycoproteins expressed on the majority of hematopoietic cells. They play a crucial role in the regulation of cellular signaling and function.

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2 protocols using cd45 proteins

1

Cell Culture and Protein Reagents

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Complete Medium (medium): RPMI-1640, DMEM medium, heat-inactivated fetal calf serum (FCS), penicillin, streptomycin, glutamine, sodium pyruvate, and HEPES obtained from Biological Industries (Kibbutz Beit Haemek, Israel). Mibefradil from Sigma-Aldrich (St. Louis, MO, USA). CD45 proteins were purchased from BioLegend (San Diego, CA, USA). Anti-human CD3 from eBioscience Inc. (ThermoFisher Scientific, Waltham, MA, USA).
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2

Cellular Metabolism Modulation Assay

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Complete Medium (medium): RPMI-1640, DMEM medium, heat-inactivated fetal calf serum (FCS), penicillin, streptomycin, glutamine, sodium pyruvate, and HEPES obtained from Biological Industries (Kibbutz Beit Haemek, Israel). Rotenone and 2-deoxy-d-glucose from Sigma-Aldrich (St. Louis, MO, United States). CD45 proteins were purchased from BioLegend. Anti-human CD3 from eBioscience Inc. (Thermo Fisher Scientific). Blebbistatin was purchased from Sigma-Aldrich (St. Louis, MO, United States).
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