T sod kit

The levels of corticosterone (CROT), total antioxidant capacity (T-AOC), malondialdehyde (MDA), and superoxide dismutase (T-SOD) in the serum were measured using kits. The CROT, T-AOC, and MDA kits were purchased from Shanghai Jianglai Biotechnology Co. Ltd. The T-SOD kit was purchased from Nanjing Jiancheng Bioengineering Institute.

Male SD rates (average body weight 293.61 g) were purchased from the Experimental Animal Center of the Academy of Military Medical Science. All animal studies were approved by the Institutional Care and Use Committee of the hospital.
The 10% chloral hydrate, 70 U/ml heparin sodium, recombinant human serum albumin (rHSA), total superoxide dismutase (T-SOD) Kit, peroxidase (POD) Kit, blood urea nitrogen (BUN) Kit and total protein Kit was purchased from Nanjing Jiancheng Bioengineering Institute. The Quantscript RT Kit and 2XTaq PCR Master Mix Kit were obtained from Tiangen Biotech (Beijing) Co., Ltd.

Red blood cell extract was prepared by centrifugation of portal vein blood with 2 ml, then the erythrocyte SOD activity was detected by the nitrite formation method according to the instruction of total superoxide dismutase (T-SOD) Kit (Nanjing Jiancheng Bioengineering Institute).
After all the experiments, 1g lung tissue of rats were collected and treated with cold 5% CaCl₂ solution, then put it in an ice water bath, the quality of the final concentration of 2%. Afterwards, centrifugation for 20 min in 4°C, 12 000 rpm, collecting supernatant, then using guaiacol colorimetric method to assay the activity of POD according to the instruction of peroxidase (POD) Kit (Nanjing Jiancheng Bioengineering Institute).