Imagemaster 2d platinum 7.0 analysis software

Diethyl nitrosamine, Proteo-Prep Immunoaffinity Albumin and IgG Depletion Kit, thiourea, dithiothreitol, iodine acetamide, sodium thiosulfate, and 2-dimensional gel electrophoresis standard protein were purchased from Sigma Company. Solid phase pH gradient dry strip (IPC strip pH 3-10 L, and 24 cm), interpublic buffer (pH : 3-10), ampholytes (pharmalyte, pH 3-10), low-molecular-weight protein, protein silver dye kits, urea, propylene phthalein amine, double propylene phthalein methylene amine, glycerin, glycine, nonidet P-40, 3-hydroxymethyl aminomethane, 3-[(3-bravery acyl amino propyl) dimethyl amine] propyl sulfonate, phenylmethylsulfonyl fluoride, Triton X-100, sodium dodecyl sulfate (SDS), EDTA, and filter column were purchased from Amershma Bioseiences Company. IPGhor isoelectric focusing (GE Healthcare), DALT-6 sodium dodecyl sulfate-polyacrylamide gel electropheresis (SDS-PAGE) electrophoresis apparatus, ImageScanner scanner, ImageMaster 2d Platinum 7.0 analysis software were purchased from GE Healthcare Company. ABI4800 tandem time-of-flight mass spectrometer (MALDI-TOF/TOF) is purchased from America ABI Co LTD. The primary and second antibodies of clusterin, NDRG2, and HSPa8 are purchased from Santa Cruz biotechnology Co, Ltd.