All the chemicals and reagents involved in the extraction processes, total polyphenolic content, antioxidant activity experiments and the chemical standards (1-hexanol, 2-Thujene, 3-octanone, 5-Amino-1-ethylprazole, Allylbenzene, Camphene, Caryophyllene oxide, Decanoic acid, Docosane, Germacrene D, Hexadecane, HexaDecanoic acid, Lavandulol, Limonene, Linalool, Linalool acetate, Nerol, Nerolidol, Ninanal, TetraDecanoic acid, Tricosane, α-Copaene, α-Humulene, α-Ter-pinene, β-Bourbonene, β-Caryophyllene, β-Copaene, β-Cubenene, β-Farnesene, β-Gurjunene, β-Myrcene, β-Pinene) used in the identification of phenolic and aromatic compounds from the extracts were acquired from Sigma Aldrich Company (St. Louis, MO, USA).
Lavandulol
Manufactured by Merck Group
Sourced in United States
Lavandulol is a chemical compound used in various laboratory applications. It is a clear, colorless liquid with a characteristic floral aroma. Lavandulol is commonly used as a reference standard or analytical tool in scientific research and analysis.
Automatically generated - may contain errors
Lab products found in correlation
Limonene, by Merck Group (2 mentions)
Linalool, by Merck Group (1 mentions)
β pinene, by Merck Group (1 mentions)
Caryophyllene oxide, by Merck Group (1 mentions)
Hexadecane, by Merck Group (1 mentions)
Decanoic acid, by Merck Group (1 mentions)
β caryophyllene, by Merck Group (1 mentions)
Nerol, by Merck Group (1 mentions)
Nerolidol, by Merck Group (1 mentions)
Tricosane, by Merck Group (1 mentions)
α humulene, by Merck Group (1 mentions)
Camphene, by Merck Group (1 mentions)
Docosane, by Merck Group (1 mentions)
β myrcene, by Merck Group (1 mentions)
Germacrene d, by Merck Group (1 mentions)
α copaene, by Merck Group (1 mentions)
β farnesene, by Merck Group (1 mentions)
Tetradecanoic acid, by Merck Group (1 mentions)
Hexadecanoic acid, by Merck Group (1 mentions)
α terpinene, by Merck Group (1 mentions)
3 protocols using lavandulol
1
Comprehensive Phytochemical Profiling of Extracts
2
Synthesis and Characterization of Sesquiterpene Acetates
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Lavandulyl acetate (LA), lavandulyl methylbutanoate (LMB), and neryl methylbutanoate (NMB) were synthesized (AD, Inc., Andong, Korea) (S1 Fig ). Briefly, LA and LMB were synthesized from the precursor, lavandulol (≥ 90%, Sigma-Aldrich Korea, Seoul, Korea) in dichloromethane, which was reacted with acetyl chloride to yield LA, and reacted with isovaleryl chloride (≥ 98%, Sigma-Aldrich Korea, Seoul, Korea) to yield LMB, respectively, under the catalytic activity of trimethylamine. NMB was used in a reaction with geraniol (> 98%, Sigma-Aldrich Korea) and isovaleryl chloride under the catalytic activity of trimethylamine. All products were extracted with ethyl acetate and produced 70 ~ 75% yields. The purity of the compounds was analyzed by gas chromatography (8860 GC, Agilent, Santa Clara, CA, USA) with a DB-1 column (15 m × 0.350 mm, Agilent), an oven temperature of 280°C, and a flow rate of 1.0 mL/min, and was determined to be 98.1% for LA, 98.1% for LMB, and 99.3% for NMB. All synthesized products were in the form of a racemic mixture. Two alarm pheromone components (decyl acetate and dodecyl acetate, 98%) were purchased from Sigma-Aldrich Korea and dissolved in hexane to prepare different concentrations.
3
Enzymatic Synthesis and Analysis of Terpenoids
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Enzymatic
assays were performed
in 1 mL of 50 mM HEPES, pH 7.8, containing 100 mM KCl, 7.5 mM MgCl2, 5% (w/v) glycerol, and 5 mM DTT. For each assay, 50 μg
of zFPS, ΔzFPS or ΔzFPS mutants was incubated at 32 °C for 2 h, with different combinations
of DMAPP, IPP, neryl diphosphate (NPP), and GPP (www.echelon-inc.com ) at 65
μM. The reaction products were then dephosphorylated by adding
20 units of alkaline phosphatase (www.neb.com ) and incubated at 37 °C for 1 h. The final products were extracted
three times, each by vortexing with 2 mL of pentane. After separating
the water phase by centrifugation, the obtained organic phase was
then concentrated to 10 μL. The products were analyzed on an
Agilent 5975C Series GC/MSD. The samples in pentane were injected
directly into the GC–MS system. Separation was assured with
a DB-5 capillary column (J&W Scientific; 30 m, 0.25 mm inside
diameter). The temperature program used was isothermal 70 °C
for 2 min, followed by a linear gradient (8 °C min–1) to 250 °C. Mass spectra from NIST Mass Spectral Library and
authentic standards were used for product confirmation. Authentic
standards including lavandulol, farnesol, limonene, and α-terpineol
were purchased from Sigma (www.sigmaaldrich.com ), and Z,Z-farnesol was purchased from Echelon (www.echelon-inc.com ).
assays were performed
in 1 mL of 50 mM HEPES, pH 7.8, containing 100 mM KCl, 7.5 mM MgCl2, 5% (w/v) glycerol, and 5 mM DTT. For each assay, 50 μg
of zFPS, ΔzFPS or ΔzFPS mutants was incubated at 32 °C for 2 h, with different combinations
of DMAPP, IPP, neryl diphosphate (NPP), and GPP (
μM. The reaction products were then dephosphorylated by adding
20 units of alkaline phosphatase (
three times, each by vortexing with 2 mL of pentane. After separating
the water phase by centrifugation, the obtained organic phase was
then concentrated to 10 μL. The products were analyzed on an
Agilent 5975C Series GC/MSD. The samples in pentane were injected
directly into the GC–MS system. Separation was assured with
a DB-5 capillary column (J&W Scientific; 30 m, 0.25 mm inside
diameter). The temperature program used was isothermal 70 °C
for 2 min, followed by a linear gradient (8 °C min–1) to 250 °C. Mass spectra from NIST Mass Spectral Library and
authentic standards were used for product confirmation. Authentic
standards including lavandulol, farnesol, limonene, and α-terpineol
were purchased from Sigma (
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