Serum interleukin 1 receptor antagonist (IL-1ra) concentrations were measured with Human IL-1ra/IL-1F3 Quantikine ELISA kits (R & D Systems, Minneapolis, USA). Serum TNF and IL-6 concentrations were measured with Proinflammatory Panel 1 Human Kit (Meso Scale Diagnostics, Maryland, USA), and C-reactive protein (CRP) was analyzed with Vascular Injury Panel 1 Human Kit (Meso Scale Diagnostics, Maryland, USA). Human Leptin, Insulin Kit (Meso Scale Diagnostics, Maryland, USA) was used to analyze serum leptin concentrations. All serum samples were tested in duplicate. The intra- and inter-assay coefficients of variation (CV) were 3.1% and 15.6% (IL-1ra), 4.2% and 7.0% (IL-6), 3.1% and 12.1% (TNF-a), 2.5% and 10.0% (CRP), and 6.6% and 8.9% (leptin), respectively.
Human il 1ra il 1f3 quantikine elisa kit
Manufactured by R&D Systems
Sourced in United States
The Human IL-1ra/IL-1F3 Quantikine ELISA kits are a quantitative sandwich enzyme immunoassay designed for the measurement of human Interleukin-1 receptor antagonist (IL-1ra) and Interleukin-1 Family Member 3 (IL-1F3) levels in cell culture supernates, serum, and plasma.
Automatically generated - may contain errors
Lab products found in correlation
Proinflammatory panel 1 human kit, by Mesoscale (2 mentions)
Vascular injury panel 1 human kit, by Mesoscale (2 mentions)
Prostaglandin e2 parameter assay kit, by R&D Systems (1 mentions)
Tgf β1 quantikine elisa kit, by R&D Systems (1 mentions)
Sensolyte plus 520 mmp 1 assay kit, by AnaSpec (1 mentions)
Infinite m200 reader, by Tecan (1 mentions)
Architect c8000 analyzer, by Abbott (1 mentions)
6 protocols using human il 1ra il 1f3 quantikine elisa kit
1
Inflammatory Biomarkers in Serum Analysis
2
Cytokine Profiling in THP-1 Cells
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Supernatants from THP-1 cells were collected at 0–48 h following LPS treatments. Secreted cytokine levels were assessed using human IL-1β/IL-1F2 Quantikine HS and human IL-1ra/IL-1F3 Quantikine ELISA kits (R&D Systems) according to the manufacturer’s instructions.
3
Quantification of Inflammatory Biomarkers
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Serum interleukin 1 receptor antagonist (IL-1ra) concentrations were measured with the Human IL-1ra/IL-1F3 Quantikine ELISA kits (R & D Systems, Minneapolis, USA). Serum TNF (TNF-α) and IL-6 concentrations were measured with the Proinflammatory Panel 1 Human Kit (Meso Scale Diagnostics, Maryland, USA), and C-reactive protein (CRP) was analyzed with Vascular Injury Panel 1 Human Kit (Meso Scale Diagnostics, Maryland, USA). The Human Leptin, Insulin Kit (Meso Scale Diagnostics, Maryland, USA) was used to analyze serum leptin concentrations. All serum samples were tested in duplicate. The intra- and inter-assay coefficients of variation (CV) were 3.1% and 15.6% (IL-1ra), 4.2% and 7.0% (IL-6), 3.1% and 12.1% (TNF-α), 2.5% and 10.0% (CRP), and 6.6% and 8.9% (leptin), respectively.
4
Quantification of Exosomal Factors
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The concentrations of TGF-β1, PGE2, and IL-1Ra in exosomes (Exo-FBS and Exo-RA) were assessed using human LAP (TGF-β1) Quantikine ELISA Kit, Prostaglandin E2 Parameter Assay Kit, and Human IL-1ra/IL-1F3 Quantikine ELISA Kit (R&D systems, Minneapolis, MN, USA), respectively.
5
Quantification of Pro-MMP1, PGE2, and IL1RN
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Pro-MMP1 was detected with the SensoLyte Plus 520 MMP-1 Assay Kit (AnaSpec, Freemont, CA, USA). PGE2 and IL1RN levels were measured with the Biotrend PGE2 Enzyme Immunoassay Kit (Biotrend Chemikalien, Köln, Germany) and the Human IL-1ra/IL-1F3 Quantikine ELISA Kit (R&D Systems, Wiesbaden, Germany) according to the manufacturer’s instructions. Fluorescence and absorbance values were measured on a Tecan Infinite M200 reader (Tecan, Crailsheim, Germany).
6
Biomarker Measurement in Frozen Serum
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Concentrations of hs-CRP were measured from frozen serum samples (−70 °C) using a latex immunoassay (Sentinel diagnostics, Milan, Italy) on Architect c8000 analyzer (Abbott Laboratories, Abbott Park, IL, USA), interleukin 6 and TNF-alpha concentrations with multiplex sandwich immunoassays (Milliplex High Sensitivity Human Cytokine kit, Millipore, Billerica, MA, USA) and IL-1Ra and high molecular weight adiponectin concentrations using enzyme linked immunosorbent assays (Human IL-1ra/IL-1F3 Quantikine ELISA Kit, R&D Systems, Inc., Minneapolis, MN, USA and Human HMW Adiponectin ELISA kit, Millipore, Billerica, MA, USA) for IL-1Ra and HMW adiponectin, respectively. Hs-CRP measurements of samples drawn at follow-up were conducted using the same method as mentioned above.
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