Icotinib standard substance (Beida Pharmaceutical co., Zhejiang, China) was configured as 10mmol/L solution in dimethyl sulfoxide (DMSO; Sigma, St. Louis, MO, USA). Erlotinib standard substance (Roche pharmaceutical Ltd., Basel, Switzerland) was diluted with DMSO as stocking solution of 100mmol/L. Pemetrexed (Qilu pharmaceutical company, Jinan, Shandong, China) was dissolved in physiological saline and configured as 10 mmol/L stocking solution. The solutions were stored at -20°C.
Pemetrexed
Manufactured by Qilu Pharmaceutical
Sourced in China
Pemetrexed is a lab equipment product manufactured by Qilu Pharmaceutical. It is a chemical compound used in various research and analytical applications.
Automatically generated - may contain errors
Lab products found in correlation
Cisplatin, by Qilu Pharmaceutical (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Paclitaxel, by Qilu Pharmaceutical (1 mentions)
Carboplatin, by Qilu Pharmaceutical (1 mentions)
Celltiter glo luminescent cell viability assay reagent, by Promega (1 mentions)
Pembrolizumab, by Merck Group (1 mentions)
Etoposide, by Qilu Pharmaceutical (1 mentions)
Ab8932, by Abcam (1 mentions)
Ab8805, by Abcam (1 mentions)
Ab2732, by Abcam (1 mentions)
Anti rabbit or anti mouse igg horseradish peroxidase hrp linked secondary antibodies, by Cell Signaling Technology (1 mentions)
Gapdh ab181602, by Abcam (1 mentions)
Ab54230, by Abcam (1 mentions)
Bcl2 associated x (bax), by Cell Signaling Technology (1 mentions)
Bcl 2, by Cell Signaling Technology (1 mentions)
H1975, by National Collection of Authenticated Cell Cultures (1 mentions)
Hcc827, by National Collection of Authenticated Cell Cultures (1 mentions)
5 protocols using pemetrexed
1
Preparation of Anticancer Drug Solutions
2
Camrelizumab and Chemotherapy for Cancer Treatment
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The eligible patients received the chemotherapy or Camrelizumab plus chemotherapy. The chemotherapy regimens included paclitaxel (Jiangsu Hengrui Pharmaceuticals Co., Ltd., Jiangsu, China), lobaplatin (Hainan Changan International Pharmaceutical Co., Ltd., Hainan, China), oxaliplatin (Jiangsu Hengrui Pharmaceuticals Co., Ltd., Jiangsu, China), cisplatin (Qilu Pharmaceutical Co., Ltd., Shandong, China), ifosfamide (Jiangsu Hengrui Pharmaceuticals Co., Ltd., Jiangsu, China) + carboplatin (Qilu Pharmaceutical Co., Ltd., Shandong, China), gemcitabine (Jiangsu Hengrui Pharmaceuticals Co., Ltd., Jiangsu, China) + paclitaxel, and pemetrexed (Qilu Pharmaceutical Co., Ltd., Shandong, China) + cisplatin. Camrelizumab (Jiangsu Hengrui Pharmaceuticals Co., Ltd., Jiangsu, China) was administered (200-mg) once a day. A cycle of treatment consisted of 21 days
3
Antibodies for Flow Cytometric Analysis of PD-1 Expression
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The following antibodies were used for flow cytometric analyses: anti-CD45-KO, anti-CD3-FITC, anti-CD4-PC5, anti-CD8-APC, anti-PD-1-PE, and PD-1 isotype. The anti-PD-1-PE and PD-1 isotype antibodies were purchased from BD Pharmingen (San Diego, CA, USA). All the other antibodies were purchased from Beckman Coulter (Brea, CA, USA). The purified mouse anti-human CD3 monoclonal Ab (anti-CD3 Ab) was purchased from BD Pharmingen. Pembrolizumab was purchased from Merck & Co. (Shanghai, China) and was used as an anti-PD-1 monoclonal Ab (anti-PD-1 Ab) in vitro. Human recombinant PD-L1 proteins were purchased from Sino Biotechnology (BDA, Beijing, China). The CellTiter-Glo Luminescent Cell Viability Assay reagent and the Cytotoxicity LDH Assay Kit were purchased from Promega (Madison, WI, USA) and Dojindo (Shanghai, China), respectively. Dicycloplatin (DCP),28 (link) albumin-bound paclitaxel, and docetaxel were purchased from Beijing Xingda Pharmaceutical Research Cooperation (Beijing, China), CSPC (Shanghai, China), and Sanofi (Hangzhou, China), respectively. Cisplatin, pemetrexed, and etoposide were all purchased from Qilu Pharmaceutical (Jinan, China).
4
Evaluation of Anti-Cancer Compounds
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Apatinib mesylate standard products were provided by Jiangsu Heng Rui Pharmaceutical Co. Ltd. (Jiangsu, China) and icotinib standard product was obtained from Zhejiang Beida Pharmaceutical Co., Ltd. (Zhejiang, China), pemetrexed was provided by Qilu Pharmaceutical Co., Ltd. (Shandong, China) and osimertinib standard was purchased from Selleck.cn. Three cell lines A549, HCC827 and H1975 were purchased from the cell bank of the Chinese Academy of Sciences (Shanghai, China) and cultured in RPMI media (HyClone) containing 10% fetal bovine serum (FBS, Gibco),100 U/mL penicillin (HyClone) and 100 ug/mL streptomycin (HyClone). Primary antibodies against AKT(ab8805), phosphor‐AKT(ab8932), ERK(ab54230), phospho‐ERK(ab201015), CD31(ab28364), mTOR(ab2732), phosphor‐mTOR(ab109268) and GAPDH(ab181602) were purchased from abcam (Cambridge, MA, USA). Primary antibodies against ki67 (9449) and anti‐rabbit or anti‐mouse IgG horseradish peroxidase (HRP)‐linked secondary antibodies were purchased from Cell Signaling Technology (Boston, MA, USA).
5
Metformin and Pemetrexed Synergistic Effects on NSCLC
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The human NSCLC cell lines A549, HCC827, and H1975 were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China) and were cultured as a monolayer in RPMI 1640 media (HyClone, Thermo Fisher Scientific Inc., UT; HCC827 and H1975) or DMEM media (HyClone; 5 mmol/L glucose, A549) supplemented with 10% fetal bovine serum (FBS, Gibco, Thermo Fisher Scientific Inc., NY), 100 U/mL penicillin (HyClone), and 100 μg/mL streptomycin (HyClone) in a 37°C humidified atmosphere containing 5% CO2. Metformin (1,1‐dimethylbiguanide monohydrochloride; Sigma‐Aldrich, St. Louis, MO) was dissolved in phosphate‐buffered saline (PBS) at a concentration of 1 mol/L and was stored at −20°C. Pemetrexed was provided by Qilu Pharmaceutical Co., LTD. (Shandong, China) and was dissolved in normal saline (NS) at a concentration of 10 mmol/L and was finally stored at −20°C. The two drugs were diluted by complete medium for each assay. The apoptotic markers Bcl‐2 and Bax were purchased from Cell Signaling Technology (Beverly, MA).
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