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Tetracaine

Manufactured by Alcon
Sourced in United States

Tetracaine is a local anesthetic used in various medical and laboratory applications. It is a topical agent that provides numbing and pain relief effects. Tetracaine is commonly used in eye care, dental procedures, and various laboratory testing and analysis.

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3 protocols using tetracaine

1

Rat-based Ophthalmic Anesthesia Protocol

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The use of rats followed the animal study guidelines of the Association of Research in Vision and Ophthalmology (ARVO) and was approved by the Administrative Panel on Laboratory Animal Care (APLAC) at Stanford University. Both male and female Long Evans rats (6 weeks of age; body weight 150 - 200 g; Charles River, Wilmington, MA) were housed at constant temperature, with a 12 h light/dark cycle, and food and water available ad libitum. Before all treatments, rats were anesthetized with intraperitoneal injection of a mixture of ketamine hydrochloride (75 mg/kg; Hospira, Inc., Lake Forest, IL) and xylazine (5 mg/kg; Bedford Laboratories, Bedford, OH). The corneas were topically anesthetized with tetracaine 0.5% (Alcon Laboratories, Inc. Fort Worth, TX), and the pupils dilated with tropicamide 1% (Akorn, Inc., Lake Forest, IL) and phenylephrine hydrochloride 2.5% (Akorn, Inc.).
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2

Femtosecond-Assisted Intrastromal Corneal Ring Segment Implantation

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All surgical procedures were performed by the same experienced surgeon (PL) under topical anaesthesia (combination of tetracaine 0.1% and oxybuprocaine 0.4%, Alcon, Fort Worth, TX, USA). The femtosecond laser platform FS 200 (Alcon Laboratories, Inc, Fort Worth, TX, USA) was used to create the corneal incision and tunnels. The tunnels were created at 70% of corneal depth ensuring that at least 100 μm of corneal tissue was present under the tunnel. Keraring segments (Mediphacos, Belo Horizonte, Brazil) were implanted in all cases, with a selection of the number of segments to implant as well as their diameter (SI5 or SI6), arc length and thickness according to the manufacturer’s nomogram and the scotopic pupil size measured. Table 1 summarizes the parameters used in the femtosecond laser platform for the different type of ring segments implanted. Topical antibiotics (Moxifloxacin 0.3%, Alcon Laboratories Inc., Fort Worth, TX, USA) were instilled at the time of implanting ICRS and at the end of the surgery. No stitches and/or therapeutic contact lenses were needed. A plastic protector was placed in the implanted eye to avoid the manipulation of the operated eye. Topical tobramycin 0.3% combined with sodium dexamethasone phosphate 0.1% (Tobradex, Alcon Laboratories Inc, Fort Worth, TX, USA) was instilled in the operated eye 4 times per day for 15 days.
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3

Laser-induced Choroidal Neovascularization in Mice

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Mice were anesthetized via intraperitoneal (IP) delivery of ketamine (90 mg/kg; Akorn, Lake Forest, IL, USA)/xylazine (12 mg/kg; Akorn) cocktail. Eyes were anesthetized with 1 drop of 0.5% tetracaine (Alcon, Fort Worth, TX, USA). Pupils were dilated with 1 drop of 2.5% phenylephrine (Akorn) and 0.5% tropicamide (Akorn). The whiskers were trimmed with scissors. A subcutaneous injection of meloxicam (1 mg/kg; Henry Schein Animal Health, Melville, NY, USA) was given for pain control. Mice were moved to the slit lamp stage and a cover slip was coupled to the cornea using Goniosoft (OCuSOFT, Rosenberg, TX, USA) to allow direct retinal visualization. Mouse eyes were treated with 4 (CNV area quantitation) or 8 (flow cytometry) focal laser burns (75 um, 110 mW, 100 msec) in each eye using an IRIDEX (Mountain View, CA, USA) 532 nm argon ophthalmic laser delivered via a Zeiss (Oberkochen, Germany) slit lamp. IP injections of propranolol (20 mg/kg) or PBS were performed daily during the course of experiments.
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