Metal concentrations were determined by inductively coupled plasma atomic emission spectroscopy (ICP-AES) (Shimadzu ICP 9000 with mini plasma torch) in axial reading mode. SufU was mineralized by incubation in 65% (v/v) HNO3 for 16 h at 60 °C. The volume was brought up to 6 mL with pure water. Standard solutions of Ni, Co, Mo, Mg, and Fe for atomic absorption spectroscopy (Sigma-Aldrich, Saint-Quentin-Fallavier, France) were used for quantification (calibration curve between 1.9 and 5000 μg L−1 in 10% HNO3 (Fluka, Illkirch-Graffenstaden, France).
Icp 9000
Manufactured by Shimadzu
Sourced in Japan
The ICP 9000 is an inductively coupled plasma (ICP) spectrometer manufactured by Shimadzu. It is designed for the analysis of trace elements in a wide range of sample types. The ICP 9000 utilizes a high-frequency plasma to atomize and excite the sample, allowing for the detection and quantification of multiple elements simultaneously.
Automatically generated - may contain errors
Lab products found in correlation
Mini plasma torch, by Shimadzu (4 mentions)
Standard solution for aas, by Merck Group (2 mentions)
Ytterbium solution standard for aas, by Merck Group (2 mentions)
Waters 2695, by Waters Corporation (1 mentions)
Inova 500 mhz spectrometer, by Agilent Technologies (1 mentions)
Tecnai g2 f20, by Malvern Panalytical (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
9 protocols using icp 9000
1
Quantifying Metal Concentrations by ICP-AES
2
Soil Chemical Properties Determination
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The soil pH value was determined with a glass electrode using a soil-to-water ratio of 1:2.5 (w/v). OM was determined by a K2Cr2O7 oxidation-reduction titration method and the Kjeldahl method was used for TN estimation (Guimaraes et al., 2013 (link)). TP and TK were digested by HF-HClO4 and determined by molybdenum-blue colorimetry and flame photometry, respectively (Shen et al., 2008 (link)). AHN in soil was determined based on the transformation of hydrolyzed nitrogen into ammonia nitrogen with sodium hydroxide (Li et al., 2013 ). AP in soil was extracted with sodium bicarbonate and determined using the molybdenum blue method (Hedley et al., 1982 (link)). AK in soil was extracted with ammonium acetate and determined with inductively coupled plasma spectrophotometry (ICP-9000, Shimadzu, Japan) (Fukuda et al., 2017 (link)).
3
Biochemical Analyses of Rice Roots
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Root activity was measured using the α-naphthylamine oxidation method [68 ], and relative conductivity was measured using a DDS-307 conductivity meter (LeiCi, Shanghai, China). The MDA content was determined using the thiobarbituric acid method [69 (link)]. The O2•− production rate was determined using the hydroxylamine oxidation method [70 (link)]. The H2O2 content was determined using a kit (Grace, Jiangsu, China). To measure the content of Na+ and K+, the roots were washed three times with deionized water and dried at 80 °C for 3 days. All samples were weighed and digested with 5 mL of concentrated HNO3. Subsequently, the sample was diluted with deionized water to 25 mL, kept for 1–2 days, and then analyzed by Inductively coupled plasma atomic emission spectroscopy (ICP-AES; ICP9000, Shimadzu, Japan) [71 (link)]. The ABA content in rice roots and leaves was measured using high-performance liquid chromatography (HPLC; Waters 2695, USA) [72 (link)].
4
Zinc Content Quantification in Cellular Extracts
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Shimadzu ICP 9000 (with Mini plasma Torch in axial reading mode) was used to measure the zinc content. Standard solution for AAS (Sigma Aldrich) was used to generate the calibration curve between 10 to 500 µg/L in pure water with 1% of HNO3 (Fluka). Samples were routinely incubated in HNO3 10% ON at RT. Briefly, 500 µl of each cytosolic crude extract was incubated with 90 µl of HNO3 65% ON at RT. The sample was centrifuged at 13,000 rpm for 5 min. Before to be measured pure water was added to the supernatant extemporaneously to obtain a final volume of 7 ml. Ytterbium solution standard for AAS (Sigma Aldrich) was used as an internal standard to prevent calibration drift and fluidic perturbation. The result was express in µg/L [Zn] per mg of protein.
5
Characterization of HPEI-IBAm Nanoparticles
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1H NMR spectra of the HPEI-IBAm were obtained on a Varian INOVA 500MHz spectrometer. Ultraviolet-visible (UV-vis) absorption spectra were performed using a T6 UV/Vis Spectrophotometer (Purkinje General, Beijing, China). Transmission electron microscopy (TEM) analysis was performed on a Philips (Amsterdam, Netherlands) TECNAI G2 F20 operating at 200 kV. The size of the nanoparticles was measured by dynamic light scattering on a Malvern Instruments (Malvern, UK) Zetasizer Nano-ZS90 at 25 °C. The concentration of Ag+ was determined by an Inductively Coupled Plasma spectrometer (ICP-9000, Shimadzu, Kyoto, Japan).
6
Mineral Content Analysis of Pigments
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Pigments at just below the Lethal Dose 20 (LD 20) concentration for J774A1 macrophages, i.e. 2.5 µg/ml and 500 µg/ml, were mineralized in aqua regia (Mixture of nitric acid and hydrochloric acid, optimally in a molar ratio of 1:3). Samples were diluted in 10% nitric acid prior to analysis by inductively coupled plasma atomic emission spectroscopy (ICP-AES) on a Shimadzu ICP 9000 with Mini plasma Torch instrument used in axial reading mode. A standard range of cobalt, zinc and aluminum (from 3.9 µg/L to 10 mg/L) was prepared for quantification.
7
Metal Analysis in R. rubrum Cultures
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15 ml of R. rubrum culture exposed or not to CO were centrifuged at 5000 rpm for 10 minutes. 5.4 ml of the supernatant were mineralized in the presence of 0,6 ml nitric acid (65%) at 60°C for 1hour. The cell pellet was washed in PBS supplemented with 10 mM EDTA before being lysed and mineralized with 1.2ml of 65% nitric acid for 3h at 60°C; the volume was completed to 6 mL with pure water. Metal concentration was analyzed by inductively coupled plasma atomic emission spectroscopy (ICP-AES) (Shimadzu ICP 9000 with mini plasma torch in axial reading mode). Standard solutions of Ni, Co, Mo, Mg, and Fe for atomic absorption spectroscopy (Sigma Aldrich) were used for quantification (calibration curve between 1.9 to 5000 μg L -1 in 10% HNO3 (Fluka)). The analyses were performed on three cultures from each condition (with or without CO) and each result was normalized to the number of bacteria as estimated from the OD at 650nm.
8
Phosphorus Content Quantification in SiNWs
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Inductively coupled plasma atomic emission spectroscopy (ICP-AES) (Shimadzu ICP 9000 with a Mini plasma Torch in axial reading mode) was used to measure the phosphorus content. Standard solutions of P for atomic absorption spectroscopy (Sigma Aldrich) were used for quantification (calibration curve between 7.8 and 2000 µg L -1 with 10% HNO 3 (Fluka)). SiNWs (10 mg) were dissolved in NaOH aqueous solution (4 M, 0.5 mL) in a plastic vial. After 1 hour incubation in an ultrasound bath, the solution was centrifuged (2000g, 2 minutes) to remove the gold nanoparticles and diluted in 6 mL aqueous HNO 3 10%. ICP-AES was performed on this solution and a 1/100 dilution in HNO 3 10%. Results were obtained in (mg of P)/(mg of SiNW).
9
Quantifying Zinc in Cytosolic Extracts
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Shimadzu ICP 9000 (with Mini plasma Torch in axial reading mode) was used to measure the zinc content. Standard solution for AAS (Sigma Aldrich) was used to generate the calibration curve between 10 to 500 µg/L in pure water with 1% of HNO3 (Fluka). Samples were routinely incubated in HNO3 10% ON at RT. Briefly, 500µl of each cytosolic crude extract was incubated with 90µl of HNO3 65% ON at RT. The sample was centrifuged at 13,000 rpm for 5 min. Before to be measured pure water was added to the supernatant extemporaneously to obtain a final volume of 7 ml. Ytterbium solution standard for AAS (Sigma Aldrich) was used as an internal standard to prevent calibration drift and fluidic perturbation. The result was express in µg/L [Zn] per mg of protein.
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