Cultured BMM were lysed in CHAPS buffer (Thermo Fisher Scientific) containing protein inhibitors. Ten to 40 μg of protein per sample was loaded to 10% SDS–polyacrylamide gel electrophoresis gels followed by transfer to polyvinylidene difluoride membranes. The membranes were then blocked with 5% nonfat milk in 1× tris-buffered saline plus 0.05% Tween 20 for 2 hours at room temperature and incubated with corresponding primary antibodies. Primary antibodies are as follows: anti-KDM5C (A301-034A, Bethyl Laboratories) or anti–β-actin (4967, Cell Signaling Technology) and OXPHOS rodent Western blots antibody cocktail (45-8099, Thermo Fisher Scientific). Blots were developed using the SuperSignalt West Dura Extended Duration Substrate (Thermo Fisher Scientific) and imaged using the ChemiDoc MP Imaging System (Bio-Rad).
Anti kdm5c
Manufactured by Fortis Life Sciences
Anti-KDM5C is a reagent that targets the KDM5C protein. KDM5C is a histone demethylase enzyme that plays a role in gene expression regulation. Anti-KDM5C can be used to detect and study the KDM5C protein in various experimental applications.
Automatically generated - may contain errors
Lab products found in correlation
Chaps buffer, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Cell Signaling Technology (1 mentions)
Chemidoc mp imaging system, by Bio-Rad (1 mentions)
Anti h3k4me3, by Cell Signaling Technology (1 mentions)
Anti ubiquitin, by Proteintech (1 mentions)
Anti flag, by Proteintech (1 mentions)
Anti hemagglutinin ha, by Proteintech (1 mentions)
Anti rabbit antibodies, by Cell Signaling Technology (1 mentions)
Anti zmynd8, by Proteintech (1 mentions)
2 protocols using anti kdm5c
1
Western Blot Analysis of KDM5C
2
Immunoblotting Assay for Protein Interactions
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The following primary antibodies were used: anti-ZMYND8 (Proteintech), anti-ACTB (Actin Beta) (Proteintech), anti-hemagglutinin (HA; Proteintech), anti-Flag (Proteintech), anti-ubiquitin (Proteintech), anti-H3K4me3 (Cell Signaling Technology), anti-KDM5C (Bethyl), anti-ZNF592 (Bethyl), and anti-ZNF687 (Bethyl). The HRP-linked anti-mouse (1/5000) and anti-rabbit antibodies (1/5000) were purchased from Cell Signaling Technology.
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